FastQCFastQC Report
Thu 26 May 2016
SRR805247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25269417
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT3051381.2075387413963685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC1317700.033.3414319
ACATGGG885450.032.24349224
GTACATG882500.032.1654422
GAGTACT469600.032.0741120
GAGTACA900800.031.84280820
AGTACAT897300.031.76195321
GTACTTT470000.031.72340422
TACATGG896400.031.58634823
CATGGGG564800.030.98087925
CAGAGTA1468400.030.5638818
AGTACTT493500.030.39108521
TACTTTT509800.029.27814923
ACTTTTT537150.028.28632724
ATGGGGG269950.027.63474826
CAACGCA1705900.026.9171713
TCAACGC1711850.026.82478112
ATCAACG1732100.026.54234711
CGCAGAG1818800.026.01715516
ACGCAGA1764400.026.0145115
AACGCAG1771300.025.93462414
Produced by FastQC (version 0.11.5)