##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805248_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14438052
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.85682195908423	37.0	35.0	37.0	35.0	37.0
2	35.913028987567024	37.0	36.0	37.0	35.0	37.0
3	37.71679759845719	39.0	38.0	39.0	35.0	39.0
4	37.74720544017988	39.0	38.0	39.0	35.0	39.0
5	37.741584806593025	39.0	38.0	39.0	35.0	39.0
6	37.716660530104754	39.0	38.0	39.0	35.0	39.0
7	37.72278317047203	39.0	38.0	39.0	35.0	39.0
8	38.642480509143475	40.0	39.0	40.0	36.0	40.0
9	38.667273950807214	40.0	39.0	40.0	36.0	40.0
10	38.41237412083015	40.0	39.0	40.0	35.0	40.0
11	38.53345278158023	40.0	39.0	40.0	36.0	40.0
12	38.58745840505353	40.0	39.0	40.0	36.0	40.0
13	38.67500553398755	40.0	39.0	40.0	36.0	40.0
14	38.38410410213234	40.0	39.0	40.0	35.0	40.0
15	38.496504445336534	40.0	39.0	40.0	35.0	40.0
16	38.560337364070996	40.0	39.0	40.0	36.0	40.0
17	38.54430985565089	40.0	39.0	40.0	36.0	40.0
18	38.47758312547981	40.0	39.0	40.0	35.0	40.0
19	38.408471793840334	40.0	39.0	40.0	35.0	40.0
20	38.30014256770927	40.0	39.0	40.0	35.0	40.0
21	38.3020656110672	40.0	38.0	40.0	35.0	40.0
22	38.211368195654096	40.0	38.0	40.0	34.0	40.0
23	38.18204969756308	40.0	38.0	40.0	34.0	40.0
24	38.12198030593047	40.0	38.0	40.0	34.0	40.0
25	38.09926456837806	40.0	38.0	40.0	34.0	40.0
26	38.09118113717834	40.0	38.0	40.0	34.0	40.0
27	37.94545358335044	40.0	38.0	40.0	34.0	40.0
28	37.854398986788524	40.0	38.0	40.0	33.0	40.0
29	37.71895744661399	40.0	38.0	40.0	33.0	40.0
30	37.657534340505215	40.0	38.0	40.0	33.0	40.0
31	37.50331076519187	40.0	38.0	40.0	33.0	40.0
32	37.467613567259626	40.0	38.0	40.0	32.0	40.0
33	37.530719241072134	40.0	38.0	40.0	33.0	40.0
34	37.52745398063395	40.0	38.0	40.0	33.0	40.0
35	37.475529385820195	40.0	38.0	40.0	33.0	40.0
36	37.59219637108939	40.0	38.0	40.0	33.0	40.0
37	37.552350483292344	40.0	38.0	40.0	33.0	40.0
38	37.42731193931148	40.0	38.0	40.0	33.0	40.0
39	37.33930657681521	40.0	37.0	40.0	32.0	40.0
40	36.698946298295645	40.0	37.0	40.0	30.0	40.0
41	36.80807182298554	40.0	37.0	40.0	30.0	40.0
42	36.95787104797794	40.0	37.0	40.0	31.0	40.0
43	34.49013675806127	39.0	33.0	40.0	19.0	40.0
44	35.60071836560777	39.0	34.0	40.0	26.0	40.0
45	31.897802210436698	38.0	28.0	40.0	10.0	40.0
46	33.55198963128821	38.0	31.0	40.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	27.0
11	54.0
12	122.0
13	262.0
14	443.0
15	904.0
16	1504.0
17	2439.0
18	3608.0
19	5425.0
20	8166.0
21	12822.0
22	19566.0
23	29717.0
24	42336.0
25	58879.0
26	77933.0
27	100488.0
28	127471.0
29	156835.0
30	191393.0
31	230371.0
32	277850.0
33	342761.0
34	438594.0
35	599794.0
36	917030.0
37	1590069.0
38	2852835.0
39	6348347.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.607236765735433	32.43344046689955	23.160887632209665	26.79843513515535
2	27.915178585033495	26.190617681665092	25.464938067822445	20.429265665478972
3	22.620475393771958	24.95473766128561	26.2208502919923	26.203936652950134
4	20.97799620059548	29.2054011164387	24.720689466972416	25.095913215993406
