##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805246_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26480853
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.62108373170607	37.0	37.0	37.0	35.0	37.0
2	36.55829009737715	37.0	37.0	37.0	35.0	37.0
3	38.38617215238497	39.0	39.0	39.0	37.0	39.0
4	38.31170838794355	39.0	39.0	39.0	37.0	39.0
5	38.29654025872958	39.0	39.0	39.0	37.0	39.0
6	38.29565773428824	39.0	39.0	39.0	37.0	39.0
7	38.25042078516126	39.0	39.0	39.0	37.0	39.0
8	39.247538060801894	40.0	40.0	40.0	38.0	40.0
9	38.2838319067743	40.0	40.0	40.0	36.0	40.0
10	38.30502144322919	40.0	39.0	40.0	36.0	40.0
11	38.917239486205375	40.0	40.0	40.0	36.0	40.0
12	39.116919836381406	40.0	40.0	40.0	37.0	40.0
13	39.2243823490127	40.0	40.0	40.0	38.0	40.0
14	39.23685222677683	40.0	40.0	40.0	38.0	40.0
15	38.251490199352716	40.0	40.0	40.0	36.0	40.0
16	38.38148699364027	40.0	40.0	40.0	36.0	40.0
17	38.62332425620882	40.0	40.0	40.0	35.0	40.0
18	38.92872767353831	40.0	40.0	40.0	37.0	40.0
19	39.020668707310904	40.0	40.0	40.0	37.0	40.0
20	39.01499075577362	40.0	40.0	40.0	37.0	40.0
21	38.98522970540262	40.0	40.0	40.0	37.0	40.0
22	38.99597074912957	40.0	40.0	40.0	37.0	40.0
23	38.33411688060048	40.0	39.0	40.0	36.0	40.0
24	38.64526524126696	40.0	39.0	40.0	36.0	40.0
25	38.791483567391126	40.0	39.0	40.0	37.0	40.0
26	38.67376062243916	40.0	39.0	40.0	36.0	40.0
27	38.679392049795375	40.0	39.0	40.0	36.0	40.0
28	38.647086632745555	40.0	39.0	40.0	36.0	40.0
29	38.61914387727616	40.0	39.0	40.0	36.0	40.0
30	38.61818087959629	40.0	39.0	40.0	36.0	40.0
31	38.625746270333515	40.0	39.0	40.0	36.0	40.0
32	38.60740596233815	40.0	39.0	40.0	36.0	40.0
33	38.54900501128117	40.0	39.0	40.0	35.0	40.0
34	38.54656320927426	40.0	39.0	40.0	35.0	40.0
35	38.547230295036194	40.0	39.0	40.0	35.0	40.0
36	38.52047398171048	40.0	39.0	40.0	35.0	40.0
37	38.10805229725795	40.0	38.0	40.0	35.0	40.0
38	38.08420076196186	40.0	38.0	40.0	35.0	40.0
39	38.158493648221985	40.0	38.0	40.0	35.0	40.0
40	38.124398522962984	40.0	38.0	40.0	35.0	40.0
41	37.876819526923846	40.0	38.0	40.0	34.0	40.0
42	37.952340017143705	40.0	38.0	40.0	34.0	40.0
43	36.111116511239274	40.0	36.0	40.0	28.0	40.0
44	36.57103511733553	40.0	37.0	40.0	30.0	40.0
45	34.75188012259273	39.0	35.0	40.0	22.0	40.0
46	35.564635814412775	39.0	35.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	20.0
13	51.0
14	213.0
15	635.0
16	1418.0
17	2700.0
18	4008.0
19	5600.0
20	8042.0
21	12253.0
22	18970.0
23	27787.0
24	39928.0
25	55959.0
26	74628.0
27	96042.0
28	120671.0
29	151581.0
30	187883.0
31	234777.0
32	313850.0
33	512644.0
34	822886.0
35	659705.0
36	832000.0
37	1740576.0
38	4721582.0
39	1.5834442E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.6201128415312	35.77952719272298	31.240742131682843	21.359617834062973
2	29.950221014406143	41.87065273161707	12.670940018435207	15.508186235541583
3	32.926420459340946	26.777132141475956	18.721828938063286	21.57461846111981
4	23.19233447653669	24.62114796679699	20.48443832228516	31.70207923438116
5	21.524672940105063	31.918416676381234	23.595380405608534	22.96152997790517
6	28.57695709424466	24.73626132813773	22.731197518448518	23.95558405916909
7	23.8194290795693	23.44735269668239	29.85514477196033	22.878073451787976
8	28.918520864867908	26.200708111630693	23.638728707115288	21.242042316386105
9	30.894110548478178	23.857105358350804	23.03434862917747	22.21443546399355
10	21.85692054557306	25.516485439498492	30.824728342398938	21.801865672529505
11	22.51351193256501	32.0078208961018	24.434269545622264	21.04439762571092
12	21.242559671321764	25.24146408727846	30.68046939424497	22.83550684715481
