##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805251_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34400925
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.39889473902228	37.0	37.0	37.0	35.0	37.0
2	36.330162895329124	37.0	37.0	37.0	35.0	37.0
3	38.15094451675355	39.0	39.0	39.0	37.0	39.0
4	38.07384644453601	39.0	39.0	39.0	37.0	39.0
5	38.045856528567185	39.0	39.0	39.0	35.0	39.0
6	37.92118909593274	39.0	38.0	39.0	35.0	39.0
7	37.85250492537628	39.0	38.0	39.0	35.0	39.0
8	38.818386773030085	40.0	39.0	40.0	36.0	40.0
9	38.68123403658477	40.0	39.0	40.0	36.0	40.0
10	33.40223962001022	40.0	36.0	40.0	3.0	40.0
11	35.259855483537144	40.0	36.0	40.0	17.0	40.0
12	37.35775962419615	40.0	36.0	40.0	32.0	40.0
13	38.16027005087799	40.0	37.0	40.0	34.0	40.0
14	38.28285707433739	40.0	38.0	40.0	35.0	40.0
15	38.34170589889661	40.0	38.0	40.0	35.0	40.0
16	38.327796854299706	40.0	38.0	40.0	35.0	40.0
17	38.043417059279655	40.0	38.0	40.0	34.0	40.0
18	38.03636381870545	40.0	38.0	40.0	34.0	40.0
19	37.93673998591608	40.0	38.0	40.0	34.0	40.0
20	37.64237665120923	39.0	37.0	40.0	34.0	40.0
21	37.28626442457579	38.0	36.0	40.0	33.0	40.0
22	37.36460917838692	39.0	37.0	40.0	33.0	40.0
23	36.97120589053928	38.0	36.0	40.0	33.0	40.0
24	36.85589000877157	38.0	36.0	40.0	33.0	40.0
25	36.53350146834715	38.0	36.0	40.0	32.0	40.0
26	36.47493138629267	38.0	36.0	39.0	31.0	40.0
27	35.815369644856936	38.0	35.0	39.0	30.0	40.0
28	35.47981044114366	38.0	34.0	39.0	30.0	40.0
29	35.048878831019806	37.0	34.0	39.0	29.0	40.0
30	34.9353513023269	37.0	34.0	38.0	29.0	40.0
31	34.729409717907295	37.0	33.0	38.0	29.0	40.0
32	34.45667292376586	36.0	33.0	38.0	28.0	40.0
33	34.231485490579104	36.0	33.0	38.0	27.0	39.0
34	34.003000064678496	36.0	32.0	38.0	27.0	39.0
35	33.70584817123377	35.0	32.0	38.0	27.0	39.0
36	33.587386996134555	35.0	32.0	38.0	27.0	39.0
37	32.96628520890063	35.0	31.0	38.0	25.0	38.0
38	32.31481569172922	34.0	30.0	37.0	24.0	38.0
39	32.19754239166534	34.0	30.0	37.0	24.0	38.0
40	31.89636825172579	34.0	30.0	37.0	24.0	38.0
41	31.495437520938754	33.0	29.0	36.0	23.0	38.0
42	31.182759562424557	33.0	29.0	36.0	23.0	38.0
43	27.679584197227253	31.0	23.0	35.0	10.0	37.0
44	28.89402872161141	31.0	24.0	35.0	15.0	37.0
45	26.058563541532678	30.0	19.0	34.0	9.0	37.0
46	28.009993219659066	31.0	24.0	34.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	12.0
12	65.0
13	314.0
14	1138.0
15	2663.0
16	4671.0
17	6554.0
18	8252.0
19	12556.0
20	23339.0
21	42779.0
22	73071.0
23	115710.0
24	170185.0
25	237469.0
26	312746.0
27	400544.0
28	498708.0
29	608748.0
30	744713.0
31	945211.0
32	1350753.0
33	2279191.0
34	3320297.0
35	4223384.0
36	7761798.0
37	9180030.0
38	2035234.0
39	40788.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.741782379398229	37.19161330690962	30.174749661527994	19.891854652164152
2	26.59424419546858	43.60753671594587	12.118020082308833	17.68019900627672
3	36.31639265513936	25.854592572728784	17.42277569571167	20.40623907642018
4	22.340663804825013	23.119055664927615	19.40587062702529	35.13440990322208
5	20.567813220138703	34.67422750987074	22.950499150822253	21.807460119168308
6	32.97510750074308	23.246962109303748	21.325289944965142	22.452640444988035
