FastQCFastQC Report
Thu 26 May 2016
SRR805252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40004990
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1966030.4914461920875371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1130700.034.9571041
TCAACGC1330500.029.73468612
CAACGCA1336450.029.60230613
AACGCAG1358850.029.14081814
ACGCAGA1357300.029.14020715
ATCAACG1364150.029.04959111
AGCAGTG1373600.028.782762
GCAGTGG1375650.028.7166063
CGCAGAG1385800.028.55390416
AGTGGTA1170050.028.386825
GTGGTAT1173250.028.3076926
AGAGTAC1385300.028.21049519
TGGTATC1183950.028.0366577
GGTATCA1187800.027.9811448
GTATCAA1193600.027.8300919
CAGAGTA1431550.027.42761418
TATCAAC1214150.027.423310
CAGTGGT1215650.027.2858144
GCAGAGT1458300.027.08770417
GAGTACT489600.027.04248220
Produced by FastQC (version 0.11.5)