##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805253_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31709629
Sequences flagged as poor quality	0
Sequence length	46
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.37611345121698	37.0	37.0	37.0	35.0	37.0
2	36.258484260411876	37.0	37.0	37.0	35.0	37.0
3	38.14150146632116	39.0	39.0	39.0	37.0	39.0
4	38.10794626452426	39.0	39.0	39.0	37.0	39.0
5	38.10950374096146	39.0	39.0	39.0	37.0	39.0
6	37.94506586627046	39.0	38.0	39.0	35.0	39.0
7	37.94707544512741	39.0	38.0	39.0	35.0	39.0
8	38.84990221739901	40.0	39.0	40.0	36.0	40.0
9	38.6964853483464	40.0	39.0	40.0	36.0	40.0
10	33.599808247520016	40.0	36.0	40.0	3.0	40.0
11	35.400173461505965	40.0	36.0	40.0	17.0	40.0
12	37.38687800478523	40.0	36.0	40.0	32.0	40.0
13	38.12852998059359	40.0	37.0	40.0	34.0	40.0
14	38.23978356858101	40.0	38.0	40.0	35.0	40.0
15	38.293812582922364	40.0	38.0	40.0	35.0	40.0
16	38.26359456933413	40.0	38.0	40.0	35.0	40.0
17	38.081981186219494	40.0	38.0	40.0	34.0	40.0
18	37.97119654096237	40.0	38.0	40.0	34.0	40.0
19	37.856093901319376	39.0	38.0	40.0	34.0	40.0
20	37.57218786760325	39.0	37.0	40.0	34.0	40.0
21	37.39095834265358	39.0	37.0	40.0	33.0	40.0
22	37.23462983436356	38.0	36.0	40.0	33.0	40.0
23	37.07181414831438	38.0	36.0	40.0	33.0	40.0
24	36.806202305299756	38.0	36.0	40.0	32.0	40.0
25	36.542028006697905	38.0	36.0	40.0	32.0	40.0
26	36.365000549202264	38.0	35.0	39.0	31.0	40.0
27	35.60159682095303	38.0	34.0	39.0	30.0	40.0
28	35.36125162486133	38.0	34.0	39.0	30.0	40.0
29	35.05919870585682	37.0	34.0	38.0	29.0	40.0
30	34.88134692462028	37.0	34.0	38.0	29.0	40.0
31	34.6866864951337	37.0	33.0	38.0	29.0	40.0
32	34.413784752890045	36.0	33.0	38.0	28.0	39.0
33	34.21856960231228	36.0	33.0	38.0	27.0	39.0
34	33.99256156544752	36.0	32.0	38.0	27.0	39.0
35	33.69263755813731	35.0	32.0	38.0	27.0	39.0
36	33.56419083301164	35.0	31.0	38.0	27.0	39.0
37	32.9678382235251	35.0	31.0	38.0	25.0	38.0
38	32.36759348398557	34.0	30.0	37.0	24.0	38.0
39	32.236828346367595	34.0	30.0	37.0	24.0	38.0
40	31.920393045279717	34.0	30.0	37.0	24.0	38.0
41	31.544342634850757	33.0	29.0	37.0	23.0	38.0
42	31.259359136620613	33.0	29.0	36.0	23.0	38.0
43	27.684610091149285	31.0	23.0	35.0	10.0	37.0
44	28.987980370252835	31.0	25.0	35.0	15.0	37.0
45	26.166977355679563	30.0	19.0	34.0	9.0	37.0
46	28.25445267745012	31.0	24.0	34.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	11.0
12	57.0
13	256.0
14	1159.0
15	2639.0
16	4446.0
17	5891.0
18	7685.0
19	11149.0
20	20066.0
21	35970.0
22	61913.0
23	98879.0
24	146847.0
25	206119.0
26	275681.0
27	354313.0
28	444601.0
29	546626.0
30	671617.0
31	861206.0
32	1243054.0
33	2121076.0
34	3091884.0
35	4014890.0
36	7509607.0
37	8395567.0
38	1544655.0
39	31762.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.187822884966582	30.50292073742017	20.20272769511116	35.10652868250209
2	23.591118016549483	26.861064820405183	28.33814296597415	21.20967419707118
3	18.676323838415136	24.25700092549175	31.544582246610325	25.522092989482786
4	17.254515970527436	27.461220060316695	30.69621848934278	24.588045479813086
5	22.385900509905053	29.254511303175452	28.83319763848388	19.526390548435618
