##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805255_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21028384
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.25357654682357	37.0	37.0	37.0	35.0	37.0
2	36.36173977990891	37.0	37.0	37.0	35.0	37.0
3	38.184500292556955	39.0	39.0	39.0	37.0	39.0
4	38.04772986835317	39.0	39.0	39.0	37.0	39.0
5	37.90912249842879	39.0	39.0	39.0	35.0	39.0
6	37.9641678599744	39.0	39.0	39.0	35.0	39.0
7	37.94644861916161	39.0	38.0	39.0	35.0	39.0
8	38.935938396407444	40.0	40.0	40.0	37.0	40.0
9	38.936289588396335	40.0	40.0	40.0	37.0	40.0
10	38.80127731165647	40.0	39.0	40.0	36.0	40.0
11	38.80487216706714	40.0	39.0	40.0	36.0	40.0
12	38.90551099884804	40.0	40.0	40.0	36.0	40.0
13	38.85559789092685	40.0	40.0	40.0	37.0	40.0
14	39.01805726012993	40.0	40.0	40.0	37.0	40.0
15	39.044989762408754	40.0	40.0	40.0	37.0	40.0
16	39.01896270298279	40.0	40.0	40.0	37.0	40.0
17	39.009046249107875	40.0	40.0	40.0	37.0	40.0
18	38.88280069452793	40.0	40.0	40.0	37.0	40.0
19	38.785441762904846	40.0	40.0	40.0	37.0	40.0
20	38.86711389710213	40.0	40.0	40.0	37.0	40.0
21	38.767658608478904	40.0	39.0	40.0	36.0	40.0
22	38.81289965030123	40.0	40.0	40.0	37.0	40.0
23	38.45105843606432	40.0	39.0	40.0	36.0	40.0
24	38.62706321132428	40.0	39.0	40.0	36.0	40.0
25	38.707126329821634	40.0	39.0	40.0	36.0	40.0
26	38.68556946648872	40.0	39.0	40.0	36.0	40.0
27	38.1505487059776	40.0	39.0	40.0	35.0	40.0
28	38.39125241388021	40.0	39.0	40.0	35.0	40.0
29	38.38870557052791	40.0	39.0	40.0	35.0	40.0
30	38.40088368178934	40.0	39.0	40.0	35.0	40.0
31	38.2552794356428	40.0	38.0	40.0	35.0	40.0
32	38.28627763312673	40.0	38.0	40.0	35.0	40.0
33	38.26074048295865	40.0	38.0	40.0	35.0	40.0
34	38.26012631308235	40.0	38.0	40.0	35.0	40.0
35	38.08046400522265	40.0	38.0	40.0	35.0	40.0
36	38.0221346062541	40.0	38.0	40.0	34.0	40.0
37	38.158050899203666	40.0	38.0	40.0	35.0	40.0
38	38.16397222915465	40.0	38.0	40.0	35.0	40.0
39	38.14023735727862	40.0	38.0	40.0	35.0	40.0
40	38.05376228625081	40.0	38.0	40.0	34.0	40.0
41	37.98311306280121	40.0	38.0	40.0	34.0	40.0
42	37.858149917749266	40.0	38.0	40.0	34.0	40.0
43	37.85648065015362	40.0	38.0	40.0	34.0	40.0
44	37.85725788534202	40.0	38.0	40.0	34.0	40.0
45	37.72416453874915	40.0	37.0	40.0	34.0	40.0
46	37.62234525487075	40.0	37.0	40.0	34.0	40.0
47	37.4550366780443	40.0	37.0	40.0	33.0	40.0
48	37.39064333236448	40.0	37.0	40.0	33.0	40.0
49	37.25176485268673	40.0	36.0	40.0	33.0	40.0
50	37.205307074476096	40.0	36.0	40.0	33.0	40.0
51	37.05904352897493	39.0	36.0	40.0	33.0	40.0
52	36.92799674953625	39.0	36.0	40.0	32.0	40.0
53	36.70973290196717	39.0	35.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	11.0
13	60.0
14	190.0
15	507.0
16	1235.0
17	2179.0
18	3656.0
19	5830.0
20	9270.0
21	13692.0
22	19810.0
23	26523.0
24	34721.0
25	45128.0
26	57867.0
27	73240.0
28	92289.0
29	117527.0
30	147541.0
31	187404.0
32	241545.0
33	318373.0
34	444174.0
35	664647.0
36	1073364.0
37	1978672.0
38	3809111.0
39	1.1659813E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.254141830394575	37.55547264116919	28.8573957941799	21.332989734256326
2	28.988071551289913	42.59138505364939	12.870803576727532	15.549739818333164
3	34.99942268507176	26.03451601416447	18.579810983097893	20.386250317665873
4	22.510859607661722	24.496537632183244	19.900392726326473	33.092210033828565
5	21.021496468772874	33.15435936494217	23.516253079647015	22.307891086637945
