##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805256_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8777737
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.62996385059156	35.0	35.0	37.0	33.0	37.0
2	35.41752185101923	37.0	35.0	37.0	35.0	37.0
3	37.6909254629069	39.0	37.0	39.0	35.0	39.0
4	37.66953031288133	39.0	38.0	39.0	35.0	39.0
5	37.68652512600913	39.0	38.0	39.0	35.0	39.0
6	37.66201436657307	39.0	37.0	39.0	35.0	39.0
7	37.51394533693593	39.0	37.0	39.0	35.0	39.0
8	38.51779211430008	40.0	38.0	40.0	36.0	40.0
9	38.42880197937122	40.0	38.0	40.0	36.0	40.0
10	38.40240098330583	40.0	38.0	40.0	36.0	40.0
11	38.36679191914727	40.0	38.0	40.0	35.0	40.0
12	38.37717751169806	40.0	38.0	40.0	35.0	40.0
13	38.42716214896846	40.0	38.0	40.0	35.0	40.0
14	38.278088076687645	40.0	38.0	40.0	35.0	40.0
15	38.26010428428193	40.0	38.0	40.0	35.0	40.0
16	38.20443059526618	40.0	38.0	40.0	34.0	40.0
17	38.00736659118404	40.0	38.0	40.0	34.0	40.0
18	37.969847923217564	40.0	38.0	40.0	34.0	40.0
19	37.86504767686706	40.0	38.0	40.0	34.0	40.0
20	37.50276477866676	39.0	37.0	40.0	33.0	40.0
21	37.412617056081764	39.0	37.0	40.0	33.0	40.0
22	37.31844198567353	39.0	37.0	40.0	33.0	40.0
23	37.09602429418881	39.0	37.0	40.0	33.0	40.0
24	36.86968007813403	38.0	36.0	40.0	32.0	40.0
25	36.74150467255968	38.0	36.0	40.0	32.0	40.0
26	36.6621919749931	38.0	36.0	40.0	31.0	40.0
27	36.52327200051676	38.0	36.0	40.0	31.0	40.0
28	36.37455246152852	38.0	35.0	40.0	31.0	40.0
29	36.11537632079886	38.0	35.0	40.0	30.0	40.0
30	35.886720575018366	38.0	35.0	39.0	30.0	40.0
31	35.641230763692285	38.0	34.0	39.0	30.0	40.0
32	35.3583571711023	37.0	34.0	39.0	30.0	40.0
33	35.099245397760264	37.0	34.0	39.0	29.0	40.0
34	34.84189968325549	37.0	33.0	38.0	29.0	40.0
35	34.48784851949882	36.0	33.0	38.0	27.0	40.0
36	34.37519408476239	36.0	33.0	38.0	27.0	39.0
37	33.9359583227431	36.0	32.0	38.0	27.0	39.0
38	33.7378262757246	35.0	32.0	38.0	27.0	39.0
39	33.61032382264358	35.0	32.0	38.0	26.0	39.0
40	33.381147783306794	35.0	31.0	38.0	26.0	39.0
41	33.1081926924901	35.0	31.0	38.0	25.0	39.0
42	32.82642257338082	34.0	31.0	38.0	24.0	39.0
43	32.47402684769434	34.0	30.0	37.0	24.0	39.0
44	32.14127001071005	34.0	30.0	37.0	24.0	38.0
45	32.033637827152944	34.0	30.0	37.0	24.0	38.0
46	31.77651574659847	33.0	29.0	37.0	24.0	38.0
47	31.55756500792858	33.0	29.0	36.0	23.0	38.0
48	31.11486309056651	33.0	29.0	36.0	23.0	38.0
49	30.79308630459081	32.0	28.0	36.0	22.0	38.0
50	30.458841042970416	32.0	28.0	35.0	22.0	38.0
51	30.049427204301065	32.0	27.0	35.0	20.0	37.0
52	29.860912898164983	31.0	27.0	35.0	20.0	37.0
53	31.872990270726955	34.0	30.0	36.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	0.0
11	23.0
12	70.0
13	225.0
14	622.0
15	1293.0
16	2109.0
17	3157.0
18	4771.0
19	7533.0
20	11528.0
21	17334.0
22	25346.0
23	34781.0
24	46461.0
25	60578.0
26	77090.0
27	96040.0
28	118340.0
29	144519.0
30	178290.0
31	222809.0
32	286285.0
33	387391.0
34	580168.0
35	1058730.0
36	2296291.0
37	2630009.0
38	482937.0
39	3004.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.402890517225568	43.15341186458423	33.22675309137196	14.216944526818246
2	31.825913672282503	42.88836632949928	13.527256512697978	11.758463485520243
3	36.62049797117412	25.49813237739978	20.74499384066759	17.136375810758516
4	23.546581539182593	26.967725280445293	19.939444528811926	29.54624865156019