13	22.377723255364923	25.151376354832678	24.505513474207195	27.965386915595204
14	22.873220889070303	31.2771306875953	23.75363814753248	22.096010275801916
15	22.89938318829835	31.860344528931904	23.33063817846049	21.90963410430925
16	22.959672031712874	24.769609951764014	23.76148155046214	28.509236466060965
17	28.98208377199934	24.104446333356407	24.723104652255724	22.19036524238853
18	22.22603629875518	24.54738901348835	24.006281066550237	29.220293621206235
19	22.765097483831052	31.30705797128212	23.883350736473634	22.044493808413197
20	29.341483070805914	25.226064281237466	24.323181734364827	21.109270913591793
21	22.240952736681102	31.225901220024898	23.85510768856275	22.678038354731246
22	29.15903426524818	24.681912625699784	24.646018766842595	21.513034342209444
23	22.79364641312725	24.372976202843617	30.24133701433258	22.59204036969655
24	21.20149226310799	31.33317495474938	24.927527825482056	22.537804956660572
25	22.919533596595247	27.014552741182467	23.305869338876658	26.760044323345628
26	23.419755398362735	28.47720955212432	25.303497587483303	22.79953746202964
27	23.035277602273613	24.20996030603697	29.40746281851268	23.347299273176738
28	26.39367017369116	25.75993303538976	25.46684957618246	22.379547214736625
29	26.95897296057646	25.15952941546105	25.699629086721643	22.181868537240852
30	26.76237430871279	24.17961762787626	26.645958119249407	22.41204994416154
31	26.220239204530156	25.801695285268945	25.974627781061283	22.003437729139616
32	25.56677460503255	25.266908886960703	25.803300218463505	23.36301628954324
33	23.38998671983867	25.62158779401857	28.111129954914972	22.87729553122779
34	23.781254327419134	26.776622339167094	27.08338738181886	22.358735951594912
35	22.778144646624487	25.878898236397447	27.72782281597953	23.615134300998537
36	23.858842462514332	25.41063159861202	26.86285823194593	23.86766770692772
37	23.931483627056878	26.28298642796741	26.490336244077938	23.295193700897777
38	23.06754997658119	27.467778322699804	26.512710900966823	22.951960799752182
39	24.117036562228567	25.293022094114566	26.36907126821028	24.220870075446587
40	24.421667987809908	24.995036980115408	27.217907217716892	23.365387814357792
41	23.871413809819494	25.423316235319156	26.234498563924657	24.470771390936687
42	23.917511267480695	26.715151509658696	26.347383900360004	23.0199533225006
43	26.50591731316208	25.866715849372373	26.28531641333457	21.342050424130974
44	23.409245918173408	26.64071281993824	26.240578428496995	23.709462833391356
45	25.96436753755629	27.381285640609843	25.516738452496224	21.13760836933765
46	23.49917126914303	24.935186189055162	27.469798650368247	24.095843891433557
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4453.0
1	3103.0
2	1753.0
3	1641.5
4	1530.0
5	1728.0
6	1926.0
7	1926.0
8	2505.0
9	3084.0
10	4289.5
11	5495.0
12	8374.5
13	11254.0
14	14657.5
15	18061.0
16	25501.5
17	32942.0
18	49417.0
19	65892.0
20	65892.0
21	89358.0
22	112824.0
23	152704.5
24	192585.0
25	425247.5
26	657910.0
27	529745.5
28	401581.0
29	441783.5
30	481986.0
31	481986.0
32	555021.0
33	628056.0
34	712800.0
35	797544.0
36	894563.0
37	991582.0
38	1094713.5
39	1197845.0
40	1306045.0
41	1414245.0
42	1542752.5
43	1671260.0
44	1671260.0
45	1888287.0
46	2105314.0
47	2946016.0
48	3786718.0
49	3113933.5
50	2441149.0
51	2232684.5
52	2024220.0
53	1928477.5
54	1832735.0
55	1720003.0
56	1607271.0
57	1607271.0
58	1440665.0
59	1274059.0
60	1125186.0
61	976313.0
62	836818.0
63	697323.0
64	579607.0
65	461891.0
66	373790.0
67	285689.0
68	220660.0
69	155631.0
70	155631.0
71	114682.5
72	73734.0
