##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805252_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40004990
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.89574748050181	37.0	35.0	37.0	35.0	37.0
2	35.94670029913768	37.0	36.0	37.0	35.0	37.0
3	37.76782978823392	39.0	38.0	39.0	35.0	39.0
4	37.793473389194695	39.0	38.0	39.0	35.0	39.0
5	37.82143225132665	39.0	38.0	39.0	35.0	39.0
6	37.68015720038925	39.0	38.0	39.0	35.0	39.0
7	37.69148621209504	39.0	38.0	39.0	35.0	39.0
8	38.55937074349975	40.0	39.0	40.0	36.0	40.0
9	38.41532958763394	40.0	38.0	40.0	35.0	40.0
10	32.90350171316128	40.0	35.0	40.0	3.0	40.0
11	34.852064654934296	40.0	35.0	40.0	17.0	40.0
12	36.98137619831926	40.0	35.0	40.0	32.0	40.0
13	37.78698614847798	40.0	37.0	40.0	34.0	40.0
14	37.92468414565283	40.0	38.0	40.0	34.0	40.0
15	37.96334222305767	40.0	38.0	40.0	34.0	40.0
16	37.93526587558202	40.0	38.0	40.0	34.0	40.0
17	37.73880208443997	40.0	37.0	40.0	34.0	40.0
18	37.653686852565144	39.0	37.0	40.0	33.0	40.0
19	37.50472156098527	39.0	37.0	40.0	33.0	40.0
20	37.22400433045978	39.0	37.0	40.0	33.0	40.0
21	37.00937775512505	38.0	36.0	40.0	32.0	40.0
22	36.88314895216822	38.0	36.0	40.0	32.0	40.0
23	36.7173241138168	38.0	36.0	40.0	32.0	40.0
24	36.44273944325445	38.0	36.0	40.0	31.0	40.0
25	36.16734087422594	38.0	35.0	40.0	31.0	40.0
26	36.00752318648249	38.0	35.0	39.0	30.0	40.0
27	35.44351719622977	38.0	34.0	39.0	29.0	40.0
28	35.125121741062806	37.0	34.0	39.0	29.0	40.0
29	34.85499501437196	37.0	34.0	38.0	28.0	40.0
30	34.68544601560955	37.0	33.0	38.0	28.0	40.0
31	34.43646077651813	36.0	33.0	38.0	27.0	40.0
32	34.18888708633598	36.0	33.0	38.0	27.0	39.0
33	33.92872326677247	36.0	32.0	38.0	27.0	39.0
34	33.707697289763104	36.0	32.0	38.0	26.0	39.0
35	33.35462023612554	35.0	31.0	38.0	25.0	39.0
36	33.22498335832605	35.0	31.0	38.0	25.0	39.0
37	32.76139451603412	35.0	31.0	38.0	24.0	38.0
38	32.15783301033196	34.0	30.0	37.0	24.0	38.0
39	31.98655260256283	34.0	30.0	37.0	24.0	38.0
40	31.67121254123548	33.0	29.0	37.0	23.0	38.0
41	31.254865530525066	33.0	29.0	37.0	22.0	38.0
42	30.983427992357953	33.0	29.0	36.0	21.0	38.0
43	27.77583836416407	31.0	24.0	35.0	10.0	38.0
44	28.742245754842084	31.0	24.0	35.0	15.0	37.0
45	26.062873231564364	30.0	19.0	34.0	9.0	37.0
46	27.958125148887675	31.0	24.0	34.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	11.0
10	50.0
11	178.0
12	533.0
13	1331.0
14	3382.0
15	6750.0
16	11194.0
17	16143.0
18	22249.0
19	32176.0
20	50950.0
21	81026.0
22	126332.0
23	186683.0
24	262008.0
25	352068.0
26	451672.0
27	560864.0
28	686900.0
29	826851.0
30	997533.0
31	1250810.0
32	1718879.0
33	2744978.0
34	3898209.0
35	4958284.0
36	8630456.0
37	1.003542E7
38	2044277.0
39	46792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.27165786068188	38.629980909881496	15.484078361224437	18.61428286821219
2	29.214742960815638	29.75482058613188	26.333742365639885	14.696694087412595
3	24.597149005661546	25.24759536247853	26.516584556076626	23.638671075783297
4	21.991679038040004	30.71265859584017	24.095161628586833	23.20050073753299
