FastQCFastQC Report
Thu 26 May 2016
SRR805256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8777737
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT606510.6909639694148959No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC93680.10672454642922202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT491150.042.856461
GTGGTAT517100.041.151136
TCAACGC519800.040.9780712
CAACGCA520800.040.89035813
GGTATCA521100.040.871338
GTATCAA523100.040.7195559
AGTGGTA522250.040.7048345
ACGCAGA523650.040.62293615
TGGTATC524000.040.564417
AACGCAG526050.040.4867414
ATCAACG527100.040.45067211
TATCAAC529700.040.2787410
CGCAGAG531450.040.0797816
CAGTGGT532450.039.8676874
GCAGTGG534150.039.8287933
AGAGTAC528950.039.7005419
AGCAGTG536800.039.6671942
CAGAGTA538250.039.16739318
GCAGAGT549100.038.5689317
ACATGGG395150.038.49563224
Produced by FastQC (version 0.11.5)