##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805254_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17841636
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.982305546419624	37.0	36.0	37.0	35.0	37.0
2	35.97360920265384	37.0	37.0	37.0	35.0	37.0
3	37.78860464365488	39.0	38.0	39.0	35.0	39.0
4	37.785615736135405	39.0	38.0	39.0	35.0	39.0
5	37.76335073756689	39.0	38.0	39.0	35.0	39.0
6	37.76958648859331	39.0	38.0	39.0	35.0	39.0
7	37.660526870966315	39.0	37.0	39.0	35.0	39.0
8	38.629247508468396	40.0	39.0	40.0	36.0	40.0
9	38.69749982568863	40.0	39.0	40.0	36.0	40.0
10	38.25829027113881	40.0	38.0	40.0	34.0	40.0
11	38.491405272476136	40.0	38.0	40.0	36.0	40.0
12	38.61900298829099	40.0	39.0	40.0	36.0	40.0
13	38.728912584025366	40.0	39.0	40.0	36.0	40.0
14	38.498167040287115	40.0	39.0	40.0	36.0	40.0
15	38.5841940727857	40.0	39.0	40.0	36.0	40.0
16	38.63419649408832	40.0	39.0	40.0	36.0	40.0
17	38.604532118018774	40.0	39.0	40.0	36.0	40.0
18	38.56466895748798	40.0	39.0	40.0	36.0	40.0
19	38.49870230510251	40.0	39.0	40.0	35.0	40.0
20	38.4013912177112	40.0	39.0	40.0	35.0	40.0
21	38.32564491283198	40.0	38.0	40.0	35.0	40.0
22	38.31543620775584	40.0	39.0	40.0	35.0	40.0
23	37.97326315815433	40.0	38.0	40.0	34.0	40.0
24	38.100110494351526	40.0	38.0	40.0	34.0	40.0
25	38.16490320730677	40.0	38.0	40.0	34.0	40.0
26	38.175884823566626	40.0	38.0	40.0	34.0	40.0
27	37.99452398871942	40.0	38.0	40.0	34.0	40.0
28	37.91969206187146	40.0	38.0	40.0	34.0	40.0
29	37.81228694498643	40.0	38.0	40.0	33.0	40.0
30	37.7405474475547	40.0	38.0	40.0	33.0	40.0
31	37.52460699231842	40.0	38.0	40.0	33.0	40.0
32	37.50187578089812	40.0	38.0	40.0	32.0	40.0
33	37.56005284492969	40.0	38.0	40.0	33.0	40.0
34	37.5866008027515	40.0	38.0	40.0	33.0	40.0
35	37.54514160024338	40.0	38.0	40.0	33.0	40.0
36	37.65677093737368	40.0	38.0	40.0	33.0	40.0
37	37.60005097066211	40.0	38.0	40.0	33.0	40.0
38	37.459843256526476	40.0	37.0	40.0	33.0	40.0
39	37.3632047531964	40.0	37.0	40.0	33.0	40.0
40	36.80742405012634	40.0	37.0	40.0	30.0	40.0
41	36.842962831435415	40.0	36.0	40.0	30.0	40.0
42	36.98472976357101	40.0	36.0	40.0	31.0	40.0
43	34.62192379667425	39.0	34.0	40.0	20.0	40.0
44	35.69556990177358	39.0	35.0	40.0	26.0	40.0
45	31.082186913800953	37.0	26.0	40.0	9.0	40.0
46	33.00367572794333	37.0	30.0	40.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	5.0
10	12.0
11	31.0
12	76.0
13	152.0
14	299.0
15	639.0
16	1279.0
17	2182.0
18	3498.0
19	5262.0
20	8538.0
21	14076.0
22	22227.0
23	34084.0
24	49769.0
25	68520.0
26	92252.0
27	119901.0
28	151665.0
29	187198.0
30	228683.0
31	278290.0
32	337230.0
33	415469.0
34	533688.0
35	742016.0
36	1157426.0
37	2088187.0
38	3738825.0
39	7560156.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.90986790673232	34.05920847168948	29.510354319525405	22.52056930205279
2	26.31500272732837	36.93014474681582	18.376201599449736	18.378650926406078
3	31.544607232206733	25.35504031132571	21.056566785691626	22.043785670775932
4	21.94387891334629	24.369177804098236	22.3699609161402	31.316982366415274
5	22.333764683911273	32.03611484955752	23.972997767693503	21.657122698837707
6	30.052709291905742	24.32450140783054	23.02133055511277	22.60145874515095
