##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805247_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25269417
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.325661886065674	35.0	35.0	37.0	35.0	37.0
2	36.149861035575135	37.0	35.0	37.0	35.0	37.0
3	38.26558863625544	39.0	39.0	39.0	37.0	39.0
4	38.20611892233208	39.0	39.0	39.0	37.0	39.0
5	38.19359184266103	39.0	39.0	39.0	37.0	39.0
6	38.22733330175366	39.0	39.0	39.0	37.0	39.0
7	38.20104618955	39.0	39.0	39.0	37.0	39.0
8	39.189859465297516	40.0	40.0	40.0	38.0	40.0
9	38.1982585510382	40.0	40.0	40.0	36.0	40.0
10	38.26664944426696	40.0	39.0	40.0	36.0	40.0
11	38.85910917533238	40.0	39.0	40.0	36.0	40.0
12	39.05037096819448	40.0	40.0	40.0	37.0	40.0
13	39.13920301366668	40.0	40.0	40.0	38.0	40.0
14	39.14555128042725	40.0	40.0	40.0	38.0	40.0
15	38.13841316560647	40.0	40.0	40.0	36.0	40.0
16	38.26571938719441	40.0	40.0	40.0	36.0	40.0
17	38.50635042351789	40.0	39.0	40.0	34.0	40.0
18	38.81638543540597	40.0	39.0	40.0	36.0	40.0
19	38.90539488900753	40.0	39.0	40.0	37.0	40.0
20	38.908996673726186	40.0	40.0	40.0	37.0	40.0
21	38.87352217108926	40.0	40.0	40.0	37.0	40.0
22	38.86505114067333	40.0	40.0	40.0	37.0	40.0
23	38.24730107544626	40.0	39.0	40.0	35.0	40.0
24	38.525161225524116	40.0	39.0	40.0	36.0	40.0
25	38.63794946278341	40.0	39.0	40.0	36.0	40.0
26	38.514681719803825	40.0	39.0	40.0	36.0	40.0
27	38.51582262463752	40.0	39.0	40.0	35.0	40.0
28	38.4741778173988	40.0	39.0	40.0	35.0	40.0
29	38.45147859960521	40.0	39.0	40.0	35.0	40.0
30	38.50632228673894	40.0	39.0	40.0	35.0	40.0
31	38.50621911854951	40.0	39.0	40.0	35.0	40.0
32	38.488580247023506	40.0	39.0	40.0	35.0	40.0
33	38.50489538401302	40.0	39.0	40.0	35.0	40.0
34	38.45985817559622	40.0	39.0	40.0	35.0	40.0
35	38.44503622699329	40.0	39.0	40.0	35.0	40.0
36	38.40990569746821	40.0	39.0	40.0	35.0	40.0
37	37.94686561229331	40.0	38.0	40.0	34.0	40.0
38	37.95577472167245	40.0	38.0	40.0	34.0	40.0
39	38.03009198827183	40.0	38.0	40.0	34.0	40.0
40	37.99693301194879	40.0	38.0	40.0	34.0	40.0
41	37.73394748284062	40.0	38.0	40.0	34.0	40.0
42	37.81046911371165	40.0	38.0	40.0	34.0	40.0
43	35.933456240798904	40.0	36.0	40.0	27.0	40.0
44	36.37841328116117	40.0	36.0	40.0	28.0	40.0
45	34.56624163509589	39.0	34.0	40.0	21.0	40.0
46	35.43306456179816	39.0	35.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	21.0
13	61.0
14	221.0
15	596.0
16	1183.0
17	2376.0
18	3343.0
19	4985.0
20	7587.0
21	12408.0
22	19911.0
23	30103.0
24	44146.0
25	61093.0
26	81530.0
27	103631.0
28	130704.0
29	163476.0
30	201137.0
31	253580.0
32	338912.0
33	545246.0
34	820999.0
35	676015.0
36	897194.0
37	1851556.0
38	4737400.0
39	1.4279997E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.878107476717805	42.94881832849567	34.51245828109133	13.660615913695198
2	32.69238463237992	43.10893678314779	13.369445761253612	10.829232823218677
3	36.162801065018634	25.615521719397012	21.09669170444257	17.124985511141787
4	23.902332214470956	26.9654460172152	20.14695471605063	28.985267052263215
