##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805244_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21974683
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.64279407352543	37.0	37.0	37.0	35.0	37.0
2	36.572263226732325	37.0	37.0	37.0	35.0	37.0
3	38.42069212101945	39.0	39.0	39.0	37.0	39.0
4	38.357909190316875	39.0	39.0	39.0	37.0	39.0
5	38.345656863400485	39.0	39.0	39.0	37.0	39.0
6	38.348961484450086	39.0	39.0	39.0	37.0	39.0
7	38.286052317569265	39.0	39.0	39.0	37.0	39.0
8	39.29365943526921	40.0	40.0	40.0	38.0	40.0
9	38.30764634921013	40.0	40.0	40.0	37.0	40.0
10	38.28874227673728	40.0	39.0	40.0	36.0	40.0
11	38.958651872247714	40.0	40.0	40.0	36.0	40.0
12	39.178466055687814	40.0	40.0	40.0	38.0	40.0
13	39.2833199004509	40.0	40.0	40.0	38.0	40.0
14	39.29475219278476	40.0	40.0	40.0	38.0	40.0
15	38.292140551015	40.0	40.0	40.0	37.0	40.0
16	38.427951201844415	40.0	40.0	40.0	36.0	40.0
17	38.6916434698967	40.0	40.0	40.0	35.0	40.0
18	39.00330216367626	40.0	40.0	40.0	37.0	40.0
19	39.0871562970897	40.0	40.0	40.0	37.0	40.0
20	39.100242993266384	40.0	40.0	40.0	38.0	40.0
21	39.058559161012695	40.0	40.0	40.0	38.0	40.0
22	39.076087741516	40.0	40.0	40.0	38.0	40.0
23	38.32904265331154	40.0	39.0	40.0	36.0	40.0
24	38.70035467633367	40.0	39.0	40.0	36.0	40.0
25	38.85990036807357	40.0	40.0	40.0	37.0	40.0
26	38.754608064198244	40.0	40.0	40.0	37.0	40.0
27	38.782165185272525	40.0	40.0	40.0	37.0	40.0
28	38.75731044675366	40.0	40.0	40.0	36.0	40.0
29	38.74395357603111	40.0	40.0	40.0	36.0	40.0
30	38.73647560695187	40.0	40.0	40.0	36.0	40.0
31	38.70653019203963	40.0	40.0	40.0	36.0	40.0
32	38.67835404042006	40.0	39.0	40.0	36.0	40.0
33	38.66530192949769	40.0	39.0	40.0	36.0	40.0
34	38.63017828289036	40.0	39.0	40.0	35.0	40.0
35	38.61774324571599	40.0	39.0	40.0	35.0	40.0
36	38.570424064820415	40.0	39.0	40.0	35.0	40.0
37	38.12992082752684	40.0	38.0	40.0	35.0	40.0
38	38.09955875131396	40.0	38.0	40.0	35.0	40.0
39	38.16626310377264	40.0	38.0	40.0	35.0	40.0
40	38.14410451336204	40.0	38.0	40.0	35.0	40.0
41	37.89352692823828	40.0	38.0	40.0	34.0	40.0
42	37.97115844629022	40.0	38.0	40.0	35.0	40.0
43	36.12887366793869	40.0	36.0	40.0	28.0	40.0
44	36.59135801867995	40.0	36.0	40.0	30.0	40.0
45	34.76414899819033	39.0	35.0	40.0	22.0	40.0
46	35.671682135300884	39.0	35.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	11.0
13	31.0
14	112.0
15	350.0
16	799.0
17	1617.0
18	2458.0
19	3554.0
20	5292.0
21	8320.0
22	13306.0
23	20362.0
24	30098.0
25	41973.0
26	56220.0
27	73299.0
28	93004.0
29	116203.0
30	145416.0
31	181920.0
32	247025.0
33	407415.0
34	676964.0
35	537327.0
36	675184.0
37	1478043.0
38	3929560.0
39	1.3228819E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.917229204171	35.290857210545425	28.66882766864032	22.123085916643255
2	26.21646009637545	37.97317121707739	17.978612023663775	17.831756662883375
3	32.93620208309717	24.875212079282328	20.651369578346134	21.537216259274366
4	21.642564764187952	24.107728880548585	21.82514305212048	32.42456330314298
5	22.023462181456726	33.19932305735651	23.525254038931983	21.251960722254786
6	31.42534979913021	23.785621844920357	22.603111043740654	22.18591731220878
7	22.65918921333245	23.379927710447518	32.644411753289	21.31647132293103
8	30.488198623843633	24.630944619314874	23.63543537806666	21.245421378774836
9	31.749809542189983	23.84257374725269	23.65748802838248	20.750128682174847
10	21.298719075947535	24.592400263521437	32.66014804400136	21.448732616529668
11	21.20345035238961	33.45545872038291	24.002653417116417	21.338437510111067
12	21.153096952524866	24.405835569960214	32.772541019135524	21.6685264583794
13	21.14152909509548	24.600623362803457	23.90787616822504	30.349971373876016
14	21.616284521601518	33.52163032340444	23.643226161669773	21.21885899332427
15	22.942478851685824	32.68403917362539	23.08942522629337	21.284056748395415
16	21.634105028955368	24.226938791335463	23.806140912248882	30.332815267460283