6	30.537334680591716	23.70905914596195	22.19232823596906	23.561277937477268
7	23.09473233891867	23.16434301370947	31.293698079700278	22.44722656767158
8	29.810540838516168	24.995458519304194	23.467790011824018	21.72621063035562
9	30.21436169322379	24.182490675460368	23.77943069709969	21.823716934216154
10	21.787974767818582	24.848457209075125	31.6293967239708	21.734171299135493
11	21.877254096177815	32.93205507375174	23.666188519288976	21.524502310781465
12	21.471359853424783	24.453153413976082	31.95683985987701	22.118646872722124
13	21.876536019125385	24.689386497792697	23.64152185921657	29.79255562386534
14	22.001010634007827	33.044945346252	23.39091296791993	21.563131051820246
15	21.664384671689465	32.679971984532905	23.43866271416767	22.21698062960996
16	22.084179174205683	24.200889616624842	23.6346882385256	30.080242970643866
17	30.423597933155488	24.05725042875382	23.53788098980882	21.981270648281864
18	22.073393751987787	24.256918648622737	23.487591818753167	30.182095780636303
19	22.398054933750497	32.42108380748611	23.436142311268426	21.744718947494967
20	30.5244473374654	24.470915121199994	23.526249092654954	21.47838844867965
21	22.47258752741057	32.32380101105249	23.44907245368926	21.75453900784768
22	30.07923956496134	24.06626205798791	23.972935818558383	21.88156255849237
23	22.21557776384529	24.3329872614082	31.826002416543275	21.625432558203237
24	22.036643424430522	32.09775891480772	23.649097334345807	22.216500326415954
25	21.826527421222668	24.297929883722876	23.707023801733882	30.168518893320574
26	22.186312557351055	29.714703707141737	26.324519278324004	21.774464457183203
27	22.639809126559605	24.028746098606533	31.453943393843293	21.87750138099057
28	27.902885927896314	23.98153847675599	26.226884576579923	21.888691018767776
29	28.285739883768528	24.111624554697116	25.917326790304003	21.685308771230353
30	27.964126962870754	23.90063354369028	26.096356239262132	22.03888325417683
31	25.878388943249277	24.829882315255418	26.579398588117854	22.712330153377454
32	24.311915742075094	25.962151918093184	26.996582333668627	22.7293500061631
33	24.015055079838753	25.406241392586328	27.287993219069996	23.290710308504924
34	23.551605296916776	25.237269777839323	27.712994969085592	23.498129956158305
35	23.80394518190271	25.485220357398834	27.455366993488422	23.255467467210032
36	23.814868512958483	25.35236183626854	27.46769794578604	23.365071704986935
37	23.69582940847951	25.272602973200414	27.765286196029137	23.266281422290938
38	23.94576777749541	25.32822303416183	27.63920422986379	23.086804958478975
39	23.97032506159294	25.132021557148658	27.59851161173393	23.299141769524468
40	23.62743613584382	25.395032732900447	27.506317175870482	23.471213955385252
41	23.87462583905639	25.75443267537819	27.24259267854344	23.128348807021975
42	24.03092410714965	25.5515117091261	27.267482846042757	23.15008133768149
43	23.73572786192225	25.413545805516964	27.7566740268772	23.094052305683594
44	23.87571959880512	25.363023616080056	27.346347679403227	23.414909105711594
45	23.96470408757991	25.0194784344817	27.35279135096639	23.663026126972
46	23.759296006768757	25.21745370447867	27.3016794823606	23.72157080639197
47	24.060056160283168	25.34059203027679	27.11900258241432	23.48034922702572
48	24.02863196715449	25.09023042379291	27.291726268647174	23.589411340405427
