##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20533150
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.39400165098877	35.0	35.0	37.0	35.0	37.0
2	35.85808295366274	37.0	35.0	37.0	35.0	37.0
3	37.7551118557065	39.0	38.0	39.0	35.0	39.0
4	37.685693378755815	39.0	38.0	39.0	35.0	39.0
5	37.46054185548735	39.0	37.0	39.0	35.0	39.0
6	37.60032109052922	39.0	37.0	39.0	35.0	39.0
7	37.55992363568181	39.0	37.0	39.0	35.0	39.0
8	38.544380331317896	40.0	38.0	40.0	36.0	40.0
9	38.5410763083112	40.0	38.0	40.0	36.0	40.0
10	38.383048825922955	40.0	38.0	40.0	35.0	40.0
11	38.474767534450386	40.0	38.0	40.0	35.0	40.0
12	38.54905560033409	40.0	38.0	40.0	36.0	40.0
13	38.498660069205165	40.0	39.0	40.0	35.0	40.0
14	38.54361371733027	40.0	39.0	40.0	35.0	40.0
15	38.50433815561665	40.0	39.0	40.0	35.0	40.0
16	38.50078224724409	40.0	39.0	40.0	35.0	40.0
17	38.373961131146466	40.0	38.0	40.0	35.0	40.0
18	38.42439528275009	40.0	38.0	40.0	35.0	40.0
19	38.382747508297555	40.0	38.0	40.0	35.0	40.0
20	38.318462486272196	40.0	38.0	40.0	35.0	40.0
21	38.198321543455336	40.0	38.0	40.0	34.0	40.0
22	38.17306735693257	40.0	38.0	40.0	34.0	40.0
23	37.892368340951094	40.0	38.0	40.0	34.0	40.0
24	37.93353572150401	40.0	38.0	40.0	34.0	40.0
25	37.95648826409976	40.0	38.0	40.0	34.0	40.0
26	37.929012207089514	40.0	38.0	40.0	34.0	40.0
27	37.818740621872436	40.0	38.0	40.0	34.0	40.0
28	37.64776758558721	40.0	38.0	40.0	33.0	40.0
29	37.796754370371815	40.0	38.0	40.0	33.0	40.0
30	37.578017401129394	40.0	38.0	40.0	33.0	40.0
31	37.791060602002126	40.0	38.0	40.0	33.0	40.0
32	37.367420196121884	40.0	38.0	40.0	32.0	40.0
33	37.71545973218917	40.0	38.0	40.0	33.0	40.0
34	37.71751626029128	40.0	38.0	40.0	33.0	40.0
35	37.824044825075546	40.0	38.0	40.0	33.0	40.0
36	37.772728685077546	40.0	38.0	40.0	33.0	40.0
37	37.503729189140486	40.0	38.0	40.0	33.0	40.0
38	37.64058992409835	40.0	38.0	40.0	33.0	40.0
39	37.565996060029754	40.0	37.0	40.0	33.0	40.0
40	37.45054338959195	40.0	37.0	40.0	33.0	40.0
41	37.50895624879767	40.0	37.0	40.0	33.0	40.0
42	37.59308006808502	40.0	37.0	40.0	33.0	40.0
43	37.507590506084064	40.0	37.0	40.0	33.0	40.0
44	37.50143163615909	40.0	37.0	40.0	33.0	40.0
45	37.45275288983911	40.0	37.0	40.0	33.0	40.0
46	37.196201556994424	40.0	37.0	40.0	32.0	40.0
47	37.228166404083154	40.0	36.0	40.0	32.0	40.0
48	37.265875182327115	40.0	36.0	40.0	33.0	40.0
49	37.118063034653716	40.0	36.0	40.0	32.0	40.0
50	37.032518780606	39.0	36.0	40.0	32.0	40.0
51	36.89139304003526	39.0	36.0	40.0	32.0	40.0
52	36.706122733238686	39.0	35.0	40.0	31.0	40.0
53	36.151012387285924	38.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	10.0
12	31.0
13	101.0
14	200.0
15	528.0
16	1089.0
17	1881.0
18	3096.0
19	5085.0
20	8033.0
21	13005.0
22	20409.0
23	30890.0
24	45133.0
25	64274.0
26	87998.0
27	117554.0
28	152612.0
29	194141.0
30	243649.0
31	304364.0
32	376399.0
33	471327.0
34	610894.0
35	832711.0
36	1220475.0
37	2047624.0
38	3888720.0
39	9790913.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.810038888334231	41.22218461366132	38.5137789379613	12.453997560043149
2	33.00418591399761	43.44097228140836	13.000620947102611	10.554220857491423
3	36.5897536422809	25.93066821213501	21.395772202511548	16.083805943072544
4	23.954668426422636	27.561265563247723	19.89516464838566	28.58890136194398
5	20.352025870360855	33.46769979277413	24.54950165951157	21.63077267735345
6	26.90642692426637	25.635204535105427	22.553271173687428	24.905097366940776
