##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805245_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22015925
Sequences flagged as poor quality	0
Sequence length	46
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.579745207162546	37.0	37.0	37.0	35.0	37.0
2	36.510028354475224	37.0	37.0	37.0	35.0	37.0
3	38.3647803578546	39.0	39.0	39.0	37.0	39.0
4	38.327680712938474	39.0	39.0	39.0	37.0	39.0
5	38.33509012226377	39.0	39.0	39.0	37.0	39.0
6	38.31685559430276	39.0	39.0	39.0	37.0	39.0
7	38.31795747850703	39.0	39.0	39.0	37.0	39.0
8	39.28861181167723	40.0	40.0	40.0	38.0	40.0
9	38.27982439983784	40.0	40.0	40.0	36.0	40.0
10	38.4795730363362	40.0	40.0	40.0	36.0	40.0
11	39.00403889457291	40.0	40.0	40.0	36.0	40.0
12	39.15002603797024	40.0	40.0	40.0	37.0	40.0
13	39.23497223032873	40.0	40.0	40.0	38.0	40.0
14	39.234088097592995	40.0	40.0	40.0	38.0	40.0
15	38.25222188029801	40.0	40.0	40.0	36.0	40.0
16	38.38957872539991	40.0	40.0	40.0	36.0	40.0
17	38.64591490023699	40.0	40.0	40.0	35.0	40.0
18	38.942330154195204	40.0	40.0	40.0	37.0	40.0
19	39.02541096955954	40.0	40.0	40.0	37.0	40.0
20	39.025907064999544	40.0	40.0	40.0	37.0	40.0
21	39.02502052491549	40.0	40.0	40.0	37.0	40.0
22	39.01178147182097	40.0	40.0	40.0	37.0	40.0
23	38.5494257452276	40.0	40.0	40.0	36.0	40.0
24	38.759408609903964	40.0	40.0	40.0	36.0	40.0
25	38.824953527957604	40.0	40.0	40.0	37.0	40.0
26	38.693358739185385	40.0	40.0	40.0	36.0	40.0
27	38.69898121473433	40.0	39.0	40.0	36.0	40.0
28	38.667071358573395	40.0	39.0	40.0	36.0	40.0
29	38.651103144655515	40.0	39.0	40.0	36.0	40.0
30	38.65927341231404	40.0	39.0	40.0	36.0	40.0
31	38.665175594484445	40.0	39.0	40.0	36.0	40.0
32	38.64507019350766	40.0	39.0	40.0	36.0	40.0
33	38.677318622769654	40.0	39.0	40.0	36.0	40.0
34	38.63814534251911	40.0	39.0	40.0	36.0	40.0
35	38.6225840158885	40.0	39.0	40.0	36.0	40.0
36	38.601537023768024	40.0	39.0	40.0	36.0	40.0
37	38.20919434454832	40.0	39.0	40.0	35.0	40.0
38	38.234701471775544	40.0	39.0	40.0	35.0	40.0
39	38.31660418537945	40.0	39.0	40.0	35.0	40.0
40	38.298191786172964	40.0	39.0	40.0	35.0	40.0
41	38.0529444481665	40.0	38.0	40.0	35.0	40.0
42	38.139664311174755	40.0	38.0	40.0	35.0	40.0
43	36.31142820481084	40.0	37.0	40.0	28.0	40.0
44	36.78020523779946	40.0	37.0	40.0	30.0	40.0
45	34.96201177102484	39.0	35.0	40.0	22.0	40.0
46	35.88609290774746	39.0	35.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	10.0
13	30.0
14	131.0
15	447.0
16	1013.0
17	1892.0
18	2775.0
19	4042.0
20	5601.0
21	8598.0
22	13235.0
23	20804.0
24	30671.0
25	42529.0
26	57407.0
27	75040.0
28	95458.0
29	119986.0
30	149971.0
31	188597.0
32	249799.0
33	412767.0
34	698206.0
35	547094.0
36	662819.0
37	1336642.0
38	3549644.0
39	1.3740715E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.826727698245703	27.04435539274412	28.303321345798555	30.82559556321163
2	25.34940957511438	24.73674851272431	30.339261239307458	19.574580672853855
3	15.869271902043636	25.478970336245236	33.263757938855626	25.3879998228555
4	17.246988259634787	28.635644425569218	32.28482564325596	21.83254167154003
