FastQCFastQC Report
Thu 26 May 2016
SRR805242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22229782
Sequences flagged as poor quality0
Sequence length53
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2406921.082745660753668No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC555520.24989898686365886No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG355030.15970916853795508No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG237320.10675768210412499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC1858350.041.42201219
GTACATG1431850.040.4695322
ACATGGG1424650.040.33095624
AGTACAT1444850.040.2501621
GAGTACA1469000.040.0859720
TACATGG1443550.040.02105323
GAGTACT451700.039.75802620
CAGAGTA1968250.039.5509218
GTACTTT447300.039.47663522
CATGGGG920350.039.01814325
AGTACTT474650.037.508921
TACTTTT472650.037.3195823
ATGGGGG435400.036.6425126
ACTTTTT485300.036.35163524
TCAACGC2348900.034.65221812
ATCAACG2366900.034.45521511
CAACGCA2364950.034.4011513
ACGCAGA2405650.033.90900415
AACGCAG2409950.033.8972614
CGCAGAG2437750.033.43068316
Produced by FastQC (version 0.11.5)