FastQCFastQC Report
Thu 26 May 2016
SRR805246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26480853
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT3606351.361870782636798No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2489800.9402265100750342No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC949590.3585949440525953No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC804230.3037024524851975No Hit
TAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG591030.22319145081920133No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT588190.22211897781389445No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG547520.20676071122029188No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA539530.20374343681451654No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT495570.18714276311265352No Hit
TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG487050.18392534409673283No Hit
TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT429210.16208314739710236No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG422130.15940951751063304No Hit
AGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA418230.15793675528503556No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC384180.1450784081615498No Hit
TGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC381510.14407013248402534No Hit
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA380290.14360942224935125No Hit
TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC373520.14105285807824997No Hit
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA343200.1296030758525792No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG303060.11444495386912197No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC300360.11342534925140063No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC278060.10500417037170215No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG269560.10179430398257941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC1604850.034.90793619
GAGTACT516650.034.0888420
GTACATG1108900.034.06619322
ACATGGG1115250.034.06590324
GTACTTT515800.033.89298222
AGTACAT1121600.033.7910121
GAGTACA1131150.033.75679420
TACATGG1124100.033.5592923
CATGGGG699000.033.0472125
AGTACTT545100.032.23628621
TACTTTT543650.032.1088923
ACTTTTT566200.031.31755424
ATGGGGG305000.029.91475526
CTTTTTT624250.028.4661625
ATGGGGA275350.027.35427726
ATGGGGC160300.025.78914626
CATGGGC231400.025.5056225
CATGGGA232450.025.21832725
TGGGGGG173250.024.92352127
CATGGGT137100.024.72647725
Produced by FastQC (version 0.11.5)