FastQCFastQC Report
Thu 26 May 2016
SRR805255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21028384
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3511741.6699999391298923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC395500.18807912200956572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1864450.044.3341981
GTGGTAT1945250.042.647326
AGTGGTA1947600.042.4764025
TCAACGC1940900.042.417212
GTATCAA1944150.042.3620079
CAACGCA1933750.042.33706313
GGTATCA1946350.042.3201648
AGCAGTG1970400.042.1983832
ACGCAGA1948000.042.1829815
AACGCAG1957950.042.16185414
ATCAACG1956800.042.1121711
TGGTATC1956700.042.0554777
TATCAAC1959450.042.01923810
GCAGTGG1985100.041.839733
AGAGTAC1942650.041.8116519
CGCAGAG1966850.041.78228816
CAGTGGT1987700.041.6691364
CAGAGTA1968450.041.23497818
GCAGAGT1994800.040.8988117
GTACATG1344050.040.6688722