17	30.79279459913028	23.906301629015537	23.891075015735154	21.409828756119033
18	21.479822939880407	24.315809242845503	23.775050588898143	30.429317228375947
19	21.81507237214753	33.076668273212405	23.76212662544438	21.346132729195684
20	30.687118444438994	24.266534356832363	23.846282560708612	21.200064638020034
21	21.765183142801195	33.05146199378621	23.802213665607827	21.381141197804766
22	30.341511638643432	23.975362921048735	24.196376348182135	21.4867490921257
23	21.961672894211944	24.283162583050686	32.50519245260558	21.249972070131797
24	21.644239418607313	32.7579424012624	23.878801801145435	21.719016378984854
25	21.5152318693289	24.181791382383082	23.971908946308805	30.331067801979213
26	22.06150141050954	30.069867219472517	26.598440578187184	21.27019079183076
27	22.050352216684992	23.87999408228096	32.67627114347907	21.393382557554983
28	28.16234937268492	23.926024325356593	26.514657799614223	21.396968502344265
29	28.2527488564909	24.00810969605341	26.393504743617918	21.345636703837776
30	28.054830187994067	23.896954508968342	26.426215113091732	21.622000189945858
31	25.45809648312105	24.91363356640913	27.12895107519867	22.499318875271147
32	24.169017591744097	26.02584073681518	27.41602233806968	22.389119333371042
33	23.938452263452447	25.474874882154158	27.650574071990025	22.93609878240337
34	23.39563214632038	25.280592216051534	28.099936640724238	23.223838996903844
35	23.266720161560464	25.50951019407197	28.097256283514987	23.126513360852577
36	23.32310322747318	25.515412440761942	28.065556167522416	23.09592816424246
37	23.678170920599857	25.52067303997059	27.95278548500563	22.848370554423923
38	23.91482507392712	25.4150924497978	27.987875866059138	22.682206610215946
39	23.76467501260428	25.325548495966927	27.98809884993563	22.92167764149317
40	23.311640035945	25.643828400163954	27.917030703014014	23.12750086087704
41	23.907707792644835	25.862611988532443	27.54649976065639	22.683180458166337
42	23.540940272039418	25.81423358871662	27.808801610471466	22.836024528772498
43	25.202079138069934	25.651032144582018	27.350383165936908	21.79650555141114
44	23.626661645130444	25.390646135828217	27.853375632312876	23.129316586728464
45	24.501058786604567	27.296170779801464	26.54232145237317	21.6604489812208
46	23.47579712526456	25.305593714366665	27.76640736979004	23.45220179057873
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2569.0
1	1739.5
2	910.0
3	885.0
4	860.0
5	1010.0
6	1160.0
7	1160.0
8	1626.0
9	2092.0
10	3294.0
11	4496.0
12	7482.0
13	10468.0
14	14278.0
15	18088.0
16	26228.5
17	34369.0
18	50853.0
19	67337.0
20	67337.0
21	91503.5
22	115670.0
23	155056.5
24	194443.0
25	485446.5
26	776450.0
27	591692.5
28	406935.0
29	436399.5
30	465864.0
31	465864.0
32	525109.5
33	584355.0
34	656573.0
35	728791.0
36	811580.0
37	894369.0
38	983901.0
39	1073433.0
40	1168566.5
41	1263700.0
42	1373679.0
43	1483658.0
44	1483658.0
45	1591577.0
46	1699496.0
47	1761605.0
48	1823714.0
49	1855990.5
50	1888267.0
51	1854666.5
52	1821066.0
53	1748284.5
54	1675503.0
55	1587901.5
56	1500300.0
57	1500300.0
58	1315806.0
59	1131312.0
60	989190.0
61	847068.0
62	723753.5
63	600439.0
64	496174.5
65	391910.0
66	316464.0
67	241018.0
68	182464.5
69	123911.0
70	123911.0
71	90345.0
72	56779.0
73	40560.0
74	24341.0
75	17891.5
76	11442.0
77	7941.5
78	4441.0
79	3190.5
80	1940.0
81	1940.0
82	1460.0
83	980.0
84	699.5
85	419.0
86	331.0
87	243.0
88	146.0
89	49.0
90	30.5
91	12.0
92	11.5
93	11.0
94	11.0
95	6.5
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2.1974683E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.99208443693723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.808047136363	30.01193456864213
2	15.490246585747554	13.319159783268752
3	5.32421083460887	6.866967652846818
4	2.5615673572705786	4.405084804587156
5	1.4804126591993616	3.182301302290487
6	0.9211853101105413	2.3762205980603164
7	0.6541139482114903	1.968520546502074
8	0.4903103213251086	1.6863570187768715