5	22.465147472852763	30.92671939175588	23.21382407544659	23.39430905994477
6	24.138571213241146	25.687610470593793	28.46221433876124	21.71160397740382
7	21.506287090685436	26.937759514500563	27.624213879318553	23.931739515495444
8	23.476133852301924	25.61249984064488	27.4470509803902	23.464315326662998
9	24.76147850555643	26.182713706465115	26.64788817595005	22.407919612028397
10	20.512203602600575	36.048112997903516	24.87102733933942	18.568656060156496
11	21.879488033867776	29.53453806637622	27.011375330927468	21.574598568828538
12	21.942077725803706	26.179886559151743	29.24569659934923	22.632339115695316
13	22.18931188334255	26.883953726772585	26.354959718775085	24.57177467110978
14	22.15225900568904	29.15327812855346	26.470555298226543	22.22390756753095
15	22.316253547369964	28.517927388558277	26.23655948920372	22.929259574868034
16	23.016958634410358	26.361731374011093	25.83776673859936	24.78354325297919
17	25.032652176640962	26.203313636623832	26.039539067501327	22.724495119233875
18	22.529624429352438	26.35369737625231	25.9775792969827	25.13909889741255
19	23.23268172295506	28.72453411436923	25.645763191041915	22.39702097163379
20	25.100368729001055	26.596359604139387	25.959514050622186	22.343757616237376
21	23.098588451090727	28.368471033238603	25.919236575237235	22.61370394043343
22	25.19125239126419	26.220638975287834	26.17270245536869	22.415406178079284
23	22.665904928360188	26.45150767441762	28.551875653512226	22.330711743709973
24	22.659523224477745	28.26226428253075	25.951177590595577	23.127034902395927
25	22.79264161795816	26.399564154371742	25.81779922954611	24.989994998123986
26	22.708354632759566	27.685836191935053	27.25872197443369	22.34708720087169
27	22.99040944642156	25.760636360614015	28.537902396676014	22.711051796288412
28	24.75908880367174	26.178841689499233	26.58565843910972	22.4764110677193
29	24.623745687725457	26.061268856710125	26.880429166461482	22.434556289102932
30	24.476019116615202	25.841321295168427	26.881803994951632	22.80085559326474
31	23.983623043025382	26.488885511532434	26.682121405354682	22.8453700400875
32	23.347682376623517	26.701254018561183	27.089070638437857	22.861992966377446
33	23.278565998891636	26.271282657488477	27.122628952038237	23.327522391581653
34	23.376358799239792	26.58344121570834	26.96690087911533	23.073299105936535
35	23.16256297026946	26.503938633655448	27.214102540708048	23.119395855367042
36	23.26626003406075	26.23482720530614	27.15680218892693	23.34211057170618
37	23.489024744163164	26.532917518539563	26.841616508340582	23.136441228956688
38	23.4544615559209	26.448790513383457	27.16474119853548	22.93200673216016
39	23.34240053553319	26.186480736528118	27.177614592579573	23.293504135359115
40	23.453766642611335	26.552705050045006	26.857784491384702	23.13574381595896
41	23.402990476938	26.569702929559536	27.114359983592045	22.912946609910414
42	23.27276672235139	26.208278017317337	27.159839310046074	23.359115950285204
43	24.81805644745818	26.90665089530081	26.165913302315534	22.109379354925473