49	23.609593585508044	25.497636908285486	27.274254645530537	23.618514860675933
50	23.93923375186605	25.454262201032662	27.23976792510542	23.36673612199587
51	23.844029098955012	25.28569955732214	27.251732705661073	23.61853863806177
52	23.8531310822553	25.349717790962917	27.133112083172918	23.664039043608867
53	24.071207754243026	25.501926348691367	26.7357586774143	23.691107219651304
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6001.0
1	3679.0
2	1357.0
3	1095.0
4	833.0
5	831.5
6	830.0
7	934.0
8	1312.0
9	1586.0
10	2458.0
11	3330.0
12	4889.0
13	6448.0
14	8022.5
15	9597.0
16	14286.5
17	18976.0
18	26567.5
19	34159.0
20	49791.0
21	65423.0
22	286576.0
23	507729.0
24	362452.0
25	224610.0
26	232045.0
27	257186.0
28	282327.0
29	314160.5
30	345994.0
31	374819.0
32	403644.0
33	452193.5
34	500743.0
35	563994.5
36	627246.0
37	697147.5
38	767049.0
39	838284.5
40	909520.0
41	983541.0
42	1131906.5
43	1206251.0
44	1286059.5
45	1365868.0
46	1423974.5
47	1482081.0
48	1536935.0
49	1591789.0
50	1592738.5
51	1593688.0
52	1561332.5
53	1528977.0
54	1482494.0
55	1436011.0
56	1361416.5
57	1286822.0
58	1164608.0
59	921833.5
60	801273.0
61	706274.5
62	611276.0
63	524580.0
64	437884.0
65	359640.5
66	281397.0
67	226599.0
68	171801.0
69	132746.5
70	93692.0
71	70306.5
72	46921.0
73	35270.0
74	23619.0
75	18033.5
76	9183.0
77	5918.0
78	4562.0
79	3206.0
80	2717.0
81	2228.0
82	1745.0
83	1262.0
84	887.0
85	512.0
86	356.5
87	201.0
88	157.0
89	113.0
90	97.5
91	82.0
92	51.5
93	19.0
94	17.0
95	14.5
96	12.0
97	9.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.1028384E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.886030075973906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.5544089104975	28.438036546311885
2	14.661297382685925	11.988844914825885
3	5.4757953651835365	6.7165060197231785
4	2.6687844464743367	4.364640045793644
5	1.6120195688627308	3.2954540287790053
6	1.0778516965638525	2.644144612988951
7	0.7759683800644026	2.2208386567722544
8	0.5791955880365984	1.8944806585868605
9	0.44990054310424926	1.6555182422901598
>10	2.860871549509766	21.86210187780306
>50	0.2113583821722439	5.855426573643655
>100	0.06700930245007138	4.66451657754365
>500	0.0038528600657383024	1.0745840833632327
>1k	0.0015223917432874775	0.8926677234556861
>5k	8.181629288074085E-5	0.22135769708669634
>10k+	8.181629288072262E-5	2.2108817410322197
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	351174	1.6699999391298923	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	39550	0.18807912200956572	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7106219859785705E-4	0.0	0.0	0.0	4.7554771683834575E-6
2	0.008840432056024847	0.0	0.0	0.0	4.7554771683834575E-6
3	0.008902253259213832	0.0	0.0	0.0	4.7554771683834575E-6
4	0.009335001681536727	0.0	0.0	4.7554771683834575E-6	9.510954336766915E-6
5	0.009349268113041877	0.0	0.0	4.7554771683834575E-6	1.902190867353383E-5
6	0.009373045498883794	0.0	0.0	4.7554771683834575E-6	3.32883401786842E-5
7	0.009382556453220562	0.0	0.0	4.7554771683834575E-6	4.279929451545112E-5
8	0.009401578361894095	0.0	0.0	1.4266431505150373E-5	4.279929451545112E-5
9	0.009406333839062479	0.0	0.0	1.902190867353383E-5	6.182120318898494E-5
10	0.009425355747736012	0.0	0.0	2.8532863010300745E-5	8.084311186251877E-5
11	0.009425355747736012	4.7554771683834575E-6	0.0	2.8532863010300745E-5	1.3790883788312026E-4
12	0.0095727755399559	4.7554771683834575E-6	0.0	2.8532863010300745E-5	1.569307465566541E-4
