FastQCFastQC Report
Thu 26 May 2016
SRR805253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31709629
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1962400.618865644880298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1012000.035.5711481
TCAACGC1179750.030.65225612
CAACGCA1185350.030.49732213
AGCAGTG1196450.030.1007162
ATCAACG1202650.030.10019511
ACGCAGA1204200.030.04484215
AACGCAG1207300.029.9759814
GCAGTGG1203450.029.9472373
CGCAGAG1226450.029.51119216
AGAGTAC1224400.029.21757519
GGTATCA1059500.029.058998
GTATCAA1064250.028.9255359
GTGGTAT1063850.028.9100916
AGTGGTA1067150.028.8300615
TGGTATC1069200.028.759827
TATCAAC1081600.028.54474810
CAGAGTA1263200.028.45788418
GCAGAGT1280500.028.22959717
CAGTGGT1103500.027.8622574
GAGTACT456950.027.84549720
Produced by FastQC (version 0.11.5)