5	23.53717593060478	27.766419080733606	29.543346463980054	19.153058524681565
6	21.754343730731275	26.924837362045885	30.35141153505928	20.96940737216356
7	23.380162314324746	27.54194974773942	28.83649449205518	20.24139344588065
8	21.2967204421345	25.92189971577392	29.665539830827004	23.115840011264574
9	22.755514474181755	24.889805901864218	30.55064004805612	21.804039575897903
10	22.062002845667397	26.794036589423335	28.376459312974582	22.76750125193468
11	22.019865165783404	27.159394847139062	28.380238395615905	22.440501591461633
12	22.049920682415117	26.922766134059778	28.104556133798603	22.922757049726506
13	22.354622846871074	27.324825098195966	27.232709958813906	23.08784209611906
14	22.775077585883853	27.502260295672336	27.152081958854783	22.570580159589024
15	23.99110189555969	26.84138413443905	26.502265973380634	22.66524799662063
16	23.036688215462217	27.026772665695397	26.836964606302026	23.099574512540354
17	23.64002420974817	26.436354593322786	26.974524122879235	22.949097074049806
18	22.704083521360104	27.05718701349137	26.896076362905486	23.342653102243034
19	23.382378891643206	26.98275452882402	26.729410642523536	22.905455937009233
20	23.308359744139754	26.87666768486902	26.960139081142398	22.854833489848826
21	23.236298270456498	26.995790547069902	26.971330979733988	22.79658020273961
22	23.11758874541951	26.538453414971208	27.228840032839862	23.115117806769415
23	23.282169611315446	26.92029065324305	27.23960496776765	22.557934767673853
24	22.834811619316472	26.82992879018256	27.0443281397443	23.29093145075667
25	22.89102547360604	26.77496403171795	26.95579222767156	23.378218267004453
26	23.310290164960136	26.534778802162524	27.468289431400223	22.686641601477113
27	23.278054408343053	26.284128420677305	27.556366584642706	22.881450586336936
28	23.478591065331116	26.414606699468678	27.001831628696042	23.104970606504153
29	23.630835406643147	26.401393536723983	27.080947087165313	22.886823969467557
30	23.336048791954006	26.3486408134112	27.154725499837053	23.160584894797744
31	23.215985701259427	26.557303406511423	26.929129709517085	23.29758118271206
32	23.408114807803894	26.609833563659034	26.98616115380117	22.9958904747359
33	23.139459277772794	26.524858710229072	27.040562683602893	23.29511932839524
34	23.184049727640332	26.588885091132898	26.855937236341422	23.37112794488535
35	23.349684376195867	26.633257517001894	26.906882177332996	23.110175929469236
36	23.20421240533841	26.50410555086829	26.99075328427036	23.300928759522936
37	23.31686268008271	26.802435055533664	26.53214888768017	23.348553376703453
38	23.596192301708875	26.685133602153893	26.76215057963724	22.95652351649999
39	23.195995625893527	26.618899728264882	26.90883530898656	23.276269336855027
40	23.30413552916809	26.66013805915491	26.719236189258456	23.316490222418544
41	23.6907874640743	26.615711127286275	26.774160068223342	22.919341340416086
42	23.18295506548101	26.520988784255035	27.014454309778035	23.281601840485923
43	24.99063745902114	26.663717286464227	25.981184074709557	22.364461179805073
