##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805249_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19431497
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.10216515999771	37.0	35.0	37.0	35.0	37.0
2	36.033229966790515	37.0	35.0	37.0	35.0	37.0
3	37.85850071149948	39.0	38.0	39.0	35.0	39.0
4	37.87920956373047	39.0	38.0	39.0	35.0	39.0
5	37.85683527110649	39.0	38.0	39.0	35.0	39.0
6	37.823020635003054	39.0	38.0	39.0	35.0	39.0
7	37.82537861081933	39.0	38.0	39.0	35.0	39.0
8	38.77952434647727	40.0	39.0	40.0	36.0	40.0
9	38.796866808563436	40.0	39.0	40.0	36.0	40.0
10	38.6300818717158	40.0	39.0	40.0	36.0	40.0
11	38.701429436960005	40.0	39.0	40.0	36.0	40.0
12	38.73420699393361	40.0	39.0	40.0	36.0	40.0
13	38.81593914251692	40.0	39.0	40.0	36.0	40.0
14	38.57731378081679	40.0	39.0	40.0	36.0	40.0
15	38.67274029376121	40.0	39.0	40.0	36.0	40.0
16	38.7228063797658	40.0	39.0	40.0	36.0	40.0
17	38.702487667316625	40.0	39.0	40.0	36.0	40.0
18	38.649488662659394	40.0	39.0	40.0	36.0	40.0
19	38.60846202431033	40.0	39.0	40.0	36.0	40.0
20	38.49787888189984	40.0	39.0	40.0	35.0	40.0
21	38.49548678622136	40.0	39.0	40.0	35.0	40.0
22	38.41439848921573	40.0	39.0	40.0	35.0	40.0
23	38.431068846625664	40.0	39.0	40.0	35.0	40.0
24	38.351969742732635	40.0	39.0	40.0	35.0	40.0
25	38.32755716144773	40.0	38.0	40.0	35.0	40.0
26	38.30641509503874	40.0	38.0	40.0	35.0	40.0
27	38.070559720643246	40.0	38.0	40.0	34.0	40.0
28	37.975699196001216	40.0	38.0	40.0	34.0	40.0
29	37.85425461558623	40.0	38.0	40.0	34.0	40.0
30	37.81967678558168	40.0	38.0	40.0	33.0	40.0
31	37.629699605748336	40.0	38.0	40.0	33.0	40.0
32	37.636600154892854	40.0	38.0	40.0	33.0	40.0
33	37.720003713558455	40.0	38.0	40.0	33.0	40.0
34	37.726593066916045	40.0	38.0	40.0	33.0	40.0
35	37.677290637978125	40.0	38.0	40.0	33.0	40.0
36	37.80841568717017	40.0	38.0	40.0	33.0	40.0
37	37.789226378183834	40.0	38.0	40.0	33.0	40.0
38	37.69354440370703	40.0	38.0	40.0	33.0	40.0
39	37.61687666163858	40.0	38.0	40.0	33.0	40.0
40	37.11228625360156	40.0	37.0	40.0	31.0	40.0
41	37.1103426051014	40.0	37.0	40.0	31.0	40.0
42	37.25235713954514	40.0	37.0	40.0	32.0	40.0
43	34.9338491522295	39.0	34.0	40.0	22.0	40.0
44	36.00960013528551	39.0	35.0	40.0	27.0	40.0
45	31.363662768751166	38.0	26.0	40.0	9.0	40.0
46	33.31622339750766	38.0	30.0	40.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	8.0
13	46.0
14	131.0
15	390.0
16	1002.0
17	1867.0
18	3010.0
19	4596.0
20	7449.0
21	12353.0
22	19588.0
23	31001.0
24	45041.0
25	64520.0
26	86905.0
27	113698.0
28	145694.0
29	182737.0
30	225376.0
31	276899.0
32	339845.0
33	423680.0
34	549273.0
35	761090.0
36	1188002.0
37	2176775.0
38	3997902.0
39	8772617.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.411644506854003	28.973089412514124	22.710139110743757	24.905126969888116
2	25.421803580032975	23.647375186790807	28.990555900042082	21.94026533313414
3	20.889435332748683	25.87210342054449	27.439193182079592	25.79926806462724