5	25.254203267864668	28.71014732458368	24.393727076201138	21.641922331350514
6	25.42847885573483	25.711654176061977	26.217352590224774	22.642514377978415
7	24.17032436231702	26.59561691563377	27.12526592922646	22.108792792822744
8	23.628298332766775	25.788721359363436	26.926298644720216	23.656681663149573
9	23.453711068501484	25.36628902569405	27.929494920782943	23.250504985021525
10	23.00599831611633	26.487132751703623	27.57115710623566	22.935711825944384
11	22.48223652332046	27.858238770715054	26.627366351083925	23.03215835488056
12	22.53345534425281	26.186413513401945	27.63663685378055	23.64349428856469
13	22.75039596754465	26.71702526074847	25.831275576511292	24.701303195195585
14	23.623401550292243	27.896457222899603	25.563815672640605	22.916325554167557
15	22.971727764936713	27.54928434943994	25.733644677273638	23.745343208349716
16	23.459307391329524	26.272567795156853	25.585612241873072	24.68251257164055
17	25.071810241437003	25.870941592397646	25.611363638252584	23.44588452791277
18	23.184588890523457	26.207178087459443	25.618088922245192	24.990144099771907
19	23.69397893843297	27.571877425015508	25.506799670758905	23.227343965792617
20	24.933592149411847	26.184107108078013	25.74316119653815	23.13913954597199
21	23.67475196792476	27.38753122651172	25.687281081963135	23.250435723600386
22	24.706040676401496	25.943146623935142	26.025948652906916	23.324864046756446
23	23.35076781826246	26.211950199375927	27.36608096438495	23.071201017976666
24	23.263373757069168	27.2365828852812	25.82361526333331	23.676428094316325
25	23.24941065456753	26.140382372912914	25.796367820257192	24.813839152262368
26	23.467431756029136	26.761165564440407	26.727137428234776	23.044265251295673
27	23.688015530072892	25.58429627487143	27.429378977163953	23.29830921789172
28	24.63984061007676	25.86278259698746	26.222845020921103	23.274531772014672
29	24.747874574769504	25.772327180979815	26.339238839145334	23.140559405105343
30	24.579347684853886	25.659708110207664	26.296400650170813	23.46454355476764
31	24.390714204381588	26.089142773554215	26.123877376255468	23.39626564580873
32	23.96039992098657	26.208909622987917	26.40761371409384	23.423076741931666
33	23.786636867632836	25.977278652272478	26.456408385286323	23.77967609480836
34	23.738818782478415	26.17876012636608	26.380864953249926	23.701556137905584
35	23.818289337093397	26.090701155529842	26.501435235168845	23.589574272207912
36	23.74979671772896	25.978476874858185	26.47738074360724	23.794345663805615
37	23.800537634855452	26.17716711367988	26.330553456934496	23.691741794530177
38	23.907089405135817	26.13572800541236	26.481148564917206	23.476034024534613
39	23.833263656343668	25.93725940313832	26.510314549358878	23.71916239115914
40	24.177229726004587	25.96005333683519	26.195230492312955	23.66748644484727
41	24.03169070176503	26.0938664024759	26.4269168721653	23.447526023593763
42	23.781615414600253	25.989053093866126	26.51716450390953	23.71216698762409
43	25.612111661600885	26.609240637171826	25.475396542414448	22.303251158812838
44	23.89816160795099	25.98248018500003	26.48236063978714	23.636997567261844
