##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805242_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22229782
Sequences flagged as poor quality	0
Sequence length	53
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.17291833990995	37.0	36.0	37.0	35.0	37.0
2	36.12376860015991	37.0	36.0	37.0	35.0	37.0
3	37.77793583400863	39.0	38.0	39.0	35.0	39.0
4	37.70083854173649	39.0	38.0	39.0	35.0	39.0
5	37.47495985340747	39.0	37.0	39.0	35.0	39.0
6	37.5789260101606	39.0	37.0	39.0	35.0	39.0
7	37.46661456239202	39.0	37.0	39.0	35.0	39.0
8	38.479895079492906	40.0	38.0	40.0	35.0	40.0
9	38.48846488912937	40.0	38.0	40.0	35.0	40.0
10	38.198719267692326	40.0	38.0	40.0	34.0	40.0
11	38.39023549578669	40.0	38.0	40.0	35.0	40.0
12	38.510170635051665	40.0	38.0	40.0	36.0	40.0
13	38.47366415918969	40.0	39.0	40.0	35.0	40.0
14	38.5543590575922	40.0	39.0	40.0	35.0	40.0
15	38.50907354826961	40.0	39.0	40.0	35.0	40.0
16	38.50632745746225	40.0	39.0	40.0	35.0	40.0
17	38.37767495875578	40.0	38.0	40.0	35.0	40.0
18	38.42710567292113	40.0	38.0	40.0	35.0	40.0
19	38.396638392585224	40.0	38.0	40.0	35.0	40.0
20	38.33076194809288	40.0	38.0	40.0	35.0	40.0
21	38.16791082341698	40.0	38.0	40.0	34.0	40.0
22	38.18815209253964	40.0	38.0	40.0	34.0	40.0
23	37.731918558625544	40.0	38.0	40.0	33.0	40.0
24	37.865953881149174	40.0	38.0	40.0	34.0	40.0
25	37.93764005422995	40.0	38.0	40.0	34.0	40.0
26	37.923641806293915	40.0	38.0	40.0	34.0	40.0
27	37.82647823536911	40.0	38.0	40.0	34.0	40.0
28	37.67576659096342	40.0	38.0	40.0	33.0	40.0
29	37.76127570661737	40.0	38.0	40.0	33.0	40.0
30	37.534513788754204	40.0	38.0	40.0	33.0	40.0
31	37.709683792670575	40.0	38.0	40.0	33.0	40.0
32	37.28804497497996	40.0	38.0	40.0	31.0	40.0
33	37.605895910270284	40.0	38.0	40.0	33.0	40.0
34	37.58337189271582	40.0	38.0	40.0	33.0	40.0
35	37.69458940263112	40.0	38.0	40.0	33.0	40.0
36	37.64963884935984	40.0	38.0	40.0	33.0	40.0
37	37.40975062193592	40.0	37.0	40.0	33.0	40.0
38	37.513882457326844	40.0	37.0	40.0	33.0	40.0
39	37.419212028260105	40.0	37.0	40.0	33.0	40.0
40	37.30923551117145	40.0	37.0	40.0	32.0	40.0
41	37.37706878996834	40.0	37.0	40.0	32.0	40.0
42	37.473267888996844	40.0	37.0	40.0	33.0	40.0
43	37.39007705968507	40.0	37.0	40.0	33.0	40.0
44	37.37865549018879	40.0	37.0	40.0	33.0	40.0
45	37.3401555624792	40.0	37.0	40.0	33.0	40.0
46	37.10547084987158	40.0	36.0	40.0	32.0	40.0
47	37.12745932461236	40.0	36.0	40.0	32.0	40.0
48	37.16226155524152	40.0	36.0	40.0	32.0	40.0
49	37.01900787870974	40.0	36.0	40.0	32.0	40.0
50	36.930540029587334	39.0	36.0	40.0	32.0	40.0
51	36.77740771366989	39.0	35.0	40.0	31.0	40.0
52	36.56180825345026	39.0	35.0	40.0	31.0	40.0
53	36.00418339685022	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	8.0
12	28.0
13	76.0
14	191.0
15	456.0
16	1058.0
17	1923.0
18	3333.0
19	5212.0
20	8793.0
21	14159.0
22	22900.0
23	35099.0
24	52115.0
25	74075.0
26	101963.0
27	135622.0
28	176273.0
29	223214.0
30	280346.0
31	346856.0
32	428149.0
33	533041.0
34	684355.0
35	922345.0
36	1339682.0
37	2221744.0
38	3981590.0
39	1.0635168E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.794928983109235	36.40723512268361	30.53688065856876	21.260955235638388
2	29.632854699159893	42.386947384369314	12.508341287377448	15.471856629093347
3	34.332127053697604	26.191174524338567	18.38603725398657	21.090661167977267
4	22.70709177444925	24.409807527577193	19.830500362081825	33.05260033589173
5	21.487817559344485	32.81800964130013	22.912599862652723	22.781572936702663