44	23.43651697578003	26.51825894210668	26.859670897316374	23.185553184796916
45	24.20098633148505	28.434589961584628	25.713573243004777	21.650850463925543
46	23.339632561429966	26.527652142710334	26.735488061482766	23.39722723437693
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3881.0
1	3383.5
2	2886.0
3	2764.0
4	2642.0
5	2925.0
6	3208.0
7	3208.0
8	4216.0
9	5224.0
10	7406.0
11	9588.0
12	14903.5
13	20219.0
14	27597.0
15	34975.0
16	48761.0
17	62547.0
18	88063.0
19	113579.0
20	113579.0
21	152164.0
22	190749.0
23	241598.0
24	292447.0
25	365660.0
26	438873.0
27	475351.5
28	511830.0
29	577999.5
30	644169.0
31	644169.0
32	728248.0
33	812327.0
34	898039.5
35	983752.0
36	1070071.0
37	1156390.0
38	1240062.0
39	1323734.0
40	1403563.5
41	1483393.0
42	1557351.5
43	1631310.0
44	1631310.0
45	1671305.0
46	1711300.0
47	1733980.0
48	1756660.0
49	1736430.0
50	1716200.0
51	1658068.0
52	1599936.0
53	1509880.0
54	1419824.0
55	1309501.5
56	1199179.0
57	1199179.0
58	1076524.0
59	953869.0
60	831992.0
61	710115.0
62	608012.5
63	505910.0
64	417795.5
65	329681.0
66	264312.5
67	198944.0
68	150578.0
69	102212.0
70	102212.0
71	74482.5
72	46753.0
73	33752.0
74	20751.0
75	15076.0
76	9401.0
77	6731.5
78	4062.0
79	2958.5
80	1855.0
81	1855.0
82	1423.5
83	992.0
84	656.5
85	321.0
86	244.5
87	168.0
88	101.5
89	35.0
90	24.5
91	14.0
92	11.0
93	8.0
94	8.0
95	7.0
96	6.0
97	4.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2.2015925E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.83586138502441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.17842525652448	30.32495860379279
2	16.093722706533324	14.109643954652293
3	5.677201170894493	7.4659501074668775
4	2.626799890622846	4.605921435661614
5	1.4376861463757424	3.1511105313848478
6	0.9392509978691017	2.470372592900148
7	0.6308540138592474	1.9357820374004167
8	0.4980084433378498	1.7464503272583813
9	0.36684233940386407	1.4472764946239276
>10	2.271695864750771	19.1167347756755
>50	0.1812532279849126	5.459222498418422
>100	0.09562759715405503	6.977478154933641
>500	0.002006249843199034	0.6045101150337091
>1k	5.937882096293197E-4	0.3741473720220244
>5k	1.0768878752824062E-5	0.029768297590275456
>10k+	2.1537757505647717E-5	0.18067270118499698
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	22028	0.10005484666213207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2256616517361865E-4	0.0	0.0	0.0	9.084333272392598E-6
2	2.9069866471656314E-4	4.542166636196299E-6	0.0	1.3626499908588896E-5	9.084333272392598E-6
3	3.0432516462515204E-4	9.084333272392598E-6	0.0	2.7252999817177793E-5	2.7252999817177793E-5
4	3.088673312613483E-4	9.084333272392598E-6	0.0	4.542166636196299E-5	3.179516645337409E-5
5	0.0010719513261423265	9.084333272392598E-6	0.0	4.996383299815929E-5	4.087949972576669E-5
6	0.0010901199926871116	9.084333272392598E-6	0.0	4.996383299815929E-5	4.996383299815929E-5
7	0.0011355416590490748	9.084333272392598E-6	0.0	5.4505999634355585E-5	4.996383299815929E-5
8	0.0011491681589576635	9.084333272392598E-6	0.0	5.9048166270551887E-5	7.267466617914078E-5
