FastQCFastQC Report
Thu 26 May 2016
SRR805245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22015925
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT220280.10005484666213207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT149900.019.6130751
AGCAGTG300900.015.0415421
GCAGTGG308750.014.6720652
TCAACGC303300.014.09825111
CAACGCA302750.014.07762112
AGTGGTA315850.013.7660294
AACGCAG310550.013.7562413
GGTATCA314800.013.7420587
ACGCAGA311100.013.73834714
GTGGTAT319500.013.53990655
GTATCAA320450.013.5122498
ATCAACG322200.013.38919810
TGGTATC327700.013.2255126
CAGTGGT335700.013.029493
TATCAAC331650.013.0257819
CGCAGAG333400.012.86742715
AGAGTAC341050.012.73127118
CAGAGTA359900.012.17004817
GCAGAGT372450.011.958652516
GTACATG275100.011.42857121
Produced by FastQC (version 0.11.5)