45	24.92410333471579	28.28159228128559	24.915210168241533	21.879094215757082
46	23.83390778756026	26.078573480688394	26.329466052622614	23.758052679128735
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	666.0
1	561.5
2	457.0
3	465.5
4	474.0
5	586.5
6	699.0
7	699.0
8	958.0
9	1217.0
10	1981.5
11	2746.0
12	4820.5
13	6895.0
14	9904.5
15	12914.0
16	18928.5
17	24943.0
18	36670.0
19	48397.0
20	48397.0
21	65738.5
22	83080.0
23	108360.5
24	133641.0
25	195844.5
26	258048.0
27	258078.5
28	258109.0
29	292380.5
30	326652.0
31	326652.0
32	372783.0
33	418914.0
34	471808.5
35	524703.0
36	583240.0
37	641777.0
38	702580.5
39	763384.0
40	828178.5
41	892973.0
42	960757.5
43	1028542.0
44	1028542.0
45	1077661.0
46	1126780.0
47	1160842.5
48	1194905.0
49	1200463.5
50	1206022.0
51	1179981.0
52	1153940.0
53	1107697.0
54	1061454.0
55	992575.5
56	923697.0
57	923697.0
58	835887.5
59	748078.0
60	659303.5
61	570529.0
62	491573.0
63	412617.0
64	343379.5
65	274142.0
66	221334.0
67	168526.0
68	129317.0
69	90108.0
70	90108.0
71	66447.0
72	42786.0
73	31090.5
74	19395.0
75	14067.5
76	8740.0
77	6351.5
78	3963.0
79	2828.5
80	1694.0
81	1694.0
82	1265.0
83	836.0
84	584.5
85	333.0
86	259.0
87	185.0
88	113.0
89	41.0
90	31.0
91	21.0
92	20.0
93	19.0
94	19.0
95	11.0
96	3.0
97	4.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.4438052E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.23744224987616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.39770840024158	36.87313137030388
2	14.062476024371307	14.129256543292435
3	4.708842896377844	7.096806692115646
4	2.1558930824330615	4.332262169025538
5	1.2536617485585857	3.1490379847045364
6	0.8130521105898183	2.4507395071137554
7	0.5568890685887036	1.9583677695968627
8	0.4143786404961892	1.6653858417207603
9	0.3313603184000084	1.49820253735689
>10	2.167411639938662	20.120657998486273
>50	0.11025698279482893	3.654034664080794
>100	0.027534048496826103	2.397472752616074
>500	4.3753087619928565E-4	0.13890525076230525
>1k	6.96485074675478E-5	0.08435991455478066
>5k	0.0	0.0
>10k+	2.7859328797134622E-5	0.4513790042693676
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	52792	0.3656448944774544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.696598959471817E-5	0.0	0.0	0.0	6.926142113908441E-6
2	9.696598959471817E-5	0.0	0.0	6.926142113908441E-6	6.926142113908441E-6
3	9.696598959471817E-5	0.0	0.0	6.926142113908441E-6	6.926142113908441E-6
4	9.696598959471817E-5	6.926142113908441E-6	0.0	2.0778426341725324E-5	1.3852284227816883E-5
5	9.696598959471817E-5	1.3852284227816883E-5	0.0	2.7704568455633766E-5	1.3852284227816883E-5
6	1.1774441593644351E-4	1.3852284227816883E-5	0.0	3.4630710569542204E-5	2.0778426341725324E-5
7	1.2467055805035195E-4	1.3852284227816883E-5	0.0	3.4630710569542204E-5	2.0778426341725324E-5
8	1.2467055805035195E-4	2.0778426341725324E-5	0.0	3.4630710569542204E-5	2.7704568455633766E-5
9	1.4544898439207727E-4	2.7704568455633766E-5	0.0	3.4630710569542204E-5	2.7704568455633766E-5