7	23.645105597533743	24.987403296620343	28.76601495630237	22.601476149543544
8	24.423953460623434	26.76367240291918	25.99287493638336	22.819499200074027
9	26.22630234523198	25.322213104175447	26.065177529994184	22.386307020598398
10	22.506907123359056	26.938165844013216	28.70670111502619	21.84822591760154
11	21.776941190221667	30.676710587513362	25.883417790256246	21.66293043200873
12	21.99436520942963	26.16457776814566	29.668156128017376	22.172900894407334
13	22.06323920099936	26.65692307317679	25.60851598512649	25.671321740697362
14	22.229073473870304	30.581221098565003	25.28058773252034	21.909117695044355
15	22.065849613917006	30.092888816377418	25.24914102317472	22.592120546530854
16	22.583159427559824	26.046466323968804	25.339278191607228	26.031096056864143
17	26.85004979752254	25.686653046415188	25.254576136637585	22.208721019424686
18	22.546384748565128	25.707297711262033	25.25108909251625	26.495228447656594
19	22.583495469521235	30.094218373703015	25.11237194487938	22.209914211896372
20	26.791612587450047	26.361381473373548	25.05617501454964	21.79083092462676
21	23.074788817107944	29.705369122613916	25.009552845033518	22.210289215244615
22	26.352493407002825	26.04713840789163	25.53288219294166	22.067485992163892
23	22.6854281978167	25.964744815091695	29.41242332520826	21.937403661883344
24	22.751730737855613	29.33502166009599	25.246813080311593	22.6664345217368
25	22.349298573282717	26.133583984921945	25.167589970364997	26.349527471430346
26	22.645536607875556	28.64914540633074	26.496762552263046	22.20855543353066
27	23.18505928218515	25.40996388766458	29.14124720269418	22.26372962745609
28	25.735442443073765	25.767619678422456	26.29426074421119	22.202677134292596
29	25.9509183929402	25.88615482768109	26.13860026347638	22.024326515902334
30	26.504700934829778	25.18616968170982	25.98238945315258	22.32673993030782
31	24.494795002228102	26.14051911177778	26.555730611231105	22.808955274763004
32	24.32819124196726	26.56336217287654	26.743290727433443	22.365155857722755
33	23.90838716904128	26.20788334960783	26.707767682990674	23.175961798360213
34	23.476105711982818	26.403094508149017	26.962433917835305	23.15836586203286
35	23.363414770748765	26.574037592868116	27.032223501995556	23.030324134387563
36	23.73768272281652	26.11162924344292	26.951052322707426	23.199635711033135
37	23.82078249075276	26.365754889045274	26.917082863564527	22.89637975663744
38	23.63269639582821	26.365282482229958	27.19210642302813	22.80991469891371
39	23.84329730216747	26.063619074520954	26.993466662445847	23.09961696086572
40	23.641905893640285	26.48061305742178	26.801908133920026	23.07557291501791
41	23.544200475815938	26.57845971027339	26.95101823149395	22.926321582416726
42	23.646795547687518	26.346863486605805	26.74970961591378	23.256631349792897
43	23.617238465603183	26.542892834270436	27.061025707210046	22.77884299291633
44	23.642767914323912	26.408412737451393	26.852582287666532	23.09623706055817
45	23.739694104411647	25.937213725122543	26.829731434290405	23.493360736175404
46	23.67707341542822	26.347832651103214	26.672765747096765	23.302328186371792
47	23.575822511402293	26.421620647586952	26.822659942580657	23.1798968984301
48	23.803215775465528	25.878333329274856	26.859317737414862	23.459133157844754
49	23.36486121223485	26.48457737853179	26.80430912938346	23.346252279849903