6	30.06703349587504	23.764479561697907	22.306246637956235	23.862240304470824
7	23.647258439151585	22.72913427581071	30.880325322128666	22.743281962909037
8	29.88832279146957	25.34126065653725	23.31783550553937	21.452581046453805
9	30.15572982227176	23.84444435847369	23.613196026843628	22.38662979241092
10	21.872985529052873	24.91356865307991	31.43545447274292	21.777991345124303
11	22.024021648075543	32.44857731848202	24.030352614344128	21.49704841909831
12	21.43699384906249	24.397576188556418	31.41530132864101	22.75012863374009
13	21.799093666325653	24.981756456271142	23.94543950093618	29.273710376467026
14	22.424286481981696	32.37989018515791	23.386122275063244	21.80970105779715
15	21.38812247461536	32.50651760777501	23.44366220055599	22.661697717053634
16	22.476954564826592	24.442250490805534	23.457877364699304	29.622917579668574
17	30.37339727398136	23.76776794302346	23.428277434299627	22.430557348695547
18	21.8580461112934	24.15931924118734	23.83174517860769	30.150889468911572
19	22.507040329950154	31.898347001333615	23.53243500093703	22.0621776677792
20	30.320927123801756	24.490878947890717	23.642593526108353	21.545600402199174
21	22.61360007938899	31.55994962073852	23.664060223352617	22.16239007651987
22	29.97375772735873	24.410594759768674	23.723057653017023	21.892589859855576
23	22.584562457697515	24.101585881498973	31.34059074443465	21.97326091636886
24	22.01755734716607	31.21676136994956	24.329140969533576	22.4365403133508
25	22.52935273949155	24.84863324345691	23.429289589974385	29.192724427077156
26	22.415716897268716	30.06127545470306	25.44333993018915	22.07966771783907
27	22.922491997447388	24.171860974615043	30.9599302413312	21.945716786606365
28	28.501593043062677	24.405156109942958	25.08648532855608	22.006765518438282
29	28.39853310302368	24.34897922075889	24.912700448434446	22.33978722778298
30	28.929626930214607	23.97947492242614	24.807386775092983	22.28351137226627
31	26.307419478967446	25.028396589764128	25.755268315271827	22.908915615996595
32	25.222613519106936	25.7999876022176	25.93603032184481	23.04136855683065
33	24.80480015503526	25.526215236838578	26.45110509855652	23.217879509569638
34	24.58232833772279	25.147070718012436	26.841248375715065	23.429352568549707
35	23.85477284482592	25.7111833125489	26.65911883436374	23.774925008261437
36	24.347778129358176	25.503637417586912	26.86782983296912	23.28075462008579
37	24.6240606408106	25.199972721279945	26.90330476475208	23.27266187315737
38	24.161393035703185	25.60126320626986	27.005384038403975	23.23195971962298
39	24.90778811955961	25.627376822678695	26.284670717868487	23.18016433989321
40	24.484495619435222	25.481509445301803	26.23839046194875	23.79560447331422
41	24.29545642867753	25.673342185721836	26.41037145573447	23.620829929866158
42	24.332267405951168	26.027245791254273	26.244530873042297	23.395955929752258
43	24.449371568286185	25.25915908667031	27.05010332534975	23.24136601969376
44	24.548814738714036	25.027195498363415	26.69774269491262	23.726247068009933
45	24.71321131264355	25.056039685859265	26.442472535268223	23.788276466228954
46	24.499907376509586	24.872290695428322	26.405490616147294	24.222311311914797
47	24.195347484739166	25.188074269014425	26.7470639163263	23.869514329920104
48	24.36099463323572	25.126800613699224	26.65374316311334	23.858461589951716
49	23.773004161714226	25.473929523915263	26.557750318919005	24.19531599545151