7	22.992869039588072	23.799796162190507	31.50008777221999	21.70724702600143
8	29.33733767463925	25.243912609807754	23.972487724780393	21.446261990772594
9	29.200169760217054	24.42604478647586	24.502590457511854	21.87119499579523
10	21.98461508798857	24.96780003806826	31.44964957249436	21.597935301448814
11	21.657980243515784	32.4004144014596	24.378868619447232	21.56273673557739
12	21.3552781818887	24.845927806172035	31.64144252242339	22.15735148951587
13	21.548382670737144	24.998340959315616	24.347178700428593	29.10609766951865
14	22.419390239773975	32.17360784627598	23.864224110389877	21.542777803560167
15	21.523956659579873	32.248550525299365	24.07180036628928	22.155692448831484
16	22.00961840046507	24.608107686985655	24.207051416136952	29.175222496412324
17	29.49505863699943	24.382326822495425	24.220749711517485	21.901864828987655
18	21.825958112809836	24.664851362285386	24.122188122210318	29.387002402694463
19	22.14665179807502	31.961951247071735	24.206513348887963	21.684883605965283
20	29.56564072935912	24.66135953003413	24.292912376421086	21.48008736418566
21	22.003856597007136	31.960734990894334	24.153194247433362	21.882214164665168
22	29.285548701923968	24.457129379839383	24.5518572399975	21.705464678239146
23	21.95719047289161	24.614648566981188	31.550553996281504	21.877606963845693
24	21.721444154560714	31.761364260542024	24.409303048218224	22.107888536679035
25	22.008497427029674	24.946551986600333	24.208637593548037	28.83631299282196
26	22.298257850345113	29.256493070478513	26.562121321161353	21.883127758015018
27	22.388131895527966	24.357020847191368	31.219384814262547	22.035462443018115
28	27.06858272414032	24.609475274576837	26.471927798549416	21.850014202733426
29	27.281074448553934	24.493062183310993	26.401132721236998	21.82473064689808
30	27.038764830758794	24.350048392423208	26.64002897492136	21.97115780189664
31	25.625688137567654	25.27167912180251	26.7566382365384	22.34599450409144
32	23.980648411390078	25.975885843652453	27.266995022205364	22.77647072275211
33	23.48712304185558	25.667685407324754	27.78966570106015	23.05552584975952
34	23.316628587199066	25.81512143841518	27.857288423550397	23.01096155083536
35	23.12266094880537	25.63869142941824	28.00088512062459	23.237762501151803
36	23.192122067729663	25.71196946289006	27.729217208556435	23.36669126082384
37	23.304449210823492	25.744449668180653	27.772015974319842	23.179085146676012
38	23.390293356506096	25.923104809446844	27.628968554228994	23.057633279818063
39	23.58645810283317	25.45071539403673	27.567376668821176	23.395449834308916
40	23.827786868872337	25.35678902988493	27.562018415799987	23.253405685442747
41	23.627300769951816	25.683563996037133	27.32479801740154	23.364337216609506
42	23.578504796309037	25.85099819321502	27.52831074459764	23.04218626587831
43	25.449302967508135	26.069935514882154	26.744901644669806	21.7358598729399
44	23.455057596736083	25.856059388275828	27.38991536426368	23.298967650724407
45	24.393127401545463	29.504149731560492	25.200732713076313	20.90199015381773
46	23.28458556154828	25.49848567698612	27.695386230276192	23.521542531189404
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3313.0