73	54235.5
74	34737.0
75	25469.0
76	16201.0
77	11787.5
78	7374.0
79	5506.0
80	3638.0
81	3638.0
82	2702.0
83	1766.0
84	1268.5
85	771.0
86	583.0
87	395.0
88	230.0
89	65.0
90	46.0
91	27.0
92	20.0
93	13.0
94	13.0
95	9.0
96	5.0
97	4.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2.6480853E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.39989223043003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.77621109370001	25.34822990691256
2	15.9565632067631	11.935474886019696
3	5.976736259899606	6.70587876029846
4	2.817848666692019	4.215489458237698
5	1.6492181838866276	3.08402911709127
6	1.0469230089208375	2.349288462431811
7	0.7421959955706563	1.9430635173739486
8	0.5529223093234826	1.6543387824398996
9	0.41786052955847425	1.406514489555362
>10	2.7361447198382347	19.209857691220915
>50	0.21844277692767816	5.613042502378915
>100	0.10264160114277594	6.588868766588864
>500	0.0039462589171123455	1.0086809663583176
>1k	0.0017786176885742431	1.189629963535486
>5k	1.1545338666929968E-4	0.32051707397710544
>10k+	4.513177842498538E-4	7.427095655579535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	360635	1.361870782636798	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	248980	0.9402265100750342	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	94959	0.3585949440525953	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	80423	0.3037024524851975	No Hit
TAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	59103	0.22319145081920133	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	58819	0.22211897781389445	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	54752	0.20676071122029188	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA	53953	0.20374343681451654	No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	49557	0.18714276311265352	No Hit
TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	48705	0.18392534409673283	No Hit
TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	42921	0.16208314739710236	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	42213	0.15940951751063304	No Hit
AGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	41823	0.15793675528503556	No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	38418	0.1450784081615498	No Hit
TGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	38151	0.14407013248402534	No Hit
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	38029	0.14360942224935125	No Hit
TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	37352	0.14105285807824997	No Hit
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA	34320	0.1296030758525792	No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG	30306	0.11444495386912197	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	30036	0.11342534925140063	No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	27806	0.10500417037170215	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG	26956	0.10179430398257941	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7944719152362654E-4	1.888156699483963E-5	0.0	0.0	3.776313398967926E-6
2	0.010732282679866846	2.2657880393807556E-5	0.0	3.776313398967926E-6	3.776313398967926E-6
3	0.010762493187058589	2.6434193792775482E-5	0.0	3.776313398967926E-6	3.776313398967926E-6
4	0.011208098168136804	3.0210507191743408E-5	0.0	7.552626797935852E-6	3.776313398967926E-6
5	0.01121565079493474	3.0210507191743408E-5	0.0	7.552626797935852E-6	7.552626797935852E-6
6	0.011219427108333709	3.0210507191743408E-5	0.0	2.6434193792775482E-5	7.552626797935852E-6
7	0.011219427108333709	3.0210507191743408E-5	0.0	3.0210507191743408E-5	7.552626797935852E-6
