FastQCFastQC Report
Thu 26 May 2016
SRR805249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19431497
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT145850.023.7641411
AGCAGTG231900.015.1013372
CAACGCA235650.014.51304913
TCAACGC237650.014.35724712
ACGCAGA245250.014.12436315
AACGCAG242500.014.09484514
AGTGGTA246250.014.0182755
GCAGTGG255800.013.7607513
ATCAACG249500.013.74749511
GGTATCA249600.013.637828
GTATCAA251550.013.5718559
CGCAGAG263800.013.22971816
TGGTATC262400.013.1707327
GTGGTAT261100.013.1673696
AGAGTAC259600.013.13559219
TATCAAC262250.013.03336510
CAGTGGT272650.012.7636174
CAGAGTA284050.012.15983218
GAGTACT86950.012.07590620
GCAGAGT295550.011.77465817
Produced by FastQC (version 0.11.5)