5	20.141592502905787	33.58489829820767	24.41740939254752	21.85609980633902
6	27.020219738350114	25.24956551233453	22.898494254932753	24.831720494382598
7	23.590730249138712	24.55171799175264	29.150027481837036	22.707524277271613
8	25.208567336555486	26.666788553135202	25.663029740654487	22.46161436965483
9	28.46976248007621	24.534115686167198	25.11133517643086	21.88478665732573
10	22.293470403373377	26.629340122884514	29.520309075591257	21.556880398150856
11	21.80252120577218	30.96171945716041	25.80844267202524	21.427316665042174
12	21.908087551050347	25.75694168171747	29.80267807524012	22.532292691992062
13	21.828342933277803	26.677366557368536	25.43954219442419	26.054748314929466
14	22.250948646737676	30.783856232219364	25.097899963422186	21.867295157620774
15	23.412380269794113	30.39416777996896	24.29434759021152	21.8991043600254
16	22.763220061626274	25.841296615588718	24.931366639760626	26.464116683024386
17	27.028795322028998	25.247460200605342	25.314711455353322	22.40903302201234
18	22.300740060603694	25.67724850953229	25.033252646865577	26.988758782998435
19	22.591273079232497	30.424920369156123	24.900847534393055	22.08295901721832
20	27.291915757296657	26.036845250525566	25.01715017801954	21.654088814158236
21	23.108752370503836	29.92287475409504	24.76135480292244	22.20701807247868
22	26.964393361350602	25.98104657499617	25.053894199458576	22.00066586419465
23	22.746167828090375	25.53231837521222	29.869529637347785	21.85198415934962
24	22.54453278443266	29.58384041863728	25.42609115200402	22.445535644926036
25	22.61151889653806	26.36536885674885	24.671657442670718	26.351454804042373
26	22.681049586541707	28.610165402707942	26.616170052518424	22.09261495823192
27	23.008144588377323	25.279969854468742	29.458297356049012	22.253588201104918
28	25.584286333159174	25.963289932648625	26.273748223000158	22.178675511192047
29	25.56570656141374	25.78935635911189	26.32128790308063	22.323649176393744
30	25.992016357164076	25.14095200534306	26.325933835355205	22.541097802137656
31	24.530035655353664	26.19636218754077	26.71131273032536	22.562289426780207
32	23.901576360071942	26.54127714936993	26.733062341723198	22.824084148834935
33	24.005623081846327	26.021300768434823	27.013096503176154	22.959979646542696
34	23.62842007791474	26.30500339600237	27.15708083015924	22.909495695923653
35	23.20909105263489	26.446142386268747	27.124990655700525	23.21977590539584
36	23.774604693096006	25.855871546225224	27.375981804408074	22.993541956270697
37	23.497835347764454	26.386172660809702	27.13761461136994	22.9783773800559
38	23.560282376122885	26.613368246683333	27.253565050590602	22.57278432660318
39	23.93357155806167	26.176777248165244	26.91678244891839	22.9728687448547
40	23.407801612518405	26.262730952597757	27.058887033286126	23.27058040159771
41	23.828788768652636	26.282177384622685	26.981208153714032	22.907825693010647
42	23.738383042236393	26.322332644239477	26.77441272190807	23.164871591616063
