##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805250_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32447701
Sequences flagged as poor quality	0
Sequence length	46
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.538867514835644	35.0	35.0	35.0	33.0	36.0
2	35.39155294854326	35.0	35.0	37.0	35.0	37.0
3	38.020412447710854	39.0	39.0	39.0	35.0	39.0
4	38.043631904768844	39.0	39.0	39.0	35.0	39.0
5	38.04606899576645	39.0	39.0	39.0	35.0	39.0
6	37.947580292360314	39.0	38.0	39.0	35.0	39.0
7	37.92583613242738	39.0	38.0	39.0	35.0	39.0
8	38.84238994312725	40.0	39.0	40.0	36.0	40.0
9	38.711361584600404	40.0	39.0	40.0	36.0	40.0
10	33.206785251133816	40.0	36.0	40.0	3.0	40.0
11	35.17114966018702	40.0	36.0	40.0	17.0	40.0
12	37.31835685369512	40.0	36.0	40.0	32.0	40.0
13	38.119138702615636	40.0	37.0	40.0	34.0	40.0
14	38.26322145288506	40.0	38.0	40.0	35.0	40.0
15	38.31391034452641	40.0	38.0	40.0	35.0	40.0
16	38.29405001605507	40.0	38.0	40.0	35.0	40.0
17	38.076831329282776	40.0	38.0	40.0	34.0	40.0
18	38.00036871641538	40.0	38.0	40.0	34.0	40.0
19	37.89612592275798	39.0	38.0	40.0	34.0	40.0
20	37.63037057694781	39.0	37.0	40.0	34.0	40.0
21	37.396820317100435	39.0	37.0	40.0	33.0	40.0
22	37.31397968688136	39.0	37.0	40.0	33.0	40.0
23	37.09864091757996	38.0	36.0	40.0	33.0	40.0
24	36.87672987987654	38.0	36.0	40.0	33.0	40.0
25	36.59089382018159	38.0	36.0	40.0	32.0	40.0
26	36.45923755892598	38.0	36.0	40.0	31.0	40.0
27	35.83900366315629	38.0	35.0	39.0	30.0	40.0
28	35.53793022192851	38.0	34.0	39.0	30.0	40.0
29	35.20821034439389	37.0	34.0	39.0	29.0	40.0
30	35.06584549703537	37.0	34.0	38.0	29.0	40.0
31	34.85542541211163	37.0	33.0	38.0	29.0	40.0
32	34.60836855591094	36.0	33.0	38.0	29.0	40.0
33	34.358898770671	36.0	33.0	38.0	28.0	39.0
34	34.1178074218571	36.0	32.0	38.0	27.0	39.0
35	33.80170302358247	35.0	32.0	38.0	27.0	39.0
36	33.68907877325423	35.0	32.0	38.0	27.0	39.0
37	33.099469604949824	35.0	31.0	38.0	26.0	38.0
38	32.52621275078934	34.0	31.0	37.0	24.0	38.0
39	32.37435336327834	34.0	30.0	37.0	24.0	38.0
40	32.077864592009156	34.0	30.0	37.0	24.0	38.0
41	31.668343005256368	33.0	29.0	37.0	24.0	38.0
42	31.37699339623476	33.0	29.0	36.0	23.0	38.0
43	28.011193242935764	31.0	24.0	35.0	10.0	38.0
44	29.170344703311955	31.0	25.0	35.0	15.0	37.0
45	26.43610461030814	30.0	21.0	34.0	9.0	37.0
46	28.383411293145237	31.0	24.0	34.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	16.0
12	69.0
13	399.0
14	1490.0
15	3274.0
16	5349.0
17	6825.0
18	8041.0
19	11736.0
20	20553.0
21	36707.0
22	63022.0
23	99262.0
24	146024.0
25	204653.0
26	271450.0
27	349300.0
28	439236.0
29	542824.0
30	678174.0
31	883649.0
32	1295689.0
33	2197620.0
34	3199781.0
35	4233721.0
36	7665802.0
37	8421351.0
38	1631228.0
39	30455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.275587721915954	44.69729920156747	21.81591231995142	21.21120075656516
2	28.109704906366094	40.56565671632637	17.986534700871413	13.338103676436122
3	31.28456157802983	25.885402481981696	22.593480505752936	20.23655543423554
4	21.62166126962277	27.223025754582736	22.474655446313438	28.680657529481056