4	22.033994601651123	28.844473485496252	26.52891334105653	22.592618571796088
5	25.535515868900887	27.662984483387977	25.16854465716151	21.63295499054962
6	24.201897568674198	26.186273759556457	27.286693351520984	22.325135320248357
7	23.865371772437296	27.098442286767714	26.81541211158358	22.22077382921141
8	22.944397953487577	26.1502703574511	27.47923641703982	23.426095272021502
9	22.65325208860645	26.13242304491517	28.170320588269654	23.044004278208725
10	22.998819905640826	26.81260738686268	27.420573926959925	22.76799878053657
11	22.645738514124776	27.096949864439164	27.265897218315192	22.99141440312087
12	22.666148676038702	26.604002769318285	27.34204678105861	23.387801773584403
13	22.918182783343973	26.981374620802505	26.600333468903603	23.50010912694992
14	23.472216268257664	27.05462167943108	26.521003502715207	22.95215854959605
15	23.087006626406602	26.780443112540432	26.611789096846216	23.520761164206753
16	23.385038219134636	26.700912441280256	26.408634393942986	23.50541494564212
17	23.85974688414382	26.420553187435843	26.521111574676926	23.19858835374341
18	23.24361319151067	26.561175394772725	26.504700075346744	23.690511338369863
19	23.650468103409633	26.93531537997304	26.31433388791404	23.099882628703288
20	23.79696736695068	26.487120369573177	26.617532349669197	23.098379913806948
21	23.463488170777577	26.736993037643984	26.561653999174638	23.237864792403798
22	23.68750590857719	26.45656688210898	26.604188035538385	23.251739173775444
23	23.32330339757148	26.564268311391555	26.974545502078406	23.137882788958567
24	23.27814475642304	26.685535345012273	26.557367144692968	23.478952753871717
25	23.43954765811404	26.494824356558837	26.49493757480445	23.57069041052267
26	23.59748711074602	26.475649302778887	26.90693877059498	23.019924815880113
27	23.630613740155994	26.136524633176744	26.982954529957215	23.24990709671005
28	23.97174031419195	26.355015262076826	26.52034992466098	23.152894499070246
29	23.89836974474998	26.271671194453006	26.708889181312173	23.12106987948484
30	23.72717346481334	26.229970856079692	26.64525538099303	23.397600298113932
31	24.09476737690359	26.43116997110413	26.328599386861445	23.145463265130832
32	23.646567220219833	26.446083901821872	26.624809195091864	23.282539682866428
33	23.53770787706166	26.282601901438678	26.661718343162132	23.517971878337526
34	23.649845403058755	26.490022873688012	26.480718392412072	23.37941333084116
35	23.630567423600972	26.36677966705293	26.624973876176394	23.377679033169706
36	23.60015288580185	26.304103075537615	26.57424695585729	23.521497082803243
37	23.69425268675903	26.48708434558593	26.390838544245977	23.427824423409067
38	23.648244908768483	26.459489971359385	26.572584706160313	23.319680413711822
39	23.58897001090549	26.319665438025698	26.572075224055048	23.519289327013766
40	23.994713325483875	26.31977350998742	26.2899662336875	23.395546930841203
41	23.869035926568085	26.330426317642946	26.51926920504375	23.281268550745217