43	25.287564014634768	26.44640752891133	26.424365073400786	21.84166338305312
44	23.70539059132231	26.176931584927342	27.135600318756858	22.982077504993487
45	24.80689206244845	27.953145891731495	25.66059201128384	21.579370034536215
46	23.694468297388894	25.979586311785507	26.975493736163365	23.350451654662237
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3311.0
1	2682.0
2	2053.0
3	2027.0
4	2001.0
5	2337.0
6	2673.0
7	2673.0
8	3435.5
9	4198.0
10	6370.5
11	8543.0
12	13219.0
13	17895.0
14	23780.0
15	29665.0
16	41580.5
17	53496.0
18	76371.0
19	99246.0
20	99246.0
21	131182.5
22	163119.0
23	210167.0
24	257215.0
25	478469.5
26	699724.0
27	598890.5
28	498057.0
29	548496.0
30	598935.0
31	598935.0
32	678610.5
33	758286.0
34	841497.5
35	924709.0
36	1023118.0
37	1121527.0
38	1230176.5
39	1338826.0
40	1473052.5
41	1607279.0
42	1755153.5
43	1903028.0
44	1903028.0
45	2024141.0
46	2145254.0
47	2189280.5
48	2233307.0
49	2159791.5
50	2086276.0
51	2009428.0
52	1932580.0
53	1835115.5
54	1737651.0
55	1619708.5
56	1501766.0
57	1501766.0
58	1333290.0
59	1164814.0
60	1021980.0
61	879146.0
62	746255.5
63	613365.0
64	505616.5
65	397868.0
66	321337.0
67	244806.0
68	187061.5
69	129317.0
70	129317.0
71	94334.0
72	59351.0
73	43054.5
74	26758.0
75	19570.0
76	12382.0
77	8954.5
78	5527.0
79	4158.5
80	2790.0
81	2790.0
82	2202.5
83	1615.0
84	1153.5
85	692.0
86	455.0
87	218.0
88	143.0
89	68.0
90	53.0
91	38.0
92	28.5
93	19.0
94	19.0
95	16.5
96	14.0
97	10.0
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2.5269417E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.33350916511641
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.52510916610133	27.49716209427419
2	16.878175160474136	13.952684153717959
3	6.2386867114240525	7.736004430948082
4	3.005874174980864	4.96973311043033
5	1.7085653404283203	3.5310500578897113
6	1.1300539362393975	2.8025456838376215
7	0.7863926427947976	2.2753057255837215
8	0.5727827629268297	1.8940097264845457
9	0.4407938627737116	1.6397601450195847
>10	2.4274722205848245	18.49010572844977
>50	0.19211720156330844	5.468276118999755
>100	0.08941492562342888	6.349548794618879
>500	0.0031826393139859858	0.8888142871618632
>1k	0.001298962571318013	0.8961032185101017
>5k	2.007304994256061E-5	0.05275166437108887
>10k+	6.021914982236798E-5	1.5561450597028557
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	305138	1.2075387413963685	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5829411497700956E-4	1.5829411497700955E-5	0.0	0.0	0.0
2	0.002101354376319802	1.5829411497700955E-5	0.0	0.0	0.0
3	0.002121141140691928	1.5829411497700955E-5	0.0	0.0	0.0
4	0.00239419848902727	1.9786764372126195E-5	0.0	0.0	0.0
5	0.0024139852533993957	2.7701470120976675E-5	0.0	0.0	0.0
6	0.002433772017771522	3.165882299540191E-5	0.0	0.0	3.957352874425239E-6
7	0.0025050043695111763	3.165882299540191E-5	0.0	0.0	3.957352874425239E-6
8	0.00272661613047899	3.561617586982715E-5	0.0	3.957352874425239E-6	7.914705748850478E-6
9	0.0038861205226855848	4.748823449310287E-5	0.0	7.914705748850478E-6	1.5829411497700955E-5