5	20.27186506043642	33.45948961560366	24.428722346089888	21.839922977870035
6	27.893248567369927	24.838417920245277	22.614405056793114	24.653928455591686
7	23.362160429276933	24.508913857865643	29.406463192050524	22.7224625208069
8	25.706238407461967	26.15675315858746	25.49417919447803	22.642829239472544
9	27.261798798483028	24.908458751953948	25.503521010027985	22.32622143953504
10	22.31505683070705	26.316418457285746	29.52181182917647	21.846712882830737
11	21.8393989248026	31.104702726910137	25.326117654242776	21.72978069404449
12	21.998779412051192	25.55995924689929	30.131786814756467	22.30947452629305
13	21.877062390910098	26.1752203329856	25.096343169087888	26.851374107016422
14	22.209722164152332	31.099564728357663	24.816578578282762	21.874134529207243
15	22.11042550033112	30.587325639854555	24.81630515929106	22.48594370052327
16	22.428548497180994	25.557851642171553	24.92551326156161	27.088086599085848
17	27.483860589580207	25.33741897256662	24.921799320257602	22.25692111759557
18	22.428639636844895	25.261419885330355	24.794739236320247	27.5152012415045
19	22.441137163257455	30.653948734166903	24.763136557862236	22.141777544713403
20	27.678375417263013	25.734092967242013	24.720084459126536	21.867447156368435
21	22.85302008934649	30.301602793521837	24.639494211321207	22.205882905810462
22	27.2642367844924	25.54819083779794	25.090282381438406	22.09728999627125
23	22.44199159760654	25.47924368205609	30.13487417087115	21.943890549466225
24	22.662663508829212	29.935506156085562	24.742208612538743	22.659621722546483
25	22.22804123659663	25.643249507247713	24.73470098272482	27.394008273430842
26	22.50156276042447	29.121765666936707	26.186202662485787	22.190468910153037
27	22.86576824983478	24.99545156114839	29.76515473179477	22.37362545722206
28	26.48238378525126	25.353174741963674	25.95556235052383	22.208879122261237
29	26.6327870156055	25.34866332860053	25.885943039760704	22.132606616033268
30	26.727276062155887	24.87861051202605	25.87183917677187	22.522274249046195
31	24.865133234226544	25.91493684533952	26.30992475623273	22.910005164201205
32	23.954306218106105	26.64100097781467	26.63376676699245	22.77092603708678
33	23.912461719917104	26.008161329053266	26.605513471182835	23.4738634798468
34	23.463621660115813	26.269162541552564	26.87658561654331	23.390630181788314
35	23.445348157503464	26.293496831814394	26.912608568700563	23.348546441981572
36	23.642107299409858	25.925884997465747	26.878716006187016	23.553291696937375
37	23.55837273319991	26.20045462742846	26.94062262289244	23.30055001647919
38	23.730546950768748	26.139459407362057	27.046982610666053	23.083011031203146
39	23.845234825331403	25.768931103768544	26.964364505338906	23.421469565561146
40	23.419532847703227	26.243780145155863	26.86576278145495	23.47092422568596
41	23.599693178321473	26.363423738943194	26.84697661823315	23.189906464502183
42	23.833329706734208	26.018209476998454	26.69225564630155	23.45620516996579
43	23.59992102748123	26.295182915596584	26.931747898119983	23.173148158802206
44	23.698727815608965	26.164762056552842	26.778166171987156	23.358343955851034
45	23.808801744686587	25.723965072090905	26.73269887215805	23.734534311064458
46	23.518761156776513	26.155613912788684	26.69560502894994	23.630019901484857
47	23.702521504118888	26.15341516839705	26.67878976096003	23.465273566524036
48	23.929755471142506	25.683442099028486	26.710073450594386	23.676728979234625