7	22.191327704124237	22.51153711709787	33.699669412959096	21.597465765818797
8	32.382707732422894	24.665019908621645	22.35445122478538	20.597821134170083
9	32.46661245300817	24.108284878967645	22.66201562894021	20.763087039083977
10	19.75445137013031	32.65969447042485	29.343504571461377	18.242349587983462
11	20.776534351910595	35.257008350792894	23.100623021037954	20.86583427625856
12	20.575095001079184	24.014525772199438	34.217181660086176	21.1931975666352
13	20.621079229700946	24.18058235352683	23.313800428331504	31.88453798844072
14	21.15085858883155	35.24731965782897	22.939301777495807	20.662519975843672
15	20.797783780523343	34.95465892268885	23.028744139874146	21.218813156913658
16	21.105467367519914	23.690644946320486	23.24243316131761	31.961454524841994
17	32.228717687097074	23.627873378404797	23.17848720637599	20.964921728122135
18	20.908844747633967	23.91807197044847	23.136331363182823	32.036751918734744
19	21.1798171124759	34.79137842950444	23.23152357095049	20.79728088706917
20	32.346290107024736	23.81538287124547	23.192216488364775	20.646110533365018
21	21.08903176295405	34.799631114570325	23.25719148540337	20.854145637072257
22	31.924830509644725	23.530274258613684	23.57831947832798	20.966575753413608
23	21.02803631007015	23.86375947739777	34.28957215539989	20.818632057132184
24	21.06588412956919	34.51503702298703	23.228450979152452	21.19062786829133
25	20.922559495129857	23.736980909670308	23.434893102438377	31.905566492761462
26	21.30565384506376	30.9123199448852	26.94396734971516	20.838058860335877
27	21.41175273629997	23.608743078856165	34.10097257559208	20.878531609251784
28	28.600925701852493	23.611792415465573	26.857042361506267	20.930239521175668
29	28.594495642195668	23.71208041644229	26.775070728476052	20.91835321288599
30	28.466720008255592	23.64536128025627	26.79106739135648	21.096851320131655
31	25.041131888168707	24.936471911729118	27.77771818635691	22.24467801374527
32	23.525146489520267	26.292115691656548	28.086192449766976	22.09654536905621
33	23.24231979227303	25.618069862947003	28.454900558633234	22.68470978614674
34	22.922444672635983	25.33316764011433	28.93619866326269	22.808189023986998
35	22.919308129069204	25.603919080664255	28.650941798803377	22.825830991463167
36	22.764472176256888	25.664426174586875	28.72212302430821	22.848978624848023
37	23.03730786308798	25.310755452069966	28.827774253163252	22.8241624316788
38	23.508210898398808	25.3865819015041	28.583638376002973	22.52156882409412
39	23.234433376428104	25.287081670042305	28.75552910277849	22.722955850751106
40	22.87869875592008	25.421086787637254	28.73248902464105	22.967725431801618
41	23.269708590684697	25.795460441834052	28.36919937472612	22.565631592755135
42	23.12877342687733	25.647946966542325	28.53798553381922	22.685294072761124
43	24.519599400306824	25.978298548658213	28.023659247534766	21.478442803500197
44	23.141316694245866	25.35917856859954	28.421404366307012	23.078100370847586
45	22.88852116621864	28.88512445522904	26.66352140240415	21.56283297614817
46	22.967129517592912	25.345085924288373	28.556589684725047	23.131194873393664
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3276.0
1	2398.0
2	1520.0
3	1275.5
4	1031.0
5	1157.5
6	1284.0
7	1284.0
8	1908.5
9	2533.0
