FastQCFastQC Report
Thu 26 May 2016
SRR805248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14438052
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT527920.3656448944774544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT275650.032.7444231
TCAACGC351950.025.63432312
CAACGCA351350.025.61548413
ACGCAGA360600.025.16361815
AACGCAG357600.025.15659914
AGCAGTG362400.025.1490082
GTGGTAT358950.025.1177046
AGTGGTA360100.025.0597065
ATCAACG361200.025.0387611
GTATCAA357850.025.0216569
GGTATCA359700.024.8596048
GCAGTGG372900.024.521323
TATCAAC366750.024.44171710
TGGTATC369900.024.3903737
CGCAGAG373600.024.26659616
AGAGTAC370650.023.99028819
CAGTGGT379050.023.886034
CAGAGTA385850.023.07632418
GCAGAGT394550.022.76517717
GAGTACT126600.022.51184820
Produced by FastQC (version 0.11.5)