##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805257_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18533010
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.35879379550327	37.0	37.0	37.0	35.0	37.0
2	36.36433347847975	37.0	37.0	37.0	35.0	37.0
3	37.89704948089922	39.0	39.0	39.0	35.0	39.0
4	37.98766298620677	39.0	39.0	39.0	35.0	39.0
5	37.9141264155148	39.0	39.0	39.0	35.0	39.0
6	37.948792074250214	39.0	39.0	39.0	35.0	39.0
7	37.839787654568795	39.0	38.0	39.0	35.0	39.0
8	38.81920195370315	40.0	39.0	40.0	36.0	40.0
9	38.788963044858875	40.0	39.0	40.0	36.0	40.0
10	38.68320337603012	40.0	39.0	40.0	36.0	40.0
11	38.74951634947588	40.0	39.0	40.0	36.0	40.0
12	38.67085287279293	40.0	39.0	40.0	36.0	40.0
13	38.6308171743284	40.0	39.0	40.0	36.0	40.0
14	38.73274093091192	40.0	39.0	40.0	36.0	40.0
15	38.622166987445645	40.0	39.0	40.0	36.0	40.0
16	38.6794032377903	40.0	39.0	40.0	36.0	40.0
17	38.63566425529366	40.0	39.0	40.0	36.0	40.0
18	38.4992366593446	40.0	38.0	40.0	36.0	40.0
19	38.47727649205391	40.0	38.0	40.0	36.0	40.0
20	38.3516770346533	40.0	38.0	40.0	35.0	40.0
21	38.02548754897342	40.0	38.0	40.0	35.0	40.0
22	38.093111858246445	40.0	38.0	40.0	34.0	40.0
23	38.09336157483323	40.0	38.0	40.0	35.0	40.0
24	37.97454892648307	40.0	38.0	40.0	34.0	40.0
25	37.8503955374761	40.0	38.0	40.0	34.0	40.0
26	37.853713778819525	40.0	38.0	40.0	34.0	40.0
27	37.43645943103684	39.0	37.0	40.0	33.0	40.0
28	37.54186966930898	39.0	37.0	40.0	34.0	40.0
29	37.46433191370425	39.0	37.0	40.0	33.0	40.0
30	37.37731485603256	39.0	37.0	40.0	33.0	40.0
31	37.240580779916485	39.0	37.0	40.0	33.0	40.0
32	36.898501970268185	38.0	36.0	40.0	33.0	40.0
33	36.906405003828304	38.0	36.0	40.0	33.0	40.0
34	36.82691521776549	38.0	36.0	40.0	33.0	40.0
35	36.56327412546586	38.0	36.0	40.0	32.0	40.0
36	36.43742014923642	38.0	36.0	40.0	31.0	40.0
37	36.113598870340006	38.0	35.0	39.0	31.0	40.0
38	36.097378677289875	38.0	35.0	39.0	31.0	40.0
39	35.926644997223875	38.0	35.0	39.0	31.0	40.0
40	35.75820570970393	38.0	35.0	39.0	31.0	40.0
41	35.60296303730479	37.0	34.0	39.0	30.0	40.0
42	35.34692125024483	37.0	34.0	38.0	30.0	40.0
43	35.133162556972664	37.0	34.0	38.0	30.0	40.0
44	35.12012857058837	37.0	34.0	38.0	30.0	40.0
45	34.877131885214546	36.0	34.0	38.0	29.0	40.0
46	33.90688555178031	36.0	33.0	38.0	26.0	39.0
47	33.80255597984353	36.0	32.0	38.0	26.0	39.0
48	33.982602933900104	36.0	32.0	38.0	27.0	39.0
49	33.9633407093613	35.0	32.0	38.0	28.0	39.0
50	33.818976895819944	35.0	32.0	38.0	28.0	39.0
51	33.32552132654113	35.0	32.0	38.0	26.0	39.0
52	33.068626790791136	34.0	31.0	37.0	26.0	38.0
53	35.35761697641128	37.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	9.0
12	49.0
13	177.0
14	517.0
15	1313.0
16	2390.0
17	3510.0
18	5107.0
19	7598.0
20	12146.0
21	18751.0
22	27047.0
23	38007.0
24	51729.0
25	67528.0
26	86738.0
27	109116.0
28	137090.0
29	171067.0
30	213872.0
31	268515.0
32	345163.0
33	459537.0
34	653702.0
35	1020265.0
36	1890755.0
37	4459263.0
38	7919694.0
39	562354.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.88593434094084	32.57031642458511	24.206904329086317	25.33684490538774
2	27.51513650507931	26.995533914890242	25.514301238708658	19.97502834132178
3	23.208237625728362	24.82562195779315	26.39316549227567	25.572974924202814