44	23.31251176415742	26.314149809811227	27.034045002885893	23.339293423145463
45	23.095543830907094	29.604044395461663	25.471812391404175	21.82859938222707
46	23.513656671330253	26.441453928622405	26.75541976138477	23.289469638662577
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5590.0
1	4739.5
2	3889.0
3	3542.0
4	3195.0
5	3630.5
6	4066.0
7	4066.0
8	5348.0
9	6630.0
10	9458.5
11	12287.0
12	19790.0
13	27293.0
14	39295.5
15	51298.0
16	73832.0
17	96366.0
18	135315.0
19	174264.0
20	174264.0
21	234060.0
22	293856.0
23	371559.5
24	449263.0
25	672600.0
26	895937.0
27	885311.5
28	874686.0
29	968352.5
30	1062019.0
31	1062019.0
32	1192713.0
33	1323407.0
34	1474299.0
35	1625191.0
36	1787592.5
37	1949994.0
38	2111653.5
39	2273313.0
40	2443905.5
41	2614498.0
42	2775249.5
43	2936001.0
44	2936001.0
45	3055093.5
46	3174186.0
47	3235197.5
48	3296209.0
49	3278082.5
50	3259956.0
51	3161155.0
52	3062354.0
53	2907699.5
54	2753045.0
55	2536541.0
56	2320037.0
57	2320037.0
58	2078131.5
59	1836226.0
60	1593055.5
61	1349885.0
62	1146081.0
63	942277.0
64	771902.5
65	601528.0
66	482433.5
67	363339.0
68	278669.0
69	193999.0
70	193999.0
71	143056.5
72	92114.0
73	67086.0
74	42058.0
75	30448.5
76	18839.0
77	13680.5
78	8522.0
79	6325.5
80	4129.0
81	4129.0
82	3088.0
83	2047.0
84	1452.0
85	857.0
86	576.5
87	296.0
88	163.0
89	30.0
90	20.5
91	11.0
92	6.5
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	4.000499E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.87995324589143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.14552398036804	26.777603570079588
2	16.10598652183161	12.846119789392041
3	6.150362850287349	7.358285487441811
4	2.990433369707254	4.770333718755153
5	1.7585749365493304	3.506594312449189
6	1.1115837807317368	2.659794552268373
7	0.7716987370011443	2.154271668606337
8	0.5722275274001183	1.8256325630983017
9	0.42831240028266593	1.5372970648117423
>10	2.5972076471773815	19.921965257420098
>50	0.2504573110110089	6.871528175215648
>100	0.11332677462966984	7.5726596010349425
>500	0.003337150367057251	0.8866227966844488
>1k	9.23274752567294E-4	0.5523935021633943
>5k	2.1868952033651717E-5	0.05983121555220242
>10k+	2.1868951100530637E-5	0.6990667250266624
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	196603	0.4914461920875371	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.49906449170466E-5	0.0	0.0	2.4996881639015532E-5	1.9997505311212427E-5
2	7.999002124484971E-5	0.0	0.0	2.7496569802917085E-5	1.9997505311212427E-5
3	8.998877390045592E-5	0.0	0.0	2.7496569802917085E-5	1.9997505311212427E-5
4	9.498815022825902E-5	0.0	0.0	2.999625796681864E-5	2.249719347511398E-5
5	9.998752655606213E-5	0.0	0.0	2.999625796681864E-5	2.7496569802917085E-5
6	1.074865910477668E-4	9.998752655606214E-6	0.0	2.999625796681864E-5	2.999625796681864E-5
7	1.17485343703373E-4	9.998752655606214E-6	0.0	3.74953224585233E-5	2.999625796681864E-5
8	1.1998503186727456E-4	9.998752655606214E-6	0.0	3.9995010622424854E-5	3.9995010622424854E-5
9	1.2498440819507767E-4	9.998752655606214E-6	0.0	3.9995010622424854E-5	4.249469878632641E-5