5	20.24256510499773	32.27941480353261	26.11075897179896	21.367261119670697
6	26.542937510426395	24.84589277989217	25.1951101250594	23.416059584622033
7	23.338013993657057	25.423989206508036	29.413190167155452	21.82480663267946
8	25.537007999426525	26.041459763204795	26.4193817614382	22.00215047593048
9	26.667975644869262	25.086208727083626	26.442985899062617	21.802829728984495
10	20.416444912383778	35.32069960827117	25.594266293319208	18.668589186025844
11	21.609641928098387	30.992457678280505	25.71334406711896	21.684556326502147
12	21.74228306652604	25.696498497690172	30.213746114092956	22.347472321690834
13	21.790277221797623	26.542040682635726	25.221879355951906	26.44580273961474
14	22.041493787186955	31.18760863828226	25.150213261642172	21.620684312888606
15	21.94323104740148	30.642026071431072	25.076226509853498	22.33851637131395
16	22.340612051374613	25.896179824881894	25.14315575084965	26.620052372893845
17	26.974320923383754	25.715840391897103	25.254417870776113	22.055420813943027
18	22.15285452735157	25.612640476439303	25.163212641783154	27.071292354425974
19	22.36583109539872	30.67072147884992	25.060240785626075	21.90320664012529
20	27.157252219502393	26.054292721693905	25.122137312594194	21.666317746209508
21	22.612985739729297	30.27472115821087	25.03269800224059	22.079595099819244
22	26.770066082647894	25.824513730572157	25.410663146828185	21.994757039951768
23	22.13505665624816	25.915663485681158	30.200913155603843	21.74836670246684
24	22.37277765842332	30.028623599557946	25.124371677364753	22.474227064653977
25	22.06345528146971	25.91240593593981	25.132714333135652	26.89142444945483
26	22.218514032781552	28.62681704321671	27.280188510119714	21.87448041388202
27	22.5992251346251	25.26760216386363	29.9741174266861	22.15905527482517
28	25.449023337585615	25.670958937892085	26.815184841600953	22.06483288292135
29	25.468124844962052	25.629322706098655	26.948824509939858	21.95372793899944
30	25.557447043782854	25.21372161312754	26.895045661324357	22.33378568176525
31	24.13846823847397	26.12925951209918	27.094270869914638	22.638001379512218
32	23.43542305200606	26.698575655637356	27.454031334916458	22.411969957440128
33	23.40436384075408	26.09202112655069	27.444526809464868	23.05908822323036
34	23.039958978911944	26.434159387748302	27.54990869769171	22.975972935648045
35	23.022262809929124	26.381440706692906	27.702970389181043	22.893326094196937
36	23.167752316258092	26.05821287616032	27.65267406772517	23.12136073985642
37	23.192521405445643	26.309703729087	27.464294003448813	23.033480862018546
38	23.39300402207232	26.289150655080306	27.74962392559029	22.568221397257084
39	23.30252919921815	25.93912893859568	27.734199103967335	23.024142758218833
40	23.088387679607873	26.41082645577879	27.55174858150967	22.949037283103664
41	23.356101561710027	26.443469754606035	27.555582443267706	22.644846240416232
42	23.307752989957596	26.071927253027884	27.533611703337623	23.0867080536769
43	24.493430212513363	26.832350926803723	26.922394286115985	21.751824574566932