4	20.979630400026764	28.77936179821842	25.17378990244974	25.067217899305078
5	24.928416916626066	28.959310980785098	24.705997568662617	21.40627453392622
6	25.691239577381115	25.6507118919161	26.344263559993763	22.313784970709023
7	23.917539568586	26.437502596717966	27.786921822197257	21.85803601249878
8	24.160468267162216	25.677501927641543	26.861367905159494	23.300661900036744
9	24.054559944660905	25.316869736756196	27.712708297249073	22.915862021333826
10	22.61722191915938	26.430018653203124	28.265133402507203	22.687626025130296
11	22.40857259560104	28.325280135282938	26.546211327787557	22.719935941328472
12	22.535340994258355	26.080134851273485	28.140415399333406	23.244108755134757
13	22.796183674427414	26.503352666404435	25.820025996856423	24.880437662311735
14	22.93548646442213	28.408806772348367	25.817819123822844	22.83788763940666
15	22.82125245710222	27.980738153165625	25.774021597139374	23.423987792592786
16	23.255758238947692	26.139822942954222	25.638857368554813	24.965561449543276
17	25.37861901547563	25.809283003678303	25.674793247292264	23.137304733553805
18	23.045916448542357	26.04777097729942	25.68689597642261	25.219416597735606
19	23.464504686502625	28.008003017318828	25.603736252233176	22.92375604394537
20	25.285504081635956	26.051073193183406	25.77216544964903	22.891257275531608
21	23.55414473957549	27.847511008735225	25.626452475879525	22.97189177580976
22	25.072608281115695	25.844479660886172	26.062139933016816	23.020772124981317
23	23.14555487748617	26.03403872333744	28.007889705989474	22.812516693186915
24	23.063220707267735	27.68395959425911	25.870449538418207	23.38237016005495
25	23.17933784096593	26.016006034637655	25.806153452677144	24.998502671719272
26	23.298973021651637	27.017721352332945	26.862215042240845	22.821090583774573
27	23.813983805113146	25.472586482174243	27.837938899293746	22.875490813418867
28	24.86321973602777	25.73084998065614	26.420565250868584	22.985365032447508
29	24.95035614829971	25.65997644203505	26.49076431729115	22.89890309237409
30	24.76880441978934	25.56333806543028	26.464864584867758	23.20299292991263
31	24.11509517342299	26.049972454555416	26.519545394946636	23.315386977074958
32	24.15702576106094	26.22359239001112	26.52526491919014	23.094116929737805
33	23.71013666965053	25.988671025375805	26.694168945033752	23.607023359939912
34	23.624392367996347	26.157521093443535	26.694336214138985	23.523750324421126
35	23.846871069513263	26.092237580403832	26.72266944225466	23.33822190782825
36	23.638750532158564	25.97838667329268	26.721023730090256	23.661839064458498
37	23.748058194540445	25.978640274839325	26.57618487229004	23.697116658330188
38	23.81026611435487	26.088832844745674	26.751790453898206	23.34911058700125
39	23.791747805672152	25.927466720192783	26.750430717945978	23.53035475618909
40	23.683794483464908	26.148029920665884	26.65923128515012	23.50894431071909
41	23.73315505684182	26.103018344025063	26.75792005723841	23.405906541894705
42	23.692282041611158	25.991536183275137	26.75598297308424	23.56019880202946
43	23.71925013799701	26.100552473667253	26.641716591098803	23.53848079723693
44	23.769975843103737	26.001372685818442	26.71200738574036	23.51664408533746