8	0.011245861302126483	3.398682059071133E-5	0.0	3.0210507191743408E-5	7.552626797935852E-6
9	0.011328940196903777	3.398682059071133E-5	0.0	3.776313398967926E-5	7.552626797935852E-6
10	0.011521532180251141	3.776313398967926E-5	3.776313398967926E-6	4.531576078761511E-5	1.1328940196903778E-5
11	0.011861400386158256	4.153944738864718E-5	3.776313398967926E-6	4.9092074186583035E-5	3.0210507191743408E-5
12	0.01252603154437661	4.153944738864718E-5	3.776313398967926E-6	5.6644700984518887E-5	4.153944738864718E-5
13	0.0130924785542218	4.153944738864718E-5	3.776313398967926E-6	6.797364118142266E-5	4.153944738864718E-5
14	0.013934596442191647	4.153944738864718E-5	3.776313398967926E-6	7.17499545803906E-5	4.531576078761511E-5
15	0.015124135162866544	4.153944738864718E-5	3.776313398967926E-6	7.552626797935852E-5	5.2868387585550964E-5
16	0.01622304236196621	4.153944738864718E-5	3.776313398967926E-6	8.307889477729437E-5	6.419732778245474E-5
17	0.01769958090096267	4.531576078761511E-5	3.776313398967926E-6	9.440783497419815E-5	7.552626797935852E-5
18	0.019606619167441473	4.531576078761511E-5	3.776313398967926E-6	9.440783497419815E-5	7.930258137832644E-5
19	0.021389039091754332	4.9092074186583035E-5	3.776313398967926E-6	9.440783497419815E-5	8.307889477729437E-5
20	0.023987142710244266	4.9092074186583035E-5	3.776313398967926E-6	9.440783497419815E-5	9.063152157523022E-5
21	0.02687602246045473	4.9092074186583035E-5	3.776313398967926E-6	9.818414837316607E-5	9.440783497419815E-5
22	0.029636507555100285	5.6644700984518887E-5	3.776313398967926E-6	1.0573677517110193E-4	1.0573677517110193E-4
23	0.03314847901614045	5.6644700984518887E-5	3.776313398967926E-6	1.0951308857006985E-4	1.1706571536800571E-4
24	0.0366868846709734	5.6644700984518887E-5	3.776313398967926E-6	1.1328940196903777E-4	1.2084202876697363E-4
25	0.04100321088599374	5.6644700984518887E-5	3.776313398967926E-6	1.2084202876697363E-4	1.2084202876697363E-4
26	0.045576326412143896	6.0421014383486816E-5	3.776313398967926E-6	1.3594728236284532E-4	1.2084202876697363E-4
27	0.0498851000003663	6.0421014383486816E-5	3.776313398967926E-6	1.4727622255974912E-4	1.2084202876697363E-4
28	0.05486983368700397	6.0421014383486816E-5	3.776313398967926E-6	1.623814761556208E-4	1.2461834216594155E-4
29	0.06002827778999415	6.0421014383486816E-5	3.776313398967926E-6	1.623814761556208E-4	1.2461834216594155E-4
30	0.06526980078776164	6.0421014383486816E-5	3.776313398967926E-6	1.6615778955458873E-4	1.2461834216594155E-4
31	0.07071524470907338	6.797364118142266E-5	3.776313398967926E-6	1.6993410295355668E-4	1.2839465556490948E-4
32	0.07653454365688296	7.17499545803906E-5	3.776313398967926E-6	1.8503935654942837E-4	1.321709689638774E-4
33	0.08179494822164528	7.17499545803906E-5	3.776313398967926E-6	1.8503935654942837E-4	1.321709689638774E-4
34	0.08781061546620118	7.552626797935852E-5	3.776313398967926E-6	2.2280249053910762E-4	1.3594728236284532E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGTAC	160485	0.0	34.907936	19
GAGTACT	51665	0.0	34.08884	20
GTACATG	110890	0.0	34.066193	22
ACATGGG	111525	0.0	34.065903	24
GTACTTT	51580	0.0	33.892982	22
AGTACAT	112160	0.0	33.79101	21
GAGTACA	113115	0.0	33.756794	20
TACATGG	112410	0.0	33.55929	23
CATGGGG	69900	0.0	33.04721	25
AGTACTT	54510	0.0	32.236286	21
TACTTTT	54365	0.0	32.10889	23
ACTTTTT	56620	0.0	31.317554	24
ATGGGGG	30500	0.0	29.914755	26
CTTTTTT	62425	0.0	28.46616	25
ATGGGGA	27535	0.0	27.354277	26
ATGGGGC	16030	0.0	25.789146	26
CATGGGC	23140	0.0	25.50562	25
CATGGGA	23245	0.0	25.218327	25
TGGGGGG	17325	0.0	24.923521	27
CATGGGT	13710	0.0	24.726477	25
>>END_MODULE