44	23.170242477271348	26.2633830359815	27.587529236663023	22.978845250084127
45	23.28676537052656	29.06228086852748	25.94468249075643	21.70627127018953
46	23.08966666082136	26.29726525155049	27.443941251800858	23.16912683582729
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2343.0
1	1951.5
2	1560.0
3	1538.0
4	1516.0
5	1760.5
6	2005.0
7	2005.0
8	2879.5
9	3754.0
10	5997.0
11	8240.0
12	14036.5
13	19833.0
14	28207.0
15	36581.0
16	52283.0
17	67985.0
18	99932.0
19	131879.0
20	131879.0
21	178339.5
22	224800.0
23	286316.5
24	347833.0
25	637885.5
26	927938.0
27	826055.0
28	724172.0
29	787200.0
30	850228.0
31	850228.0
32	957305.5
33	1064383.0
34	1184314.5
35	1304246.0
36	1438342.0
37	1572438.0
38	1703383.5
39	1834329.0
40	1972119.5
41	2109910.0
42	2249868.0
43	2389826.0
44	2389826.0
45	2501866.5
46	2613907.0
47	2667112.0
48	2720317.0
49	2704782.5
50	2689248.0
51	2603882.0
52	2518516.0
53	2377372.5
54	2236229.0
55	2053604.0
56	1870979.0
57	1870979.0
58	1656543.0
59	1442107.0
60	1250556.5
61	1059006.0
62	891070.5
63	723135.0
64	587259.5
65	451384.0
66	356256.0
67	261128.0
68	197454.0
69	133780.0
70	133780.0
71	96269.0
72	58758.0
73	41939.5
74	25121.0
75	18018.0
76	10915.0
77	7636.5
78	4358.0
79	3046.0
80	1734.0
81	1734.0
82	1287.0
83	840.0
84	571.5
85	303.0
86	209.0
87	115.0
88	64.5
89	14.0
90	9.5
91	5.0
92	4.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	3.2447701E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.45417734441735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.00039901775054	25.645372703042213
2	16.9130811327083	13.345834049007847
3	6.754092539616202	7.994314945758715
4	3.2150097555481225	5.0738226023771
5	1.8591949368931393	3.667650337901237
6	1.1994461284693883	2.839389616062362
7	0.8312908001803103	2.29585262585676
8	0.6228039356666195	1.965777354287352
9	0.4827353754200739	1.7141334401022736
>10	2.792272892919456	20.454346416696666
>50	0.22816663800120873	6.203858535529833
>100	0.09834363069984486	6.316744668314636
>500	0.0025065973230213416	0.6511777519244865
>1k	5.743243664839427E-4	0.345406918546237
>5k	4.1147218768578845E-5	0.10585410782361486
>10k+	4.114721850016843E-5	1.3804639267685714
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	360171	1.1100046810712414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.163764884297966E-5	3.081882442148983E-6	0.0	1.5409412210744916E-5	6.163764884297966E-6
2	6.471953128512864E-5	3.081882442148983E-6	0.0	1.5409412210744916E-5	9.245647326446949E-6
3	7.396517861157559E-5	3.081882442148983E-6	0.0	1.5409412210744916E-5	9.245647326446949E-6
4	1.0170212059091644E-4	3.081882442148983E-6	0.0	1.8491294652893898E-5	1.5409412210744916E-5
5	1.0478400303306542E-4	9.245647326446949E-6	0.0	1.8491294652893898E-5	1.8491294652893898E-5
6	1.1094776791736339E-4	9.245647326446949E-6	0.0	1.8491294652893898E-5	1.8491294652893898E-5
7	1.2019341524381034E-4	9.245647326446949E-6	0.0	1.8491294652893898E-5	1.8491294652893898E-5
8	1.2019341524381034E-4	9.245647326446949E-6	0.0	1.8491294652893898E-5	2.1573177095042883E-5
9	1.2327529768595933E-4	1.2327529768595932E-5	0.0	2.4655059537191865E-5	2.7736941979340846E-5