6	23.531158311565235	25.712435803017435	29.800004913334057	20.956400972083276
7	22.889123048396435	26.818765366192082	29.962047175007946	20.33006441040354
8	23.214204114466302	25.349170751887385	29.02562183871656	22.411003294929753
9	23.327664918438497	24.683858647478974	30.278805847901914	21.70967058618062
10	20.42498195106603	34.089919500477286	26.059898083323525	19.42520046513316
11	21.44611972596715	29.020705351046523	27.453752297133466	22.07942262585286
12	21.5529358605867	26.22974554511502	29.71447884174236	22.50283975255592
13	21.843323994739894	26.76978970646424	26.36028002724346	25.026606271552403
14	22.169269151651065	29.4774498938477	26.326397574692535	22.0268833798087
15	21.850492164383255	29.05630337081522	26.42016404543869	22.673040419362838
16	22.428663545700896	26.33324722909877	26.1825043743022	25.05558485089813
17	25.26519310585438	26.0256025070492	26.222268321083163	22.48693606601326
18	22.028545335550913	26.461826469177545	26.250565719327717	25.25906247594382
19	22.716279651206264	28.89765124656615	26.059648947642998	22.32642015458459
20	25.19383623189032	26.341137576854024	26.2310164524473	22.23400973880836
21	22.564205970369443	28.861980693624638	26.343546939637797	22.23026639636812
22	24.966277593471688	25.992325548810424	26.49344462529032	22.54795223242757
23	22.257816387571104	26.42493862037932	29.208468506522106	22.10877648552747
24	22.13123654016892	28.785805094093025	26.393083943050865	22.68987442268719
25	22.213596381086642	26.19270632273875	26.28144592924755	25.312251366927065
26	22.359356522272776	27.702206796553817	27.788480275187073	22.14995640598633
27	22.574786352751083	25.765703534405905	29.33062383038288	22.328886282460132
28	24.349537485916343	25.88792823782328	27.244765304570418	22.517768971689954
29	24.41860798812878	25.95228723741927	27.348285910251423	22.280818864200526
30	24.168324391307134	25.867275205269667	27.41118163192638	22.553218771496823
31	23.432806482851	26.25491455608011	27.349418058470505	22.962860902598386
32	23.11586805383311	26.70700751497282	27.531142038905593	22.645982392288474
33	22.90227362798852	26.432037410466076	27.69572296162784	22.969965999917562
34	22.944702380466197	26.425317054324417	27.52905119135894	23.100929373850448
35	22.943478777377056	26.542855484055018	27.60839932879694	22.905266409770988
36	22.86043775535816	26.389605504372188	27.703707917869362	23.046248822400287
37	23.081402182283494	26.32164507506537	27.35979030218234	23.237162440468794
38	23.32630886346857	26.488982258354394	27.531580391558663	22.65312848661837
39	22.980991042184694	26.350024467331355	27.670339504760527	22.99864498572342
40	22.96499590077197	26.496809533785466	27.438403647043614	23.099790918398952
41	23.23561401491011	26.57096681894323	27.506436609523245	22.686982556623416
42	22.89252264666988	26.398716301600377	27.688293041839117	23.020468009890624
43	24.389039682551946	26.787847312877737	26.871496982824993	21.95161602174532
44	23.011647345353676	26.33272688242426	27.547695370387338	23.10793040183472
45	22.927663392088252	29.630340361282688	25.918332251695535	21.523663994933525
46	22.948748470062515	26.329923317614345	27.39709442832018	23.32423378400296
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6218.0