49	23.488480003445076	26.23774214242236	26.706860777441836	23.566917076690725
50	23.75622555107313	26.08071989397723	26.812138481706615	23.350916073243024
51	23.76822180933423	25.771904535303346	26.741539419556542	23.71833423580588
52	23.754596429580882	26.156570879259654	26.489014195800127	23.59981849535934
53	23.828966395324898	26.15611518094014	26.46289128963422	23.552027134100737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	229.0
1	190.0
2	151.0
3	157.5
4	164.0
5	195.0
6	226.0
7	266.5
8	406.5
9	506.0
10	857.0
11	1208.0
12	1827.0
13	2446.0
14	3363.5
15	4281.0
16	6665.5
17	9050.0
18	12994.0
19	16938.0
20	23561.5
21	30185.0
22	75956.0
23	121727.0
24	106431.5
25	99892.5
26	108649.0
27	123867.5
28	139086.0
29	156929.5
30	174773.0
31	188831.0
32	202889.0
33	226308.0
34	249727.0
35	279316.5
36	308906.0
37	340116.5
38	371327.0
39	400691.5
40	430056.0
41	461994.0
42	521899.0
43	549866.0
44	579834.5
45	609803.0
46	626907.0
47	644011.0
48	654690.5
49	665370.0
50	660432.5
51	655495.0
52	635144.0
53	614793.0
54	586696.0
55	558599.0
56	520504.5
57	482410.0
58	432949.0
59	337398.0
60	291308.0
61	253351.5
62	215395.0
63	182276.0
64	149157.0
65	120896.5
66	92636.0
67	73152.0
68	53668.0
69	40540.0
70	27412.0
71	20433.5
72	13455.0
73	10074.0
74	6693.0
75	4921.0
76	2301.5
77	1454.0
78	1129.5
79	805.0
80	643.0
81	481.0
82	370.0
83	259.0
84	173.0
85	87.0
86	54.0
87	21.0
88	18.0
89	15.0
90	9.5
91	4.0
92	3.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	8777737.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.04459489533507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.12010678382492	41.9000044130529
2	13.038960278713791	14.354485727963318
3	4.280361028506007	7.068322164596788
4	2.0104029015455547	4.42647253167925
5	1.1300800219855032	3.1102398504751685
6	0.7163318212874843	2.3658116948044277
7	0.4829802662073409	1.8609817167076916
8	0.35236639800408165	1.5516692506290461
9	0.2824903884401255	1.3994612094161296
>10	1.4755292150830932	14.697613696408274
>50	0.07996994917176746	2.9608497172647246
>100	0.028928604372238834	2.676862056647107
>500	0.0010535504019582864	0.38259009107237485
>1k	3.9491322133929416E-4	0.3990462824691269
>5k	2.193961728473779E-5	0.11313334013553472
>10k+	2.193961728473779E-5	0.7324562566780868
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60651	0.6909639694148959	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	9368	0.10672454642922202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4810195384072226E-4	0.0	0.0	0.0	1.1392457987747867E-5
2	8.658268070688379E-4	0.0	0.0	0.0	1.1392457987747867E-5
3	8.658268070688379E-4	0.0	0.0	0.0	1.1392457987747867E-5
4	0.0010139287609095603	0.0	0.0	0.0	3.41773739632436E-5
5	0.001025321218897308	0.0	0.0	0.0	4.5569831950991466E-5
6	0.001025321218897308	0.0	0.0	0.0	4.5569831950991466E-5
7	0.001025321218897308	0.0	0.0	0.0	4.5569831950991466E-5
8	0.001036713676885056	0.0	0.0	0.0	4.5569831950991466E-5
9	0.001036713676885056	0.0	0.0	0.0	4.5569831950991466E-5
10	0.001036713676885056	0.0	0.0	1.1392457987747867E-5	6.83547479264872E-5
11	0.0010481061348728037	0.0	0.0	1.1392457987747867E-5	1.025321218897308E-4
12	0.0010481061348728037	0.0	0.0	1.1392457987747867E-5	1.025321218897308E-4
13	0.0010822835088360474	0.0	0.0	1.1392457987747867E-5	1.025321218897308E-4
14	0.0010936759668237952	0.0	0.0	1.1392457987747867E-5	1.025321218897308E-4