50	23.66499538551075	26.352897631391187	26.98164188154277	23.00046510155529
51	23.649264725577908	26.057054080840008	26.95768549881533	23.335995694766755
52	23.62265409837263	26.391966162035533	26.66300591969571	23.322373819896118
53	23.592278827164854	26.414217984089145	26.60780737490351	23.385695813842496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2634.0
1	1950.5
2	1267.0
3	1191.5
4	1116.0
5	1205.5
6	1295.0
7	1483.5
8	2074.5
9	2477.0
10	3775.0
11	5073.0
12	7467.0
13	9861.0
14	12524.5
15	15188.0
16	21676.0
17	28164.0
18	39640.5
19	51117.0
20	69259.0
21	87401.0
22	205705.0
23	324009.0
24	278127.0
25	254725.5
26	277206.0
27	314165.0
28	351124.0
29	394378.0
30	437632.0
31	472458.5
32	507285.0
33	560654.5
34	614024.0
35	682395.0
36	750766.0
37	822528.5
38	894291.0
39	957707.0
40	1021123.0
41	1089625.5
42	1217070.5
43	1276013.0
44	1336785.0
45	1397557.0
46	1433063.0
47	1468569.0
48	1493744.0
49	1518919.0
50	1514801.0
51	1510683.0
52	1462767.0
53	1414851.0
54	1348561.5
55	1282272.0
56	1194657.0
57	1107042.0
58	987733.0
59	762814.5
60	657205.0
61	572891.5
62	488578.0
63	414682.0
64	340786.0
65	275085.0
66	209384.0
67	164324.0
68	119264.0
69	87166.5
70	55069.0
71	39342.0
72	23615.0
73	17331.0
74	11047.0
75	7885.5
76	3388.0
77	2052.0
78	1516.0
79	980.0
80	754.0
81	528.0
82	382.5
83	237.0
84	185.5
85	134.0
86	88.5
87	43.0
88	37.0
89	31.0
90	25.5
91	20.0
92	15.0
93	9.5
94	9.0
95	7.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.053315E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.59729742584038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.77098630720889	33.4433399550293
2	14.773019720513725	13.767655875891668
3	5.220726973475321	7.298153026864111
4	2.4338333728251036	4.536402302338704
5	1.4021313986337247	3.2667766906122617
6	0.9067304350615399	2.5350712660574533
7	0.6054649749753843	1.9749122063899893
8	0.4479709018563485	1.6699386681537778
9	0.32124646556293607	1.347229539255251
>10	1.8962521048200127	16.320091763780674
>50	0.14839203415252716	4.732931385649505
>100	0.06821914974840598	5.562997651988474
>500	0.003188264019897347	0.9976484118751204
>1k	0.0017434932992486348	1.4142646257006548
>5k	6.29358978513174E-5	0.20139924092081296
>10k+	3.146794892565739E-5	0.9311873894922308
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	150230	0.7316461429444582	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	25908	0.12617645125078228	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.142876275681033E-4	1.9480693415282116E-5	0.0	0.0	0.0
2	0.002844181238631189	1.9480693415282116E-5	0.0	0.0	0.0
3	0.0028490514119850095	1.9480693415282116E-5	0.0	0.0	0.0
4	0.0032824968404750366	1.9480693415282116E-5	0.0	0.0	0.0
5	0.003287367013828857	1.9480693415282116E-5	0.0	0.0	4.870173353820529E-6
6	0.003287367013828857	1.9480693415282116E-5	0.0	0.0	9.740346707641058E-6
7	0.003287367013828857	1.9480693415282116E-5	0.0	0.0	9.740346707641058E-6
8	0.0032922371871826777	1.9480693415282116E-5	0.0	0.0	9.740346707641058E-6
9	0.0032971073605364983	2.4350866769102648E-5	0.0	4.870173353820529E-6	1.9480693415282116E-5
10	0.003301977533890319	2.9221040122923176E-5	0.0	4.870173353820529E-6	2.4350866769102648E-5
11	0.00331171788059796	2.9221040122923176E-5	0.0	4.870173353820529E-6	4.383156018438476E-5
12	0.0033214582273056008	2.9221040122923176E-5	0.0	4.870173353820529E-6	5.3571906892025824E-5
13	0.0033360687473670625	2.9221040122923176E-5	0.0	4.870173353820529E-6	5.844208024584635E-5
