FastQCFastQC Report
Thu 26 May 2016
SRR805251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34400925
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT7784062.262747295312553No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC459700.13363012767825283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3956900.038.6570321
TCAACGC4074600.037.6184212
CAACGCA4081100.037.5452713
ATCAACG4092450.037.4802411
AGCAGTG4083450.037.4800722
GCAGTGG4092550.037.3160973
ACGCAGA4107750.037.29097415
AACGCAG4111200.037.29081314
GTGGTAT3507500.037.184896
CGCAGAG4131800.037.0797216
GTATCAA3525600.037.0121429
AGTGGTA3523350.037.0028535
GGTATCA3529200.036.9766548
TATCAAC3544250.036.92064710
TGGTATC3531200.036.9188967
AGAGTAC4099950.036.9121619
CAGAGTA4146100.036.6286418
GAGTACT1367550.036.59098420
GCAGAGT4174950.036.5494217
CAGTGGT3579950.036.3770454
Produced by FastQC (version 0.11.5)