1	2278.0
2	1243.0
3	1073.0
4	903.0
5	951.5
6	1000.0
7	1000.0
8	1431.0
9	1862.0
10	2873.0
11	3884.0
12	6341.0
13	8798.0
14	12323.0
15	15848.0
16	22996.5
17	30145.0
18	44162.5
19	58180.0
20	58180.0
21	78555.5
22	98931.0
23	129567.5
24	160204.0
25	358914.5
26	557625.0
27	450900.5
28	344176.0
29	364550.0
30	384924.0
31	384924.0
32	431356.0
33	477788.0
34	536286.0
35	594784.0
36	664153.0
37	733522.0
38	802077.0
39	870632.0
40	946344.0
41	1022056.0
42	1111831.0
43	1201606.0
44	1201606.0
45	1308577.0
46	1415548.0
47	1572478.0
48	1729408.0
49	1636541.5
50	1543675.0
51	1479773.5
52	1415872.0
53	1353417.0
54	1290962.0
55	1199135.5
56	1107309.0
57	1107309.0
58	997471.5
59	887634.0
60	780763.0
61	673892.0
62	576864.5
63	479837.0
64	398812.5
65	317788.0
66	257882.0
67	197976.0
68	153341.0
69	108706.0
70	108706.0
71	81132.0
72	53558.0
73	39622.0
74	25686.0
75	18859.5
76	12033.0
77	8727.0
78	5421.0
79	4056.0
80	2691.0
81	2691.0
82	1989.0
83	1287.0
84	908.0
85	529.0
86	406.0
87	283.0
88	175.5
89	68.0
90	49.0
91	30.0
92	20.0
93	10.0
94	10.0
95	8.0
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.7841636E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.35379115983658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.39017207347845	34.01912709478491
2	13.173694641017478	12.21301380386365
3	4.700303415636617	6.53630648748859
4	2.3639466700534024	4.383115610265861
5	1.3629599884645254	3.1589181332248946
6	0.9062936364052396	2.520608757085041
7	0.6597110368800257	2.140607534056317
8	0.49940014010468176	1.8519271839684441
9	0.4043168928129619	1.686745873066146
>10	2.3542200784430167	19.990972420929015
>50	0.1404017714254165	4.377480254266775
>100	0.04259957985355375	3.3652613675062755
>500	0.0010082224327386843	0.30073185185785806
>1k	6.88954155400898E-4	0.8000155059965599
>5k	2.459989882144356E-4	0.7254522503458276
>10k+	3.6899848232165287E-5	1.9297158712937683
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	289679	1.6236123189599878	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	33133	0.1857060641748324	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8496061684029425E-4	0.0	0.0	0.0	5.6048671769786134E-6
2	2.4100928861008037E-4	5.6048671769786134E-6	0.0	0.0	5.6048671769786134E-6
3	2.46614155787059E-4	5.6048671769786134E-6	1.1209734353957227E-5	0.0	5.6048671769786134E-6
4	2.522190229640376E-4	5.6048671769786134E-6	1.1209734353957227E-5	0.0	1.1209734353957227E-5
5	2.522190229640376E-4	5.6048671769786134E-6	1.1209734353957227E-5	0.0	3.362920306187168E-5
6	2.578238901410162E-4	5.6048671769786134E-6	1.1209734353957227E-5	0.0	4.483893741582891E-5
7	2.578238901410162E-4	5.6048671769786134E-6	1.1209734353957227E-5	0.0	4.483893741582891E-5
8	2.6342875731799484E-4	5.6048671769786134E-6	1.1209734353957227E-5	0.0	4.483893741582891E-5
9	2.6342875731799484E-4	1.681460153093584E-5	1.1209734353957227E-5	0.0	5.6048671769786136E-5
10	2.6342875731799484E-4	1.681460153093584E-5	1.1209734353957227E-5	0.0	6.725840612374336E-5
11	2.6342875731799484E-4	3.362920306187168E-5	1.1209734353957227E-5	0.0	8.40730076546792E-5
12	2.6903362449497343E-4	3.362920306187168E-5	1.1209734353957227E-5	5.6048671769786134E-6	8.967787483165781E-5
