FastQCFastQC Report
Thu 26 May 2016
SRR805254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17841636
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT2896791.6236123189599878No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT331330.1857060641748324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT468150.034.72818820
AGAGTAC1458700.034.485519
GTACTTT468100.034.4328122
GTACATG965600.034.40762322
ACATGGG962100.034.28333724
AGTACAT977750.034.0966521
TACATGG974400.034.00041223
AGTACTT484400.033.48059521
CATGGGG585200.033.38345725
GAGTACA1030700.032.95818720
TACTTTT492000.032.77642423
ACTTTTT508850.031.94261624
ATGGGGG231700.030.09063326
AAGCAGT1858750.028.8398111
CTTTTTT558850.028.8163225
ATGGGGA235650.028.69509926
CAGAGTA1868300.028.24813818
CAACGCA1901450.028.1122313
TCAACGC1904200.028.08528512
ATCAACG1915400.027.93672411
Produced by FastQC (version 0.11.5)