1	5310.0
2	4402.0
3	3852.0
4	3302.0
5	3432.5
6	3563.0
7	3563.0
8	4725.5
9	5888.0
10	8680.5
11	11473.0
12	18607.0
13	25741.0
14	37493.5
15	49246.0
16	69546.0
17	89846.0
18	125785.0
19	161724.0
20	161724.0
21	214812.0
22	267900.0
23	340001.0
24	412102.0
25	617805.5
26	823509.0
27	799307.5
28	775106.0
29	848066.5
30	921027.0
31	921027.0
32	1027226.5
33	1133426.0
34	1250433.0
35	1367440.0
36	1496628.0
37	1625816.0
38	1750729.5
39	1875643.0
40	1996850.0
41	2118057.0
42	2235167.0
43	2352277.0
44	2352277.0
45	2426519.5
46	2500762.0
47	2529845.5
48	2558929.0
49	2524020.0
50	2489111.0
51	2400557.5
52	2312004.0
53	2184446.5
54	2056889.0
55	1892008.0
56	1727127.0
57	1727127.0
58	1537843.0
59	1348559.0
60	1172150.0
61	995741.0
62	848719.5
63	701698.0
64	579327.0
65	456956.0
66	364309.5
67	271663.0
68	205680.5
69	139698.0
70	139698.0
71	102227.0
72	64756.0
73	46863.5
74	28971.0
75	20920.0
76	12869.0
77	9242.0
78	5615.0
79	4128.5
80	2642.0
81	2642.0
82	1956.0
83	1270.0
84	836.0
85	402.0
86	319.0
87	236.0
88	127.0
89	18.0
90	12.0
91	6.0
92	3.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	3.1709629E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.74027268872294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.2103702761348	26.974285398710478
2	16.873760559405422	13.748832129487898
3	6.302602084072858	7.703091826609251
4	3.1199076465979236	5.08423553144125
5	1.7256523152328083	3.5151772944255346
6	1.0966637462007074	2.6807028040831953
7	0.7960156588020647	2.2700926502862693
8	0.5597712868518373	1.8244187895728963
9	0.4328202711662966	1.5869894285269737
>10	2.5643570412289742	19.976780856444897
>50	0.22260874059050134	6.259399048889798
>100	0.09164232297470529	6.230891017241458
>500	0.0030487470741484123	0.8151822225528351
>1k	7.062851599069195E-4	0.4182656678039377
>5k	4.8679004859837324E-5	0.12164593305888116
>10k+	2.43395023049503E-5	0.7900094008643795
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	196240	0.618865644880298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.870818734586898E-4	0.0	0.0	3.468977830046514E-5	1.5768081045665972E-5
2	0.0010123108031317553	0.0	0.0	3.468977830046514E-5	1.5768081045665972E-5
3	0.0010343861165956877	9.460848627399583E-6	3.1536162091331942E-6	3.468977830046514E-5	2.207531346393236E-5
4	0.0010627686624778865	1.2614464836532777E-5	3.1536162091331942E-6	3.784339450959833E-5	3.784339450959833E-5
5	0.0027467997181550123	1.2614464836532777E-5	3.1536162091331942E-6	3.784339450959833E-5	4.415062692786472E-5
6	0.0028161792747559426	1.8921697254799166E-5	3.1536162091331942E-6	3.784339450959833E-5	5.991870797353069E-5
7	0.0028193328909650755	1.8921697254799166E-5	3.1536162091331942E-6	4.415062692786472E-5	5.991870797353069E-5
8	0.0028287937395924754	2.207531346393236E-5	3.1536162091331942E-6	4.415062692786472E-5	6.307232418266389E-5
9	0.0028319473558016083	2.5228929673065554E-5	3.1536162091331942E-6	4.415062692786472E-5	6.622594039179708E-5
10	0.002854022669265541	2.8382545882198748E-5	3.1536162091331942E-6	4.7304243136997914E-5	6.937955660093028E-5
11	0.0030274715607678665	2.8382545882198748E-5	3.1536162091331942E-6	4.7304243136997914E-5	7.253317281006346E-5