14	0.003340938920720883	2.9221040122923176E-5	0.0	4.870173353820529E-6	7.305260030730794E-5
15	0.003360419614136165	2.9221040122923176E-5	0.0	4.870173353820529E-6	7.792277366112846E-5
16	0.003370159960843806	2.9221040122923176E-5	0.0	4.870173353820529E-6	7.792277366112846E-5
17	0.0033799003075514473	2.9221040122923176E-5	0.0	9.740346707641058E-6	7.792277366112846E-5
18	0.003394510827612909	2.9221040122923176E-5	0.0	1.9480693415282116E-5	7.792277366112846E-5
19	0.0034091213476743704	2.9221040122923176E-5	0.0	2.4350866769102648E-5	1.0227364043023112E-4
20	0.003423731867735832	2.9221040122923176E-5	0.0	2.4350866769102648E-5	1.2175433384551323E-4
21	0.0034480827345049346	2.9221040122923176E-5	0.0	2.4350866769102648E-5	1.3636485390697482E-4
22	0.0034773037746278577	4.383156018438476E-5	0.0	2.4350866769102648E-5	1.3636485390697482E-4
23	0.00349678446804314	4.383156018438476E-5	0.0	2.9221040122923176E-5	1.3636485390697482E-4
24	0.0035016546413969606	4.383156018438476E-5	0.0	2.9221040122923176E-5	1.4123502726079535E-4
25	0.003526005508166063	4.8701733538205296E-5	0.0	2.9221040122923176E-5	1.4610520061461587E-4
26	0.0035600967216428067	4.8701733538205296E-5	0.0	2.9221040122923176E-5	1.5584554732225693E-4
27	0.003599058108473371	4.8701733538205296E-5	0.0	3.4091213476743704E-5	1.6071572067607746E-4
28	0.003662370362073038	4.8701733538205296E-5	0.0	3.4091213476743704E-5	1.8019641409135957E-4
29	0.0037159422689650636	4.8701733538205296E-5	0.0	3.896138683056423E-5	1.8019641409135957E-4
30	0.0037841246959185513	4.8701733538205296E-5	0.0	3.896138683056423E-5	1.8993676079900063E-4
31	0.0039058790297640646	5.3571906892025824E-5	0.0	3.896138683056423E-5	1.9480693415282118E-4
32	0.004022763190255757	5.3571906892025824E-5	0.0	4.383156018438476E-5	1.9480693415282118E-4
33	0.0041445175241012705	5.3571906892025824E-5	0.0	4.8701733538205296E-5	2.0454728086046224E-4
34	0.004251661337885322	5.3571906892025824E-5	0.0	5.3571906892025824E-5	2.0454728086046224E-4
35	0.004339324458254091	5.3571906892025824E-5	0.0	5.844208024584635E-5	2.142876275681033E-4
36	0.004480559485514887	5.3571906892025824E-5	0.0	6.331225359966688E-5	2.337683209833854E-4
37	0.004631534859483324	5.844208024584635E-5	0.0	6.331225359966688E-5	2.3863849433720594E-4
38	0.004797120753513222	5.844208024584635E-5	0.0	6.331225359966688E-5	2.3863849433720594E-4
39	0.005030889074496607	6.331225359966688E-5	4.870173353820529E-6	6.818242695348741E-5	2.532490143986675E-4
40	0.005293878435602915	6.331225359966688E-5	4.870173353820529E-6	6.818242695348741E-5	2.7272970781394963E-4
41	0.005610439703601249	6.331225359966688E-5	4.870173353820529E-6	6.818242695348741E-5	3.019507479368728E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	95210	0.0	41.461292	1
GTGGTAT	101905	0.0	39.46607	6
CAACGCA	102360	0.0	39.171257	13
TCAACGC	102450	0.0	39.15749	12
AGTGGTA	102565	0.0	39.12275	5
GGTATCA	102890	0.0	38.996887	8
GTATCAA	103125	0.0	38.91942	9
ACGCAGA	103545	0.0	38.82056	15
AACGCAG	103930	0.0	38.75589	14
TGGTATC	103715	0.0	38.596054	7
ATCAACG	104085	0.0	38.567223	11
TATCAAC	104435	0.0	38.435722	10
CGCAGAG	105145	0.0	38.225353	16
CAGTGGT	105190	0.0	38.121872	4
GCAGTGG	105880	0.0	38.059875	3
AGCAGTG	106290	0.0	38.014767	2
AGAGTAC	103915	0.0	37.911175	19
CAGAGTA	106685	0.0	37.07883	18
GCAGAGT	108385	0.0	36.62518	17
GTACATG	76245	0.0	36.249393	22
>>END_MODULE