42	23.56710859693414	26.290259571869324	26.618541021311948	23.52409080988459
43	25.38525467183511	26.840474514135476	25.530523973526076	22.243746840503334
44	23.695395161782955	26.314781614612603	26.569167573656316	23.42065564994812
45	24.25961828880194	30.126062855579267	24.420537439807134	21.193781415811657
46	23.74977594366507	26.409617334166274	26.373521298950877	23.467085423217778
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2123.0
1	1703.0
2	1283.0
3	1254.5
4	1226.0
5	1425.5
6	1625.0
7	1625.0
8	2156.5
9	2688.0
10	3865.5
11	5043.0
12	7973.5
13	10904.0
14	15261.0
15	19618.0
16	28546.0
17	37474.0
18	54733.0
19	71992.0
20	71992.0
21	98404.5
22	124817.0
23	160884.5
24	196952.0
25	254182.0
26	311412.0
27	341873.0
28	372334.0
29	426137.0
30	479940.0
31	479940.0
32	552895.0
33	625850.0
34	707107.5
35	788365.0
36	867283.0
37	946201.0
38	1026266.5
39	1106332.0
40	1187811.0
41	1269290.0
42	1342973.5
43	1416657.0
44	1416657.0
45	1464410.5
46	1512164.0
47	1540646.0
48	1569128.0
49	1565098.5
50	1561069.0
51	1524262.5
52	1487456.0
53	1421062.5
54	1354669.0
55	1262503.0
56	1170337.0
57	1170337.0
58	1060767.0
59	951197.0
60	837637.0
61	724077.0
62	624126.5
63	524176.0
64	436809.5
65	349443.0
66	283090.5
67	216738.0
68	167504.0
69	118270.0
70	118270.0
71	87117.0
72	55964.0
73	40320.0
74	24676.0
75	18083.0
76	11490.0
77	8135.0
78	4780.0
79	3421.5
80	2063.0
81	2063.0
82	1502.5
83	942.0
84	683.5
85	425.0
86	323.0
87	221.0
88	135.5
89	50.0
90	34.5
91	19.0
92	16.5
93	14.0
94	14.0
95	8.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.9431497E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.29849980093576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.41322205523005	34.25036768964416
2	14.494191495640132	13.711070271425191
3	4.995703423636041	7.088678321651501
4	2.2786142778297815	4.311001478653651
5	1.340519256011256	3.170227488179947
6	0.802198569666822	2.2765673332618275
7	0.5837382696843104	1.9326961102723361
8	0.4029453294553151	1.5246967668024167
9	0.31791943152843016	1.3533400951974954
>10	2.1257740688246893	18.893036595044855
>50	0.17034903073224217	5.631126881358462
>100	0.07385886986668734	5.374226203554532
>500	8.556153975881604E-4	0.25886988584498977
>1k	7.725670251113941E-5	0.05525303426488955
>5k	2.2033195986269772E-5	0.07727967783479554
>10k+	1.1016597993134208E-5	0.09156216700916771
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.007050717708471E-4	5.146283891560182E-6	0.0	0.0	1.0292567783120364E-5
2	2.007050717708471E-4	5.146283891560182E-6	0.0	0.0	1.0292567783120364E-5
3	2.007050717708471E-4	5.146283891560182E-6	0.0	0.0	1.5438851674680545E-5
4	2.0585135566240727E-4	5.146283891560182E-6	0.0	0.0	3.602398724092127E-5
5	2.0585135566240727E-4	1.0292567783120364E-5	0.0	0.0	4.6316555024041634E-5
6	2.1099763955396747E-4	1.5438851674680545E-5	0.0	0.0	6.175540669872218E-5
7	2.212902073370878E-4	1.5438851674680545E-5	0.0	0.0	6.175540669872218E-5
8	2.212902073370878E-4	1.5438851674680545E-5	0.0	0.0	8.234054226496291E-5