50	24.349181651893844	25.40384336652514	26.584642170579993	23.662332811001026
51	24.208190615634468	25.274530357517676	26.652807481422897	23.864471545424962
52	24.18388988250087	25.330360864537493	26.48221651476384	24.00353273819779
53	24.60863088985758	25.324607321835185	26.04702556237394	24.0197362259333
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2183.0
1	1409.0
2	635.0
3	551.5
4	468.0
5	487.5
6	507.0
7	609.5
8	914.5
9	1117.0
10	1723.0
11	2329.0
12	3561.0
13	4793.0
14	6281.5
15	7770.0
16	11638.5
17	15507.0
18	21995.5
19	28484.0
20	41858.5
21	55233.0
22	210686.0
23	366139.0
24	275165.5
25	192940.0
26	201688.0
27	225275.5
28	248863.0
29	280725.0
30	312587.0
31	343588.5
32	374590.0
33	422290.0
34	469990.0
35	532324.5
36	594659.0
37	669089.5
38	743520.0
39	825192.5
40	906865.0
41	1010357.0
42	1242998.0
43	1372147.0
44	1502168.5
45	1632190.0
46	1700763.5
47	1769337.0
48	1814990.5
49	1860644.0
50	1812797.0
51	1764950.0
52	1699261.0
53	1633572.0
54	1581343.0
55	1529114.0
56	1457250.5
57	1385387.0
58	1240830.0
59	965758.5
60	835244.0
61	735153.0
62	635062.0
63	545395.0
64	455728.0
65	372916.0
66	290104.0
67	230619.5
68	171135.0
69	130189.0
70	89243.0
71	64240.0
72	39237.0
73	28760.5
74	18284.0
75	13393.5
76	6023.5
77	3544.0
78	2565.0
79	1586.0
80	1271.0
81	956.0
82	720.0
83	484.0
84	339.5
85	195.0
86	134.0
87	73.0
88	66.0
89	59.0
90	44.5
91	30.0
92	22.0
93	9.5
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.2229782E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.72131590114938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.39235377689499	36.45543647019568
2	11.785760891984035	11.248640373235643
3	4.1282796132999495	5.910208066636851
4	2.1499039911185776	4.10384990069246
5	1.2599304524349897	3.0062769567064103
6	0.8256267433540743	2.3639996781622177
7	0.5859619421933674	1.95740124646225
8	0.4500294581049844	1.7180798348040822
9	0.3156505074915589	1.355693182412652
>10	1.891753276743497	17.23080243828354
>50	0.1561987913116506	5.048259112346683
>100	0.05148779785945997	4.4828722125945655
>500	0.004765423039421414	1.562786503675312
>1k	0.0021935186743597397	1.7103986677010579
>5k	5.662663364891324E-5	0.1953580919653955
>10k+	4.7188861374092946E-5	1.6499372641251637
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	240692	1.082745660753668	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	55552	0.24989898686365886	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	35503	0.15970916853795508	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	23732	0.10675768210412499	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4291736194264073E-4	0.0	0.0	0.0	0.0
2	0.016351937234472206	0.0	0.0	4.498469665604458E-6	8.996939331208915E-6
3	0.016414915809790667	0.0	0.0	4.498469665604458E-6	1.799387866241783E-5
4	0.01731460974291156	0.0	0.0	4.498469665604458E-6	1.799387866241783E-5
5	0.01732810515190837	0.0	0.0	4.498469665604458E-6	1.799387866241783E-5
6	0.01733260362157398	8.996939331208915E-6	0.0	4.498469665604458E-6	1.799387866241783E-5
7	0.017359594439567604	8.996939331208915E-6	0.0	4.498469665604458E-6	1.799387866241783E-5
8	0.01758901639251343	8.996939331208915E-6	0.0	8.996939331208915E-6	2.249234832802229E-5
9	0.018853086368548284	1.3495408996813375E-5	0.0	1.3495408996813375E-5	3.148928765923121E-5
10	0.022870219779933063	1.3495408996813375E-5	0.0	1.799387866241783E-5	4.498469665604458E-5
