FastQCFastQC Report
Thu 26 May 2016
SRR805250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32447701
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT3601711.1100046810712414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1703700.037.1661641
TCAACGC1782200.035.45393412
CAACGCA1788200.035.34168613
ATCAACG1797900.035.1832711
AACGCAG1807200.035.00332314
ACGCAGA1810500.034.91190315
CGCAGAG1834900.034.46182316
GTATCAA1550800.034.3796739
GCAGTGG1839200.034.316013
AGCAGTG1843250.034.305712
GTGGTAT1553500.034.3006136
GGTATCA1554200.034.294178
AGAGTAC1828200.034.18663419
AGTGGTA1564750.034.0731745
TGGTATC1565150.034.0210237
TATCAAC1574300.033.94524410
CAGAGTA1864700.033.64401618
CAGTGGT1589750.033.504644
GCAGAGT1890000.033.35872717
GAGTACT680700.033.0806520
Produced by FastQC (version 0.11.5)