45	23.702604164137394	25.81937850354584	26.741101418495973	23.7369159138208
46	23.8344553852828	26.042310450380157	26.522394365513215	23.600839798823827
47	23.940601121997993	25.990834732188674	26.579357589511904	23.489206556301433
48	23.70733626108225	25.8779118988227	26.68135397326176	23.73339786683329
49	23.712953265551576	26.152017400303567	26.55178516603617	23.58324416810869
50	23.81385430645103	26.04029242956217	26.661265493300874	23.484587770685927
51	23.952957452674983	25.885320301451305	26.56839336945267	23.593328876421047
52	23.779850116090152	26.097994875090446	26.48643150788782	23.63572350093158
53	23.859659062397313	26.08823930921097	26.466985125459924	23.585116502931797
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2173.0
1	1562.5
2	952.0
3	801.0
4	650.0
5	661.0
6	672.0
7	777.0
8	1159.0
9	1436.0
10	2075.5
11	2715.0
12	4417.0
13	6119.0
14	8077.5
15	10036.0
16	14978.0
17	19920.0
18	28514.5
19	37109.0
20	51390.0
21	65671.0
22	128859.5
23	192048.0
24	182763.0
25	195857.5
26	218237.0
27	249956.0
28	281675.0
29	319237.0
30	356799.0
31	391406.5
32	426014.0
33	474894.0
34	523774.0
35	586903.5
36	650033.0
37	714576.0
38	779119.0
39	839328.0
40	899537.0
41	968257.5
42	1100337.5
43	1163697.0
44	1217297.5
45	1270898.0
46	1310144.0
47	1349390.0
48	1363979.0
49	1378568.0
50	1372317.0
51	1366066.0
52	1333634.5
53	1301203.0
54	1249991.5
55	1198780.0
56	1115928.5
57	1033077.0
58	937727.0
59	745577.5
60	648778.0
61	567896.0
62	487014.0
63	415519.5
64	344025.0
65	280299.5
66	216574.0
67	171229.0
68	125884.0
69	95438.5
70	64993.0
71	47192.5
72	29392.0
73	21764.0
74	14136.0
75	10493.0
76	4854.0
77	2858.0
78	2044.5
79	1231.0
80	982.0
81	733.0
82	520.5
83	308.0
84	193.0
85	78.0
86	53.0
87	28.0
88	26.0
89	24.0
90	17.0
91	10.0
92	7.5
93	5.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.853301E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.20812148670924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.13446742431246	35.25675290457034
2	13.941522810153351	13.441892506832016
3	4.7681804395895835	6.89595065706856
4	2.337915351804436	4.5082602922172335
5	1.3225967871373427	3.187995329612417
6	0.8251646862167006	2.3867783663806206
7	0.5582541895457959	1.8838670053061692
8	0.4153902860200663	1.6020148298283403
9	0.3187513728392722	1.3829764415301886
>10	2.174762348292141	20.002659929535717
>50	0.16055328038337785	5.224754559921578
>100	0.04120595331836715	3.3146148244833427
>500	0.001040076356410514	0.3148984180322842
>1k	1.6059781640998736E-4	0.15086046421263083
>5k	2.2930809456059664E-5	0.08203553711751631
>10k+	1.1465404728029829E-5	0.3636879333509941
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65799	0.35503676952637486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3489443970515313E-4	0.0	0.0	1.079155517641225E-5	5.395777588206125E-6
2	1.510817724697715E-4	0.0	0.0	1.079155517641225E-5	5.395777588206125E-6
3	1.672691052343899E-4	0.0	0.0	1.079155517641225E-5	5.395777588206125E-6
4	1.7806066041080212E-4	0.0	0.0	1.079155517641225E-5	5.395777588206125E-6
5	2.1583110352824502E-4	0.0	0.0	1.079155517641225E-5	1.6187332764618377E-5
6	2.2662265870465725E-4	0.0	0.0	1.6187332764618377E-5	2.15831103528245E-5
7	2.5360154664568786E-4	0.0	0.0	1.6187332764618377E-5	2.15831103528245E-5