10	4210.0
11	5887.0
12	9981.5
13	14076.0
14	20707.0
15	27338.0
16	40472.0
17	53606.0
18	80256.5
19	106907.0
20	106907.0
21	143607.5
22	180308.0
23	241342.0
24	302376.0
25	826381.5
26	1350387.0
27	1053774.0
28	757161.0
29	768447.0
30	779733.0
31	779733.0
32	855713.0
33	931693.0
34	1039722.5
35	1147752.0
36	1278701.5
37	1409651.0
38	1546666.5
39	1683682.0
40	1837961.5
41	1992241.0
42	2179704.5
43	2367168.0
44	2367168.0
45	2544157.5
46	2721147.0
47	2823771.0
48	2926395.0
49	2963102.5
50	2999810.0
51	2921529.0
52	2843248.0
53	2703447.0
54	2563646.0
55	2360293.5
56	2156941.0
57	2156941.0
58	1921216.5
59	1685492.0
60	1471054.5
61	1256617.0
62	1065286.5
63	873956.0
64	720578.5
65	567201.0
66	458295.5
67	349390.0
68	266485.0
69	183580.0
70	183580.0
71	134631.0
72	85682.0
73	61440.0
74	37198.0
75	27005.5
76	16813.0
77	12140.5
78	7468.0
79	5580.0
80	3692.0
81	3692.0
82	2726.0
83	1760.0
84	1281.5
85	803.0
86	616.0
87	429.0
88	230.0
89	31.0
90	20.0
91	9.0
92	7.5
93	6.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	3.4400925E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.56062377714901
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.20061480897978	29.877819558095435
2	15.084976502196245	12.840520191942154
3	5.330266728279126	6.805784305624285
4	2.5350393627722143	4.315714263168472
5	1.4422657950677906	3.069186594526547
6	0.9237727053543792	2.3589805540912114
7	0.6663903487536664	1.9853392245419543
8	0.48268597052956475	1.6434732795373472
9	0.3925225034389292	1.503540233363605
>10	2.7033537246378296	21.405266762972712
>50	0.1706554405625928	4.949583097314353
>100	0.06278215960420493	4.515788318921637
>500	0.0032522536856547375	0.9426299387365201
>1k	0.0013144454155834344	0.8729048545043756
>5k	5.720038557724944E-5	0.17590060464018445
>10k+	5.005033715358728E-5	2.7375682180192107
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	778406	2.262747295312553	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	45970	0.13363012767825283	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.592765310816497E-5	2.906898579035302E-6	0.0	1.453449289517651E-5	8.720695737105907E-6
2	1.308104360565886E-4	2.906898579035302E-6	0.0	1.7441391474211813E-5	8.720695737105907E-6
3	1.5697252326790631E-4	2.906898579035302E-6	1.453449289517651E-5	2.0348290053247116E-5	1.7441391474211813E-5
4	1.686001175840475E-4	2.906898579035302E-6	1.453449289517651E-5	2.6162087211317718E-5	2.0348290053247116E-5
5	1.8604150905825932E-4	5.813797158070604E-6	1.453449289517651E-5	3.197588436938832E-5	3.4882782948423626E-5
6	1.9766910337440055E-4	5.813797158070604E-6	1.453449289517651E-5	4.069658010649423E-5	4.069658010649423E-5
7	2.0638979911150645E-4	5.813797158070604E-6	1.453449289517651E-5	4.360347868552953E-5	5.2324174422635436E-5
8	2.0929669769054174E-4	8.720695737105907E-6	1.453449289517651E-5	4.9417275843600136E-5	5.2324174422635436E-5
9	2.2092429200668297E-4	1.7441391474211813E-5	1.453449289517651E-5	6.395176873877664E-5	5.813797158070604E-5
10	2.2964498774378887E-4	1.7441391474211813E-5	1.453449289517651E-5	7.267246447588256E-5	6.685866731781195E-5
11	2.441794806389654E-4	1.7441391474211813E-5	1.453449289517651E-5	8.139316021298846E-5	9.883455168720027E-5