9	0.0011673368255024489	9.084333272392598E-6	0.0	6.359033290674818E-5	7.267466617914078E-5
10	0.0012581801582263747	9.084333272392598E-6	0.0	7.721683281533708E-5	7.267466617914078E-5
11	0.0014080716572208527	9.084333272392598E-6	0.0	9.992766599631858E-5	7.267466617914078E-5
12	0.0015715896561239195	1.3626499908588896E-5	0.0	1.0901199926871117E-4	7.721683281533708E-5
13	0.0017623606548441638	1.3626499908588896E-5	0.0	1.1355416590490746E-4	9.538549936012227E-5
14	0.0019213364871110345	1.3626499908588896E-5	0.0	1.1809633254110377E-4	1.0901199926871117E-4
15	0.0020939388192864937	1.8168666544785196E-5	0.0	1.2718066581349636E-4	1.1809633254110377E-4
16	0.0021847821520104196	1.8168666544785196E-5	0.0	1.2718066581349636E-4	1.3626499908588895E-4
17	0.002275625484734346	2.2710833180981494E-5	0.0	1.4989149899447785E-4	1.6806016553926306E-4
18	0.002443685650273609	3.179516645337409E-5	0.0	1.6351799890306675E-4	1.6806016553926306E-4
19	0.0025754084827233014	3.633733308957039E-5	0.0	1.8622883208404826E-4	1.8622883208404826E-4
20	0.0026662518154472272	3.633733308957039E-5	0.0	1.9077099872024454E-4	1.9531316535644085E-4
21	0.0027798059813521347	5.4505999634355585E-5	0.0	1.9531316535644085E-4	1.9985533199263716E-4
22	0.0028842758139846496	5.4505999634355585E-5	0.0	2.2256616517361865E-4	1.9985533199263716E-4
23	0.003011456479798146	5.4505999634355585E-5	0.0	2.3165049844601124E-4	2.1348183190122603E-4
24	0.0031658901454288202	6.359033290674818E-5	0.0	2.3165049844601124E-4	2.1348183190122603E-4
25	0.003315781644423298	6.813249954294448E-5	0.0	2.3619266508220755E-4	2.1802399853742234E-4
26	0.0035338056429607204	7.267466617914078E-5	0.0	2.4073483171840383E-4	2.3165049844601124E-4
27	0.00374274530822575	7.721683281533708E-5	0.0	2.4527699835460016E-4	2.3165049844601124E-4
28	0.0038790103073116393	7.721683281533708E-5	0.0	2.679878315355816E-4	2.3619266508220755E-4
29	0.004087949972576669	7.721683281533708E-5	0.0	2.679878315355816E-4	2.3619266508220755E-4
30	0.004228757138298754	8.175899945153337E-5	0.0	2.679878315355816E-4	2.4073483171840383E-4
31	0.004378648637293232	8.175899945153337E-5	0.0	2.7707216480797424E-4	2.4527699835460016E-4
32	0.0046420943021926175	8.175899945153337E-5	0.0	3.134094978975446E-4	2.725299981717779E-4
33	0.0049009978004558065	8.175899945153337E-5	0.0	3.134094978975446E-4	2.997829979889557E-4
34	0.005114479632357032	8.630116608772968E-5	0.0	3.3157816444232983E-4	2.997829979889557E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	14990	0.0	19.613075	1
AGCAGTG	30090	0.0	15.041542	1
GCAGTGG	30875	0.0	14.672065	2
TCAACGC	30330	0.0	14.098251	11
CAACGCA	30275	0.0	14.077621	12
AGTGGTA	31585	0.0	13.766029	4
AACGCAG	31055	0.0	13.75624	13
GGTATCA	31480	0.0	13.742058	7
ACGCAGA	31110	0.0	13.738347	14
GTGGTAT	31950	0.0	13.5399065	5
GTATCAA	32045	0.0	13.512249	8
ATCAACG	32220	0.0	13.389198	10
TGGTATC	32770	0.0	13.225512	6
CAGTGGT	33570	0.0	13.02949	3
TATCAAC	33165	0.0	13.025781	9
CGCAGAG	33340	0.0	12.867427	15
AGAGTAC	34105	0.0	12.731271	18
CAGAGTA	35990	0.0	12.170048	17
GCAGAGT	37245	0.0	11.9586525	16
GTACATG	27510	0.0	11.428571	21
>>END_MODULE