13	2.746384916719521E-4	3.362920306187168E-5	1.1209734353957227E-5	5.6048671769786134E-6	1.0088760918561504E-4
14	2.746384916719521E-4	3.362920306187168E-5	1.1209734353957227E-5	1.1209734353957227E-5	1.233070778935295E-4
15	3.0266282755684514E-4	3.362920306187168E-5	1.1209734353957227E-5	1.1209734353957227E-5	1.289119450705081E-4
16	3.306871634417382E-4	3.362920306187168E-5	1.1209734353957227E-5	1.1209734353957227E-5	1.6254114813237978E-4
17	3.867358352115243E-4	3.362920306187168E-5	1.1209734353957227E-5	1.1209734353957227E-5	1.73750882486337E-4
18	4.20365038273396E-4	3.362920306187168E-5	1.1209734353957227E-5	1.1209734353957227E-5	1.7935574966331563E-4
19	4.652039756892249E-4	3.362920306187168E-5	1.1209734353957227E-5	1.1209734353957227E-5	1.9617035119425146E-4
20	4.988331787510966E-4	3.362920306187168E-5	1.1209734353957227E-5	1.1209734353957227E-5	2.2419468707914454E-4
21	5.885110535827544E-4	3.362920306187168E-5	1.1209734353957227E-5	1.1209734353957227E-5	2.2979955425612316E-4
22	7.286327330072197E-4	3.362920306187168E-5	1.1209734353957227E-5	1.681460153093584E-5	2.46614155787059E-4
23	9.248030842014712E-4	3.362920306187168E-5	1.1209734353957227E-5	1.681460153093584E-5	2.8024335884893067E-4
24	0.001059319896448958	3.362920306187168E-5	1.1209734353957227E-5	1.681460153093584E-5	2.970579603798665E-4
25	0.0012386756461122736	3.362920306187168E-5	1.1209734353957227E-5	1.681460153093584E-5	3.0266282755684514E-4
26	0.0014572654660144394	3.9234070238850297E-5	1.1209734353957227E-5	1.681460153093584E-5	3.0826769473382373E-4
27	0.0016702504187396268	3.9234070238850297E-5	1.1209734353957227E-5	1.681460153093584E-5	3.0826769473382373E-4
28	0.0019112597073497072	4.483893741582891E-5	1.1209734353957227E-5	1.681460153093584E-5	3.2508229626475955E-4
29	0.0022139225349065523	5.044380459280752E-5	1.1209734353957227E-5	3.9234070238850297E-5	3.2508229626475955E-4
30	0.0023988831517468465	5.044380459280752E-5	1.1209734353957227E-5	4.483893741582891E-5	3.362920306187168E-4
31	0.0026735216434187986	5.044380459280752E-5	1.1209734353957227E-5	4.483893741582891E-5	3.4189689779569543E-4
32	0.0029817893381526225	5.044380459280752E-5	1.1209734353957227E-5	7.286327330072198E-5	3.5871149932663126E-4
33	0.0033012667672404033	6.165353894676475E-5	1.1209734353957227E-5	7.286327330072198E-5	3.6431636650360985E-4
34	0.003609534461974227	6.165353894676475E-5	1.1209734353957227E-5	1.1770221071655088E-4	3.699212336805885E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACT	46815	0.0	34.728188	20
AGAGTAC	145870	0.0	34.4855	19
GTACTTT	46810	0.0	34.43281	22
GTACATG	96560	0.0	34.407623	22
ACATGGG	96210	0.0	34.283337	24
AGTACAT	97775	0.0	34.09665	21
TACATGG	97440	0.0	34.000412	23
AGTACTT	48440	0.0	33.480595	21
CATGGGG	58520	0.0	33.383457	25
GAGTACA	103070	0.0	32.958187	20
TACTTTT	49200	0.0	32.776424	23
ACTTTTT	50885	0.0	31.942616	24
ATGGGGG	23170	0.0	30.090633	26
AAGCAGT	185875	0.0	28.839811	1
CTTTTTT	55885	0.0	28.81632	25
ATGGGGA	23565	0.0	28.695099	26
CAGAGTA	186830	0.0	28.248138	18
CAACGCA	190145	0.0	28.11223	13
TCAACGC	190420	0.0	28.085285	12
ATCAACG	191540	0.0	27.936724	11
>>END_MODULE