12	2.470863792180007E-4	1.7441391474211813E-5	1.453449289517651E-5	8.720695737105906E-5	1.1627594316141208E-4
13	2.645277706922125E-4	2.0348290053247116E-5	1.453449289517651E-5	9.011385595009437E-5	1.220897403194827E-4
14	2.703415678502831E-4	2.0348290053247116E-5	1.453449289517651E-5	9.883455168720027E-5	1.366242332146592E-4
15	2.9068985790353023E-4	2.0348290053247116E-5	1.453449289517651E-5	1.1627594316141208E-4	1.395311317936945E-4
16	3.2557264085195385E-4	2.0348290053247116E-5	1.453449289517651E-5	1.24996638898518E-4	1.5406562468887102E-4
17	3.575485252213422E-4	3.197588436938832E-5	1.453449289517651E-5	1.308104360565886E-4	1.6278632042597693E-4
18	4.069658010649423E-4	3.4882782948423626E-5	1.453449289517651E-5	1.4534492895176512E-4	1.686001175840475E-4
19	4.4184858401336594E-4	3.4882782948423626E-5	1.453449289517651E-5	1.4534492895176512E-4	1.8313461047922403E-4
20	4.970796570150367E-4	3.4882782948423626E-5	1.453449289517651E-5	1.4534492895176512E-4	2.1801739342764765E-4
21	5.610314257538133E-4	3.4882782948423626E-5	1.453449289517651E-5	1.5406562468887102E-4	2.2673808916475355E-4
22	6.511452817039077E-4	4.069658010649423E-5	1.453449289517651E-5	1.5697252326790631E-4	2.3836568348089478E-4
23	7.383522390749667E-4	4.360347868552953E-5	1.453449289517651E-5	1.7150701616308283E-4	2.441794806389654E-4
24	8.139316021298846E-4	4.360347868552953E-5	1.453449289517651E-5	1.7732081332115342E-4	2.49993277797036E-4
25	9.214868495541908E-4	4.9417275843600136E-5	1.453449289517651E-5	1.8604150905825932E-4	2.5290017637607127E-4
26	0.0010232282998204262	4.9417275843600136E-5	1.453449289517651E-5	1.9185530621632993E-4	2.5580707495510656E-4
27	0.0011395042429818384	4.9417275843600136E-5	1.453449289517651E-5	1.9766910337440055E-4	2.703415678502831E-4
28	0.0013022905634078154	4.9417275843600136E-5	1.453449289517651E-5	2.0348290053247116E-4	2.848760607454596E-4
29	0.0014708906809918628	5.2324174422635436E-5	1.453449289517651E-5	2.2673808916475355E-4	2.877829593244949E-4
30	0.0016336770014178398	5.523107300167074E-5	1.453449289517651E-5	2.2673808916475355E-4	2.9068985790353023E-4
31	0.0018487874962664522	5.523107300167074E-5	1.7441391474211813E-5	2.2673808916475355E-4	2.9068985790353023E-4
32	0.0020522703967989233	5.813797158070604E-5	1.7441391474211813E-5	2.674346692712478E-4	2.965036550616008E-4
33	0.002261567094489465	5.813797158070604E-5	1.7441391474211813E-5	2.819691621664243E-4	2.994105536406361E-4
34	0.0024766775893380775	5.813797158070604E-5	2.0348290053247116E-5	3.197588436938832E-4	3.052243507987067E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	395690	0.0	38.657032	1
TCAACGC	407460	0.0	37.61842	12
CAACGCA	408110	0.0	37.54527	13
ATCAACG	409245	0.0	37.48024	11
AGCAGTG	408345	0.0	37.480072	2
GCAGTGG	409255	0.0	37.316097	3
ACGCAGA	410775	0.0	37.290974	15
AACGCAG	411120	0.0	37.290813	14
GTGGTAT	350750	0.0	37.18489	6
CGCAGAG	413180	0.0	37.07972	16
GTATCAA	352560	0.0	37.012142	9
AGTGGTA	352335	0.0	37.002853	5
GGTATCA	352920	0.0	36.976654	8
TATCAAC	354425	0.0	36.920647	10
TGGTATC	353120	0.0	36.918896	7
AGAGTAC	409995	0.0	36.91216	19
CAGAGTA	414610	0.0	36.62864	18
GAGTACT	136755	0.0	36.590984	20
GCAGAGT	417495	0.0	36.54942	17
CAGTGGT	357995	0.0	36.377045	4
>>END_MODULE