13	0.009591797448629433	4.7554771683834575E-6	0.0	2.8532863010300745E-5	1.6168622372503754E-4
14	0.009606063880134583	4.7554771683834575E-6	0.0	2.8532863010300745E-5	1.9497456390372175E-4
15	0.009629841265976501	4.7554771683834575E-6	0.0	3.32883401786842E-5	1.9497456390372175E-4
16	0.009644107697481651	4.7554771683834575E-6	0.0	3.32883401786842E-5	2.0448551824048865E-4
17	0.009686906991997103	4.7554771683834575E-6	0.0	3.32883401786842E-5	2.1399647257725558E-4
18	0.009705928900670636	4.7554771683834575E-6	0.0	4.279929451545112E-5	2.1875194974563903E-4
19	0.009758239149522854	9.510954336766915E-6	0.0	4.7554771683834575E-5	2.2350742691402248E-4
20	0.00980579392120669	9.510954336766915E-6	0.0	5.231024885221803E-5	2.567957670927067E-4
21	0.00986285964722729	9.510954336766915E-6	0.0	5.231024885221803E-5	2.567957670927067E-4
22	0.009953213713426575	9.510954336766915E-6	0.0	5.231024885221803E-5	2.663067214294736E-4
23	0.010029301348120712	9.510954336766915E-6	0.0	5.706572602060149E-5	2.853286301030074E-4
24	0.010138677322993532	9.510954336766915E-6	0.0	5.706572602060149E-5	2.853286301030074E-4
25	0.010257564252203118	1.902190867353383E-5	0.0	6.182120318898494E-5	2.853286301030074E-4
26	0.01035742927273917	2.3777385841917288E-5	0.0	6.65766803573684E-5	2.900841072713909E-4
27	0.010514360019295824	2.8532863010300745E-5	0.0	6.65766803573684E-5	2.995950616081578E-4
28	0.010652268857178944	2.8532863010300745E-5	0.0	7.608763469413532E-5	3.043505387765413E-4
29	0.010775911263556915	2.8532863010300745E-5	0.0	8.559858903090224E-5	3.138614931133082E-4
30	0.010942352964450335	3.32883401786842E-5	0.0	8.559858903090224E-5	3.2812792461845856E-4
31	0.011099283711006989	3.32883401786842E-5	0.0	9.035406619928569E-5	3.32883401786842E-4
32	0.011294258274910712	3.32883401786842E-5	0.0	9.510954336766915E-5	3.4239435612360894E-4
33	0.011465455452972515	3.32883401786842E-5	0.0	9.510954336766915E-5	3.6141626479714275E-4
34	0.011631897153865936	3.32883401786842E-5	0.0	9.98650205360526E-5	3.7092721913390965E-4
35	0.011860160057948342	4.279929451545112E-5	0.0	1.0462049770443607E-4	3.804381734706766E-4
36	0.012121711302209433	4.7554771683834575E-5	0.0	1.0462049770443607E-4	3.804381734706766E-4
37	0.012354729683460223	4.7554771683834575E-5	0.0	1.0937597487281952E-4	3.899491278074435E-4
38	0.012606769973384545	5.231024885221803E-5	0.0	1.1413145204120298E-4	3.899491278074435E-4
39	0.012882587649150786	5.706572602060149E-5	0.0	1.1888692920958643E-4	3.899491278074435E-4
40	0.01317742723359056	5.706572602060149E-5	0.0	1.1888692920958643E-4	3.899491278074435E-4
41	0.01353408802121932	5.706572602060149E-5	0.0	1.1888692920958643E-4	3.994600821442104E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	186445	0.0	44.334198	1
GTGGTAT	194525	0.0	42.64732	6
AGTGGTA	194760	0.0	42.476402	5
TCAACGC	194090	0.0	42.4172	12
GTATCAA	194415	0.0	42.362007	9
CAACGCA	193375	0.0	42.337063	13
GGTATCA	194635	0.0	42.320164	8
AGCAGTG	197040	0.0	42.198383	2
ACGCAGA	194800	0.0	42.18298	15
AACGCAG	195795	0.0	42.161854	14
ATCAACG	195680	0.0	42.11217	11
TGGTATC	195670	0.0	42.055477	7
TATCAAC	195945	0.0	42.019238	10
GCAGTGG	198510	0.0	41.83973	3
AGAGTAC	194265	0.0	41.81165	19
CGCAGAG	196685	0.0	41.782288	16
CAGTGGT	198770	0.0	41.669136	4
CAGAGTA	196845	0.0	41.234978	18
GCAGAGT	199480	0.0	40.89881	17
GTACATG	134405	0.0	40.66887	22
>>END_MODULE