10	1.2327529768595933E-4	1.2327529768595932E-5	0.0	3.390070686363881E-5	4.006447174793678E-5
11	1.294390625702573E-4	1.2327529768595932E-5	0.0	4.3146354190085765E-5	6.471953128512864E-5
12	1.4176659233885322E-4	1.2327529768595932E-5	0.0	4.3146354190085765E-5	7.704706105372457E-5
13	1.448484747810022E-4	1.2327529768595932E-5	0.0	4.3146354190085765E-5	8.937459082232051E-5
14	1.602578869917471E-4	1.2327529768595932E-5	0.0	4.3146354190085765E-5	1.0170212059091644E-4
15	1.664216518760451E-4	1.2327529768595932E-5	0.0	4.622823663223475E-5	1.0170212059091644E-4
16	1.7258541676034306E-4	1.2327529768595932E-5	0.0	4.622823663223475E-5	1.1402965035951237E-4
17	2.0340424118183288E-4	1.2327529768595932E-5	0.0	4.622823663223475E-5	1.2019341524381034E-4
18	2.2189553583472677E-4	1.2327529768595932E-5	0.0	4.622823663223475E-5	1.2327529768595933E-4
19	2.6504189002481257E-4	1.2327529768595932E-5	0.0	4.622823663223475E-5	1.263571801281083E-4
20	3.112701266570473E-4	1.2327529768595932E-5	0.0	4.931011907438373E-5	1.3252094501240629E-4
21	3.60580245731431E-4	1.2327529768595932E-5	0.0	4.931011907438373E-5	1.4793035722315118E-4
22	3.852353052686229E-4	1.2327529768595932E-5	0.0	4.931011907438373E-5	1.4793035722315118E-4
23	4.2221789457441066E-4	1.2327529768595932E-5	0.0	5.239200151653271E-5	1.7566729920249203E-4
24	4.900193083016883E-4	1.2327529768595932E-5	0.0	5.855576640083068E-5	1.7874918164464103E-4
25	5.63984486913264E-4	1.2327529768595932E-5	0.0	6.163764884297966E-5	1.7874918164464103E-4
26	6.471953128512865E-4	1.5409412210744916E-5	0.0	6.780141372727763E-5	1.84912946528939E-4
27	7.21160491462862E-4	1.5409412210744916E-5	0.0	6.780141372727763E-5	1.9724047629753492E-4
28	8.166988471694805E-4	1.5409412210744916E-5	0.0	7.704706105372457E-5	2.1264988850827984E-4
29	9.368922624132909E-4	1.5409412210744916E-5	0.0	8.012894349587355E-5	2.157317709504288E-4
30	0.0010293487356777603	1.5409412210744916E-5	0.0	8.012894349587355E-5	2.2497741827687577E-4
31	0.0011711153280166136	1.5409412210744916E-5	0.0	8.321082593802255E-5	2.2497741827687577E-4
32	0.0013128819203554669	1.8491294652893898E-5	0.0	1.078658854752144E-4	2.3422306560332272E-4
33	0.0016642165187604509	2.1573177095042883E-5	0.0	1.078658854752144E-4	2.4346871292976966E-4
34	0.0018398838179629429	2.4655059537191865E-5	0.0	1.448484747810022E-4	2.4346871292976966E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	170370	0.0	37.166164	1
TCAACGC	178220	0.0	35.453934	12
CAACGCA	178820	0.0	35.341686	13
ATCAACG	179790	0.0	35.18327	11
AACGCAG	180720	0.0	35.003323	14
ACGCAGA	181050	0.0	34.911903	15
CGCAGAG	183490	0.0	34.461823	16
GTATCAA	155080	0.0	34.379673	9
GCAGTGG	183920	0.0	34.31601	3
AGCAGTG	184325	0.0	34.30571	2
GTGGTAT	155350	0.0	34.300613	6
GGTATCA	155420	0.0	34.29417	8
AGAGTAC	182820	0.0	34.186634	19
AGTGGTA	156475	0.0	34.073174	5
TGGTATC	156515	0.0	34.021023	7
TATCAAC	157430	0.0	33.945244	10
CAGAGTA	186470	0.0	33.644016	18
CAGTGGT	158975	0.0	33.50464	4
GCAGAGT	189000	0.0	33.358727	17
GAGTACT	68070	0.0	33.08065	20
>>END_MODULE