8	2.5899732423389403E-4	0.0	0.0	1.6187332764618377E-5	3.777044311744288E-5
9	2.6978887941030627E-4	0.0	0.0	1.6187332764618377E-5	5.935355347026738E-5
10	3.02163544939543E-4	0.0	0.0	3.2374665529236754E-5	6.474933105847351E-5
11	3.0755932252774914E-4	0.0	0.0	3.2374665529236754E-5	7.554088623488576E-5
12	3.237466552923675E-4	0.0	0.0	3.777044311744288E-5	8.093666382309187E-5
13	3.2914243288057366E-4	0.0	0.0	3.777044311744288E-5	8.093666382309187E-5
14	3.5612132082160424E-4	0.0	0.0	4.3166220705649E-5	8.093666382309187E-5
15	4.046833191154594E-4	0.0	0.0	4.8561998293855125E-5	8.093666382309187E-5
16	4.2087065188007775E-4	0.0	0.0	5.3957775882061255E-5	1.0251977417591638E-4
17	4.478495398211084E-4	0.0	0.0	5.935355347026738E-5	1.1331132935232863E-4
18	4.8022420535034514E-4	0.0	0.0	5.935355347026738E-5	1.1331132935232863E-4
19	5.395777588206125E-4	0.0	0.0	5.935355347026738E-5	1.2949866211694702E-4
20	6.151186450554983E-4	0.0	0.0	5.935355347026738E-5	1.4029021729335925E-4
21	6.744721985257657E-4	0.0	0.0	6.474933105847351E-5	1.4029021729335925E-4
22	7.500130847606514E-4	0.0	0.0	6.474933105847351E-5	1.6187332764618374E-4
23	8.525328589365677E-4	0.0	0.0	6.474933105847351E-5	1.672691052343899E-4
24	9.658441882888964E-4	0.0	0.0	6.474933105847351E-5	1.672691052343899E-4
25	0.0010467808521119884	0.0	0.0	6.474933105847351E-5	1.672691052343899E-4
26	0.0011439048486996985	0.0	0.0	6.474933105847351E-5	1.72664882822596E-4
27	0.0013219655091105008	0.0	0.0	6.474933105847351E-5	1.8345643799900826E-4
28	0.0014784430591684784	0.0	0.0	7.554088623488576E-5	2.0503954835183276E-4
29	0.0016780868299321049	0.0	0.0	7.554088623488576E-5	2.1043532594003888E-4
30	0.0018183770472254641	0.0	0.0	8.093666382309187E-5	2.2662265870465725E-4
31	0.0020072292628126784	0.0	0.0	8.093666382309187E-5	2.374142138810695E-4
32	0.0022338519215173358	0.0	0.0	8.6332441411298E-5	2.374142138810695E-4
33	0.00266011835098562	0.0	0.0	8.6332441411298E-5	2.4820576905748175E-4
34	0.00285436634416104	0.0	0.0	8.6332441411298E-5	2.4820576905748175E-4
35	0.0030971763356303157	0.0	0.0	1.0251977417591638E-4	2.6439310182210015E-4
36	0.003313007439158561	0.0	0.0	1.1331132935232863E-4	2.805804345867185E-4
37	0.003577400540980661	0.0	0.0	1.1870710694053476E-4	2.805804345867185E-4
38	0.0038094189772735244	0.0	0.0	1.2949866211694702E-4	3.0755932252774914E-4
39	0.0041601445205069225	0.0	0.0	1.3489443970515313E-4	3.237466552923675E-4
40	0.004591806727563413	0.0	0.0	1.4029021729335925E-4	3.399339880569859E-4
41	0.004866991384561925	0.0	0.0	1.4029021729335925E-4	3.45329765645192E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	46990	0.0	39.61843	1
TCAACGC	57215	0.0	32.768158	12
CAACGCA	57420	0.0	32.696186	13
AACGCAG	58220	0.0	32.35993	14
GTGGTAT	57785	0.0	32.282253	6
GGTATCA	58000	0.0	32.138275	8
ACGCAGA	58295	0.0	32.088516	15
AGTGGTA	58200	0.0	32.0561	5
AGCAGTG	58470	0.0	32.044724	2
GTATCAA	58230	0.0	32.031513	9
ATCAACG	58710	0.0	32.00179	11
TGGTATC	58680	0.0	31.709784	7
GCAGTGG	59370	0.0	31.566868	3
TATCAAC	59420	0.0	31.480984	10
CGCAGAG	59955	0.0	31.18047	16
AGAGTAC	59595	0.0	30.978437	19
CAGTGGT	60480	0.0	30.87095	4
CAGAGTA	61990	0.0	29.95217	18
GCAGAGT	63985	0.0	29.172543	17
GAGTACT	19315	0.0	28.640434	20
>>END_MODULE