10	1.523751265059857E-4	3.4630710569542204E-5	0.0	3.4630710569542204E-5	3.4630710569542204E-5
11	1.5930126861989416E-4	3.4630710569542204E-5	0.0	3.4630710569542204E-5	3.4630710569542204E-5
12	1.662274107338026E-4	3.4630710569542204E-5	0.0	3.4630710569542204E-5	4.155685268345065E-5
13	1.662274107338026E-4	3.4630710569542204E-5	0.0	3.4630710569542204E-5	4.8482994797359086E-5
14	1.7315355284771102E-4	3.4630710569542204E-5	0.0	3.4630710569542204E-5	7.618756325299285E-5
15	1.8007969496161948E-4	3.4630710569542204E-5	0.0	3.4630710569542204E-5	8.31137053669013E-5
16	1.9393197918943635E-4	3.4630710569542204E-5	0.0	3.4630710569542204E-5	8.31137053669013E-5
17	2.008581213033448E-4	3.4630710569542204E-5	0.0	3.4630710569542204E-5	9.696598959471817E-5
18	2.2163654764507012E-4	3.4630710569542204E-5	0.0	5.540913691126753E-5	9.696598959471817E-5
19	2.3548883187288701E-4	3.4630710569542204E-5	0.0	5.540913691126753E-5	1.1081827382253506E-4
20	2.97824110898063E-4	3.4630710569542204E-5	0.0	5.540913691126753E-5	1.1081827382253506E-4
21	3.2552867935369673E-4	3.4630710569542204E-5	0.0	5.540913691126753E-5	1.1774441593644351E-4
22	3.6015938992323897E-4	3.4630710569542204E-5	0.0	5.540913691126753E-5	1.3159670016426038E-4
23	4.017162426066896E-4	3.4630710569542204E-5	0.0	5.540913691126753E-5	1.3852284227816881E-4
24	4.501992374040487E-4	3.4630710569542204E-5	0.0	5.540913691126753E-5	1.3852284227816881E-4
25	4.848299479735909E-4	3.4630710569542204E-5	0.0	6.926142113908441E-5	1.3852284227816881E-4
26	5.471652269987669E-4	3.4630710569542204E-5	0.0	6.926142113908441E-5	1.4544898439207727E-4
27	6.095005060239428E-4	4.155685268345065E-5	0.0	6.926142113908441E-5	1.4544898439207727E-4
28	6.57983500821302E-4	4.155685268345065E-5	0.0	6.926142113908441E-5	1.4544898439207727E-4
29	7.341710640742947E-4	4.155685268345065E-5	0.0	6.926142113908441E-5	1.4544898439207727E-4
30	8.103586273272876E-4	4.8482994797359086E-5	6.926142113908441E-6	6.926142113908441E-5	1.5930126861989416E-4
31	9.419553274915481E-4	4.8482994797359086E-5	6.926142113908441E-6	8.31137053669013E-5	1.5930126861989416E-4
32	0.001052773601314083	4.8482994797359086E-5	6.926142113908441E-6	1.1081827382253506E-4	1.5930126861989416E-4
33	0.001191296443592252	4.8482994797359086E-5	6.926142113908441E-6	1.1081827382253506E-4	1.8700583707552791E-4
34	0.0013644499964399629	4.8482994797359086E-5	6.926142113908441E-6	1.3852284227816881E-4	1.8700583707552791E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	27565	0.0	32.744423	1
TCAACGC	35195	0.0	25.634323	12
CAACGCA	35135	0.0	25.615484	13
ACGCAGA	36060	0.0	25.163618	15
AACGCAG	35760	0.0	25.156599	14
AGCAGTG	36240	0.0	25.149008	2
GTGGTAT	35895	0.0	25.117704	6
AGTGGTA	36010	0.0	25.059706	5
ATCAACG	36120	0.0	25.03876	11
GTATCAA	35785	0.0	25.021656	9
GGTATCA	35970	0.0	24.859604	8
GCAGTGG	37290	0.0	24.52132	3
TATCAAC	36675	0.0	24.441717	10
TGGTATC	36990	0.0	24.390373	7
CGCAGAG	37360	0.0	24.266596	16
AGAGTAC	37065	0.0	23.990288	19
CAGTGGT	37905	0.0	23.88603	4
CAGAGTA	38585	0.0	23.076324	18
GCAGAGT	39455	0.0	22.765177	17
GAGTACT	12660	0.0	22.511848	20
>>END_MODULE