FastQCFastQC Report
Thu 26 May 2016
SRR805243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20533150
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1502300.7316461429444582No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC259080.12617645125078228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT952100.041.4612921
GTGGTAT1019050.039.466076
CAACGCA1023600.039.17125713
TCAACGC1024500.039.1574912
AGTGGTA1025650.039.122755
GGTATCA1028900.038.9968878
GTATCAA1031250.038.919429
ACGCAGA1035450.038.8205615
AACGCAG1039300.038.7558914
TGGTATC1037150.038.5960547
ATCAACG1040850.038.56722311
TATCAAC1044350.038.43572210
CGCAGAG1051450.038.22535316
CAGTGGT1051900.038.1218724
GCAGTGG1058800.038.0598753
AGCAGTG1062900.038.0147672
AGAGTAC1039150.037.91117519
CAGAGTA1066850.037.0788318
GCAGAGT1083850.036.6251817
GTACATG762450.036.24939322
Produced by FastQC (version 0.11.5)