12	0.0031094655822053296	3.468977830046514E-5	3.1536162091331942E-6	5.045785934613111E-5	9.145487006486263E-5
13	0.003383830192399917	3.784339450959833E-5	3.1536162091331942E-6	5.045785934613111E-5	1.0091571869226222E-4
14	0.0034311344355369153	3.784339450959833E-5	3.1536162091331942E-6	5.36114755552643E-5	1.040693349013954E-4
15	0.00355412546769311	3.784339450959833E-5	3.1536162091331942E-6	5.36114755552643E-5	1.103765673196618E-4
16	0.003569893548738776	3.784339450959833E-5	3.1536162091331942E-6	5.991870797353069E-5	1.1983741594706138E-4
17	0.0036108905594575075	3.784339450959833E-5	3.1536162091331942E-6	6.937955660093028E-5	1.5452719424752652E-4
18	0.0036329658729214397	3.784339450959833E-5	3.1536162091331942E-6	6.937955660093028E-5	1.576808104566597E-4
19	0.003658194802594505	3.784339450959833E-5	3.1536162091331942E-6	7.253317281006346E-5	1.671416590840593E-4
20	0.003737035207822835	4.0997010718731526E-5	3.1536162091331942E-6	7.253317281006346E-5	1.734488915023257E-4
21	0.0037938002995872326	4.415062692786472E-5	3.1536162091331942E-6	7.568678901919667E-5	1.7660250771145888E-4
22	0.0038442581589333636	4.415062692786472E-5	3.1536162091331942E-6	7.884040522832985E-5	1.8606335633885847E-4
23	0.003910484099325161	4.415062692786472E-5	3.1536162091331942E-6	7.884040522832985E-5	1.9237058875712484E-4
24	0.003983017272135224	5.36114755552643E-5	3.1536162091331942E-6	7.884040522832985E-5	1.9552420496625803E-4
25	0.004055550444945288	5.36114755552643E-5	3.1536162091331942E-6	7.884040522832985E-5	2.0183143738452443E-4
26	0.004115469152918818	5.36114755552643E-5	3.1536162091331942E-6	9.145487006486263E-5	2.081386698027908E-4
27	0.004197463174356281	5.36114755552643E-5	3.1536162091331942E-6	9.460848627399583E-5	2.081386698027908E-4
28	0.00430468612546681	5.36114755552643E-5	3.1536162091331942E-6	9.460848627399583E-5	2.081386698027908E-4
29	0.004424523541413872	5.991870797353069E-5	3.1536162091331942E-6	9.776210248312902E-5	2.081386698027908E-4
30	0.004509671179060468	5.991870797353069E-5	3.1536162091331942E-6	1.0091571869226222E-4	2.175995184301904E-4
31	0.004620047746380129	5.991870797353069E-5	3.1536162091331942E-6	1.040693349013954E-4	2.175995184301904E-4
32	0.0047461923947454574	5.991870797353069E-5	3.1536162091331942E-6	1.2614464836532778E-4	2.3336759947585638E-4
33	0.005001635307685246	5.991870797353069E-5	3.1536162091331942E-6	1.2929826457446097E-4	2.4598206431238913E-4
34	0.005130933572259707	5.991870797353069E-5	3.1536162091331942E-6	1.4191272941099374E-4	2.4598206431238913E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	101200	0.0	35.571148	1
TCAACGC	117975	0.0	30.652256	12
CAACGCA	118535	0.0	30.497322	13
AGCAGTG	119645	0.0	30.100716	2
ATCAACG	120265	0.0	30.100195	11
ACGCAGA	120420	0.0	30.044842	15
AACGCAG	120730	0.0	29.97598	14
GCAGTGG	120345	0.0	29.947237	3
CGCAGAG	122645	0.0	29.511192	16
AGAGTAC	122440	0.0	29.217575	19
GGTATCA	105950	0.0	29.05899	8
GTATCAA	106425	0.0	28.925535	9
GTGGTAT	106385	0.0	28.910091	6
AGTGGTA	106715	0.0	28.830061	5
TGGTATC	106920	0.0	28.75982	7
TATCAAC	108160	0.0	28.544748	10
CAGAGTA	126320	0.0	28.457884	18
GCAGAGT	128050	0.0	28.229597	17
CAGTGGT	110350	0.0	27.862257	4
GAGTACT	45695	0.0	27.845497	20
>>END_MODULE