10	1.3748284901458544E-4	9.998752655606214E-6	0.0	4.249469878632641E-5	4.749407511412951E-5
11	1.4248222534238853E-4	9.998752655606214E-6	0.0	4.749407511412951E-5	4.9993763278031065E-5
12	1.5748035432579786E-4	9.998752655606214E-6	0.0	5.499313960583417E-5	5.749282776973572E-5
13	1.6747910698140407E-4	9.998752655606214E-6	0.0	5.499313960583417E-5	6.999126858924349E-5
14	1.824772359648134E-4	9.998752655606214E-6	0.0	5.749282776973572E-5	7.999002124484971E-5
15	2.074741176038289E-4	9.998752655606214E-6	0.0	5.749282776973572E-5	8.748908573655436E-5
16	2.299713110789429E-4	9.998752655606214E-6	0.0	6.499189226144038E-5	9.998752655606213E-5
17	2.524685045540569E-4	9.998752655606214E-6	0.0	6.749158042534194E-5	1.0248721471996369E-4
18	2.824647625208755E-4	9.998752655606214E-6	0.0	6.999126858924349E-5	1.0498690288386523E-4
19	3.224597731433004E-4	1.2498440819507766E-5	0.0	7.49906449170466E-5	1.499812898340932E-4
20	3.799526009130361E-4	1.499812898340932E-5	0.0	7.749033308094815E-5	1.5998004248969942E-4
21	4.449444931744765E-4	1.499812898340932E-5	0.0	8.248970940875125E-5	1.649794188175025E-4
22	5.099363854359169E-4	1.499812898340932E-5	0.0	8.248970940875125E-5	1.7997754780091184E-4
23	5.699289013695541E-4	1.499812898340932E-5	0.0	8.748908573655436E-5	1.824772359648134E-4
24	6.674167397617148E-4	1.499812898340932E-5	0.0	8.998877390045592E-5	1.824772359648134E-4
25	7.749033308094816E-4	1.499812898340932E-5	0.0	8.998877390045592E-5	1.874766122926165E-4
26	9.148858679879685E-4	1.499812898340932E-5	0.0	9.248846206435747E-5	1.924759886204196E-4
27	0.0010448696525108493	1.749781714731087E-5	0.0	9.748783839216058E-5	1.9997505311212426E-4
28	0.0012123487594922534	1.749781714731087E-5	0.0	1.0498690288386523E-4	2.0497442943992738E-4
29	0.0013398328558512326	1.749781714731087E-5	0.0	1.074865910477668E-4	2.074741176038289E-4
30	0.0015198104036521444	1.749781714731087E-5	0.0	1.0998627921166834E-4	2.149731820955336E-4
31	0.0017097867041086625	1.749781714731087E-5	0.0	1.17485343703373E-4	2.149731820955336E-4
32	0.0019372583270237038	1.749781714731087E-5	0.0	1.3998253717848697E-4	2.1747287025943515E-4
33	0.0022672171646587087	1.9997505311212427E-5	0.0	1.449819135062901E-4	2.299713110789429E-4
34	0.0024496944006235223	2.249719347511398E-5	0.0	2.149731820955336E-4	2.299713110789429E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	113070	0.0	34.957104	1
TCAACGC	133050	0.0	29.734686	12
CAACGCA	133645	0.0	29.602306	13
AACGCAG	135885	0.0	29.140818	14
ACGCAGA	135730	0.0	29.140207	15
ATCAACG	136415	0.0	29.049591	11
AGCAGTG	137360	0.0	28.78276	2
GCAGTGG	137565	0.0	28.716606	3
CGCAGAG	138580	0.0	28.553904	16
AGTGGTA	117005	0.0	28.38682	5
GTGGTAT	117325	0.0	28.307692	6
AGAGTAC	138530	0.0	28.210495	19
TGGTATC	118395	0.0	28.036657	7
GGTATCA	118780	0.0	27.981144	8
GTATCAA	119360	0.0	27.830091	9
CAGAGTA	143155	0.0	27.427614	18
TATCAAC	121415	0.0	27.4233	10
CAGTGGT	121565	0.0	27.285814	4
GCAGAGT	145830	0.0	27.087704	17
GAGTACT	48960	0.0	27.042482	20
>>END_MODULE