9	0.39316991804928286	1.5212874883354612
>10	2.634660021009402	20.6802403186081
>50	0.17898647156619024	5.2857263296211485
>100	0.05904807196335884	4.282282861025761
>500	0.0032548130413163497	0.9530210403164528
>1k	6.665196113566185E-4	0.5169121518456821
>5k	5.455996481747803E-5	0.14355826408895325
>10k+	6.547195778094846E-5	2.800425271183787
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	474929	2.161255295468881	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC	38089	0.1733312830951873	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	35263	0.16047102932042295	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG	26145	0.11897782552767655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5938940734662703E-4	0.0	0.0	4.5506913569583685E-6	0.0
2	3.686059999136279E-4	0.0	0.0	4.5506913569583685E-6	0.0
3	3.7315669127058627E-4	0.0	0.0	9.101382713916737E-6	1.3652074070875107E-5
4	3.7315669127058627E-4	0.0	0.0	9.101382713916737E-6	1.8202765427833474E-5
5	3.7770738262754463E-4	0.0	0.0	1.3652074070875107E-5	2.7304148141750215E-5
6	3.959101480553781E-4	0.0	0.0	2.2753456784791843E-5	4.095622221262532E-5
7	3.959101480553781E-4	0.0	0.0	2.7304148141750215E-5	4.095622221262532E-5
8	4.050115307692948E-4	0.0	0.0	2.7304148141750215E-5	4.095622221262532E-5
9	4.050115307692948E-4	0.0	0.0	2.7304148141750215E-5	4.095622221262532E-5
10	4.095622221262532E-4	0.0	0.0	2.7304148141750215E-5	5.460829628350043E-5
11	4.186636048401699E-4	0.0	0.0	2.7304148141750215E-5	5.460829628350043E-5
12	4.232142961971283E-4	0.0	0.0	3.640553085566695E-5	5.460829628350043E-5
13	4.2776498755408665E-4	0.0	0.0	3.640553085566695E-5	5.460829628350043E-5
14	4.4596775298192016E-4	0.0	0.0	3.640553085566695E-5	7.736175306829228E-5
15	4.550691356958369E-4	0.0	0.0	4.095622221262532E-5	7.736175306829228E-5
16	4.8692397519454545E-4	0.0	0.0	4.095622221262532E-5	8.191244442525064E-5
17	5.278801974071708E-4	0.0	0.0	4.5506913569583685E-5	9.101382713916737E-5
18	5.779378023337129E-4	0.0	0.0	5.460829628350043E-5	9.556451849612575E-5
19	6.143433331893798E-4	0.0	0.0	5.460829628350043E-5	1.0011520985308411E-4
20	6.871543949007137E-4	0.0	0.0	5.460829628350043E-5	1.1376728392395922E-4
21	7.463133825411725E-4	0.0	0.0	5.460829628350043E-5	1.1831797528091759E-4
22	8.100230615385896E-4	0.0	0.0	5.460829628350043E-5	1.2286866663787597E-4
23	8.782834318929652E-4	0.0	0.0	5.460829628350043E-5	1.3652074070875106E-4
24	9.647465676751742E-4	0.0	0.0	5.460829628350043E-5	1.3652074070875106E-4
25	0.0010512097034573833	0.0	0.0	5.9158987640458794E-5	1.3652074070875106E-4
26	0.0011740783700952592	0.0	0.0	6.826037035437553E-5	1.4107143206570943E-4
27	0.00128329496266226	0.0	0.0	6.826037035437553E-5	1.5017281477962619E-4
28	0.0014607719255836365	0.0	0.0	6.826037035437553E-5	1.5017281477962619E-4
29	0.0016746544193606797	0.0	0.0	6.826037035437553E-5	1.5017281477962619E-4
30	0.0018339286168542227	0.0	0.0	7.28110617113339E-5	1.5927419749354292E-4
31	0.0020887673328438914	0.0	0.0	7.28110617113339E-5	1.6837558020745965E-4
32	0.0023254032834057266	0.0	0.0	7.736175306829228E-5	1.72926271564418E-4
33	0.002589343382109312	0.0	0.0	7.736175306829228E-5	1.7747696292137638E-4
34	0.0027577189623167716	9.101382713916737E-6	0.0	1.0466590121004248E-4	1.8202765427833474E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	205455	0.0	38.367523	1
AGCAGTG	215850	0.0	36.56984	2
TCAACGC	209890	0.0	36.266613	12
AGTGGTA	214970	0.0	36.240406	5
CAACGCA	210125	0.0	36.236526	13
GCAGTGG	215380	0.0	36.20392	3
GTGGTAT	215220	0.0	36.181583	6
AACGCAG	211020	0.0	36.076202	14
ACGCAGA	210990	0.0	36.05384	15
ATCAACG	211625	0.0	35.996693	11
GGTATCA	216590	0.0	35.996124	8
GTATCAA	212115	0.0	35.988026	9
TGGTATC	216935	0.0	35.905685	7
CGCAGAG	218485	0.0	35.88347	16
TATCAAC	213475	0.0	35.850567	10
CAGTGGT	218035	0.0	35.720867	4
AGAGTAC	214855	0.0	35.70408	19
GAGTACT	64965	0.0	35.480644	20
CAGAGTA	217520	0.0	35.39077	18
GCAGAGT	218600	0.0	35.225983	17
>>END_MODULE