FastQCFastQC Report
Thu 26 May 2016
SRR805244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21974683
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT4749292.161255295468881No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC380890.1733312830951873No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC352630.16047102932042295No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG261450.11897782552767655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2054550.038.3675231
AGCAGTG2158500.036.569842
TCAACGC2098900.036.26661312
AGTGGTA2149700.036.2404065
CAACGCA2101250.036.23652613
GCAGTGG2153800.036.203923
GTGGTAT2152200.036.1815836
AACGCAG2110200.036.07620214
ACGCAGA2109900.036.0538415
ATCAACG2116250.035.99669311
GGTATCA2165900.035.9961248
GTATCAA2121150.035.9880269
TGGTATC2169350.035.9056857
CGCAGAG2184850.035.8834716
TATCAAC2134750.035.85056710
CAGTGGT2180350.035.7208674
AGAGTAC2148550.035.7040819
GAGTACT649650.035.48064420
CAGAGTA2175200.035.3907718
GCAGAGT2186000.035.22598317
Produced by FastQC (version 0.11.5)