15	0.0010936759668237952	1.1392457987747867E-5	0.0	1.1392457987747867E-5	1.025321218897308E-4
16	0.0010936759668237952	1.1392457987747867E-5	0.0	1.1392457987747867E-5	1.1392457987747867E-4
17	0.001116460882799291	1.1392457987747867E-5	0.0	1.1392457987747867E-5	1.2531703786522653E-4
18	0.0011392457987747867	1.1392457987747867E-5	0.0	1.1392457987747867E-5	1.2531703786522653E-4
19	0.0011506382567625347	1.1392457987747867E-5	0.0	1.1392457987747867E-5	1.2531703786522653E-4
20	0.0011506382567625347	1.1392457987747867E-5	0.0	1.1392457987747867E-5	1.9367178579171373E-4
21	0.0011620307147502825	1.1392457987747867E-5	0.0	1.1392457987747867E-5	1.9367178579171373E-4
22	0.0011848156307257781	1.1392457987747867E-5	0.0	1.1392457987747867E-5	2.050642437794616E-4
23	0.0013101326685910047	1.1392457987747867E-5	0.0	1.1392457987747867E-5	2.2784915975495733E-4
24	0.0013329175845665005	1.1392457987747867E-5	0.0	1.1392457987747867E-5	2.5063407573045306E-4
25	0.0014012723324929876	1.1392457987747867E-5	0.0	1.1392457987747867E-5	2.5063407573045306E-4
26	0.001469627080419475	1.1392457987747867E-5	0.0	1.1392457987747867E-5	2.5063407573045306E-4
27	0.0015607667443214579	1.1392457987747867E-5	0.0	1.1392457987747867E-5	2.6202653371820093E-4
28	0.0017316536141376757	1.1392457987747867E-5	0.0	1.1392457987747867E-5	3.075963656691924E-4
29	0.001800008362064163	1.1392457987747867E-5	0.0	2.2784915975495733E-5	3.075963656691924E-4
30	0.001891148025966146	1.1392457987747867E-5	0.0	2.2784915975495733E-5	3.1898882365694026E-4
31	0.001970895231880381	1.1392457987747867E-5	0.0	2.2784915975495733E-5	3.1898882365694026E-4
32	0.0021303896437088513	1.1392457987747867E-5	0.0	3.41773739632436E-5	3.3038128164468813E-4
33	0.0023012765135250694	1.1392457987747867E-5	0.0	3.41773739632436E-5	3.3038128164468813E-4
34	0.0024379860093780435	1.1392457987747867E-5	0.0	3.41773739632436E-5	3.3038128164468813E-4
35	0.0025974804212065138	1.1392457987747867E-5	1.1392457987747867E-5	3.41773739632436E-5	3.41773739632436E-4
36	0.002756974833034984	1.1392457987747867E-5	1.1392457987747867E-5	3.41773739632436E-5	3.5316619762018386E-4
37	0.002984823992789941	1.1392457987747867E-5	1.1392457987747867E-5	3.41773739632436E-5	3.6455865560793173E-4
38	0.0031215334886429157	1.1392457987747867E-5	1.1392457987747867E-5	3.41773739632436E-5	3.9873602957117533E-4
39	0.0033949524803488645	1.1392457987747867E-5	1.1392457987747867E-5	3.41773739632436E-5	4.101284875589232E-4
40	0.0036455865560793173	1.1392457987747867E-5	1.1392457987747867E-5	3.41773739632436E-5	4.101284875589232E-4
41	0.003873435715834275	1.1392457987747867E-5	1.1392457987747867E-5	3.41773739632436E-5	4.2152094554667106E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	49115	0.0	42.85646	1
GTGGTAT	51710	0.0	41.15113	6
TCAACGC	51980	0.0	40.97807	12
CAACGCA	52080	0.0	40.890358	13
GGTATCA	52110	0.0	40.87133	8
GTATCAA	52310	0.0	40.719555	9
AGTGGTA	52225	0.0	40.704834	5
ACGCAGA	52365	0.0	40.622936	15
TGGTATC	52400	0.0	40.56441	7
AACGCAG	52605	0.0	40.48674	14
ATCAACG	52710	0.0	40.450672	11
TATCAAC	52970	0.0	40.27874	10
CGCAGAG	53145	0.0	40.07978	16
CAGTGGT	53245	0.0	39.867687	4
GCAGTGG	53415	0.0	39.828793	3
AGAGTAC	52895	0.0	39.70054	19
AGCAGTG	53680	0.0	39.667194	2
CAGAGTA	53825	0.0	39.167393	18
GCAGAGT	54910	0.0	38.56893	17
ACATGGG	39515	0.0	38.495632	24
>>END_MODULE