10	0.006078494015117167	4.748823449310287E-5	0.0	7.914705748850478E-6	1.5829411497700955E-5
11	0.009897339538937523	6.331764599080382E-5	0.0	7.914705748850478E-6	1.9786764372126195E-5
12	0.015045855628564758	6.331764599080382E-5	0.0	7.914705748850478E-6	2.3744117246551435E-5
13	0.02079588935510463	6.727499886522906E-5	0.0	1.1872058623275717E-5	3.165882299540191E-5
14	0.029161733331639586	7.518970461407954E-5	0.0	1.1872058623275717E-5	3.561617586982715E-5
15	0.04137016694924145	7.518970461407954E-5	0.0	1.1872058623275717E-5	3.561617586982715E-5
16	0.052818788814953666	7.518970461407954E-5	0.0	1.1872058623275717E-5	3.957352874425239E-5
17	0.0675955444480575	7.518970461407954E-5	0.0	1.1872058623275717E-5	3.957352874425239E-5
18	0.0858468559049067	7.518970461407954E-5	0.0	1.1872058623275717E-5	3.957352874425239E-5
19	0.10297427914541915	7.518970461407954E-5	0.0	1.5829411497700955E-5	5.540294024195335E-5
20	0.12703894197479904	7.914705748850478E-5	0.0	1.5829411497700955E-5	6.727499886522906E-5
21	0.15145580921000273	8.706176323735526E-5	0.0	1.5829411497700955E-5	6.727499886522906E-5
22	0.17404042206434758	8.706176323735526E-5	0.0	1.9786764372126195E-5	6.727499886522906E-5
23	0.2021890730601343	8.706176323735526E-5	0.0	1.9786764372126195E-5	7.518970461407954E-5
24	0.22943149024767764	8.706176323735526E-5	0.0	2.3744117246551435E-5	7.914705748850478E-5
25	0.2647825234749183	9.497646898620574E-5	0.0	2.7701470120976675E-5	7.914705748850478E-5
26	0.30196185373014345	9.893382186063098E-5	0.0	3.165882299540191E-5	7.914705748850478E-5
27	0.3319031855780448	9.893382186063098E-5	0.0	3.165882299540191E-5	8.310441036293002E-5
28	0.36948220847358687	1.0684852760948146E-4	0.0	3.165882299540191E-5	9.893382186063098E-5
29	0.40729867254159446	1.0684852760948146E-4	0.0	3.165882299540191E-5	9.893382186063098E-5
30	0.4397252219946349	1.1476323335833194E-4	0.0	3.561617586982715E-5	9.893382186063098E-5
31	0.47742296547640967	1.2267793910718242E-4	0.0	3.561617586982715E-5	1.0289117473505622E-4
32	0.5147487177879886	1.2663529198160764E-4	0.0	3.957352874425239E-5	1.108058804839067E-4
33	0.5498702245485125	1.2663529198160764E-4	0.0	3.957352874425239E-5	1.1476323335833194E-4
34	0.5934011061671902	1.2663529198160764E-4	0.0	6.727499886522906E-5	1.1476323335833194E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGTAC	131770	0.0	33.34143	19
ACATGGG	88545	0.0	32.243492	24
GTACATG	88250	0.0	32.16544	22
GAGTACT	46960	0.0	32.07411	20
GAGTACA	90080	0.0	31.842808	20
AGTACAT	89730	0.0	31.761953	21
GTACTTT	47000	0.0	31.723404	22
TACATGG	89640	0.0	31.586348	23
CATGGGG	56480	0.0	30.980879	25
CAGAGTA	146840	0.0	30.56388	18
AGTACTT	49350	0.0	30.391085	21
TACTTTT	50980	0.0	29.278149	23
ACTTTTT	53715	0.0	28.286327	24
ATGGGGG	26995	0.0	27.634748	26
CAACGCA	170590	0.0	26.91717	13
TCAACGC	171185	0.0	26.824781	12
ATCAACG	173210	0.0	26.542347	11
CGCAGAG	181880	0.0	26.017155	16
ACGCAGA	176440	0.0	26.01451	15
AACGCAG	177130	0.0	25.934624	14
>>END_MODULE