9	2.212902073370878E-4	1.5438851674680545E-5	0.0	0.0	8.74868261565231E-5
10	2.212902073370878E-4	1.5438851674680545E-5	0.0	0.0	9.263311004808327E-5
11	2.212902073370878E-4	1.5438851674680545E-5	0.0	0.0	1.0807196172276382E-4
12	2.26436491228648E-4	1.5438851674680545E-5	0.0	0.0	1.13218245614324E-4
13	2.3158277512020818E-4	1.5438851674680545E-5	0.0	0.0	1.3894966507212492E-4
14	2.4187534290332855E-4	1.5438851674680545E-5	0.0	0.0	1.4924223285524526E-4
15	2.521679106864489E-4	1.5438851674680545E-5	0.0	0.0	1.6468108452992583E-4
16	2.6760676236112945E-4	2.058513556624073E-5	0.0	0.0	1.955587878792869E-4
17	2.8304561403581E-4	3.087770334936109E-5	0.0	5.146283891560182E-6	2.0585135566240727E-4
18	3.2936216905985166E-4	3.087770334936109E-5	0.0	5.146283891560182E-6	2.0585135566240727E-4
19	3.705324401923331E-4	3.602398724092127E-5	0.0	5.146283891560182E-6	2.26436491228648E-4
20	4.1170271132481454E-4	3.602398724092127E-5	0.0	5.146283891560182E-6	2.3672905901176838E-4
21	4.6831183413197655E-4	3.602398724092127E-5	0.0	5.146283891560182E-6	2.4187534290332855E-4
22	5.146283891560182E-4	3.602398724092127E-5	0.0	5.146283891560182E-6	2.6760676236112945E-4
23	5.918226475294209E-4	3.602398724092127E-5	0.0	5.146283891560182E-6	2.8304561403581E-4
24	6.690169059028236E-4	3.602398724092127E-5	0.0	5.146283891560182E-6	2.9848446571049053E-4
25	7.770888676255875E-4	4.117027113248146E-5	0.0	5.146283891560182E-6	3.139233173851711E-4
26	8.851608293483512E-4	4.117027113248146E-5	0.0	1.0292567783120364E-5	3.1906960127673126E-4
27	9.93232791071115E-4	4.117027113248146E-5	0.0	1.0292567783120364E-5	3.2936216905985166E-4
28	0.001163060159492601	4.117027113248146E-5	0.0	1.0292567783120364E-5	3.2936216905985166E-4
29	0.0012865709728900456	4.117027113248146E-5	0.0	1.5438851674680545E-5	3.345084529514118E-4
30	0.0013689115151550085	4.117027113248146E-5	0.0	1.5438851674680545E-5	3.39654736842972E-4
31	0.0014358132057452908	4.6316555024041634E-5	0.0	1.5438851674680545E-5	3.499473046260924E-4
32	0.0016210794258414572	4.6316555024041634E-5	0.0	2.573141945780091E-5	3.7567872408389327E-4
33	0.001780614226479823	5.146283891560182E-5	0.0	3.087770334936109E-5	3.911175757585738E-4
34	0.0019144176076603876	5.146283891560182E-5	0.0	9.263311004808327E-5	3.96263859650134E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	14585	0.0	23.764141	1
AGCAGTG	23190	0.0	15.101337	2
CAACGCA	23565	0.0	14.513049	13
TCAACGC	23765	0.0	14.357247	12
ACGCAGA	24525	0.0	14.124363	15
AACGCAG	24250	0.0	14.094845	14
AGTGGTA	24625	0.0	14.018275	5
GCAGTGG	25580	0.0	13.760751	3
ATCAACG	24950	0.0	13.747495	11
GGTATCA	24960	0.0	13.63782	8
GTATCAA	25155	0.0	13.571855	9
CGCAGAG	26380	0.0	13.229718	16
TGGTATC	26240	0.0	13.170732	7
GTGGTAT	26110	0.0	13.167369	6
AGAGTAC	25960	0.0	13.135592	19
TATCAAC	26225	0.0	13.033365	10
CAGTGGT	27265	0.0	12.763617	4
CAGAGTA	28405	0.0	12.159832	18
GAGTACT	8695	0.0	12.075906	20
GCAGAGT	29555	0.0	11.774658	17
>>END_MODULE