11	0.030872997315043397	1.3495408996813375E-5	0.0	2.249234832802229E-5	5.39816359872535E-5
12	0.04320330266846521	1.3495408996813375E-5	0.0	2.699081799362675E-5	6.297857531846242E-5
13	0.05682916728558112	1.3495408996813375E-5	0.0	2.699081799362675E-5	7.197551464967132E-5
14	0.07556529344282369	1.3495408996813375E-5	0.0	3.148928765923121E-5	7.197551464967132E-5
15	0.10005946077203996	1.3495408996813375E-5	0.0	3.598775732483566E-5	7.197551464967132E-5
16	0.12638900372482284	1.799387866241783E-5	0.0	3.598775732483566E-5	8.097245398088024E-5
17	0.1619539049010917	1.799387866241783E-5	0.0	3.598775732483566E-5	9.446786297769362E-5
18	0.20577799638341032	1.799387866241783E-5	0.0	4.498469665604458E-5	9.446786297769362E-5
19	0.24592233967926452	1.799387866241783E-5	0.0	4.498469665604458E-5	1.1246174164011144E-4
20	0.3024905957242406	1.799387866241783E-5	0.0	4.498469665604458E-5	1.3045562030252928E-4
21	0.3629635234389613	2.249234832802229E-5	0.0	4.498469665604458E-5	1.3045562030252928E-4
22	0.4178853395863261	2.249234832802229E-5	0.0	4.948316632164904E-5	1.3045562030252928E-4
23	0.4903916736565388	2.699081799362675E-5	0.0	4.948316632164904E-5	1.6194490796176048E-4
24	0.5566226425432332	2.699081799362675E-5	0.0	4.948316632164904E-5	1.6194490796176048E-4
25	0.6364839745167091	2.699081799362675E-5	0.0	5.39816359872535E-5	1.6644337762736496E-4
26	0.7290084985988616	2.699081799362675E-5	0.0	5.39816359872535E-5	1.6644337762736496E-4
27	0.8066790758451883	2.699081799362675E-5	0.0	6.297857531846242E-5	1.8893572595538724E-4
28	0.9045027971934227	2.699081799362675E-5	0.0	6.297857531846242E-5	2.15926543949014E-4
29	1.000999469990304	2.699081799362675E-5	0.0	6.297857531846242E-5	2.15926543949014E-4
30	1.081292655051678	2.699081799362675E-5	0.0	6.297857531846242E-5	2.249234832802229E-4
31	1.168499987989086	2.699081799362675E-5	0.0	6.297857531846242E-5	2.249234832802229E-4
32	1.2586043353911434	2.699081799362675E-5	0.0	6.747704498406686E-5	2.339204226114318E-4
33	1.3445880845795068	3.148928765923121E-5	0.0	7.197551464967132E-5	2.4291736194264073E-4
34	1.4474815812408777	3.148928765923121E-5	0.0	7.197551464967132E-5	2.4291736194264073E-4
35	1.537154075555037	3.148928765923121E-5	0.0	7.647398431527578E-5	2.5191430127384967E-4
36	1.6209065837892607	3.148928765923121E-5	0.0	8.097245398088024E-5	2.789051192674764E-4
37	1.7146771839687858	3.148928765923121E-5	0.0	8.097245398088024E-5	2.8340358893308085E-4
38	1.7934858740405102	3.148928765923121E-5	0.0	8.54709236464847E-5	2.8340358893308085E-4
39	1.8814039651850838	3.148928765923121E-5	0.0	8.54709236464847E-5	2.9689899792989424E-4
40	1.9673157388587976	3.148928765923121E-5	0.0	8.54709236464847E-5	2.9689899792989424E-4
41	2.0422512465484366	3.148928765923121E-5	0.0	8.996939331208916E-5	3.0589593726110313E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGTAC	185835	0.0	41.422012	19
GTACATG	143185	0.0	40.46953	22
ACATGGG	142465	0.0	40.330956	24
AGTACAT	144485	0.0	40.25016	21
GAGTACA	146900	0.0	40.08597	20
TACATGG	144355	0.0	40.021053	23
GAGTACT	45170	0.0	39.758026	20
CAGAGTA	196825	0.0	39.55092	18
GTACTTT	44730	0.0	39.476635	22
CATGGGG	92035	0.0	39.018143	25
AGTACTT	47465	0.0	37.5089	21
TACTTTT	47265	0.0	37.31958	23
ATGGGGG	43540	0.0	36.64251	26
ACTTTTT	48530	0.0	36.351635	24
TCAACGC	234890	0.0	34.652218	12
ATCAACG	236690	0.0	34.455215	11
CAACGCA	236495	0.0	34.40115	13
ACGCAGA	240565	0.0	33.909004	15
AACGCAG	240995	0.0	33.89726	14
CGCAGAG	243775	0.0	33.430683	16
>>END_MODULE