FastQCFastQC Report
Thu 26 May 2016
SRR805241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21578159
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT150050.019.4984991
CAACGCA273100.014.93811712
TCAACGC274800.014.88846411
AGTGGTA282750.014.7856754
AGCAGTG287950.014.6655661
ACGCAGA282950.014.56759114
GTGGTAT287750.014.4225895
GGTATCA286050.014.3768577
AACGCAG285600.014.35836913
GTATCAA290050.014.28391558
GCAGTGG297600.014.0636752
ATCAACG299150.013.88082910
TGGTATC300700.013.7389416
TATCAAC304400.013.5873849
CGCAGAG303950.013.53791815
AGAGTAC303700.013.41751818
CAGTGGT318450.013.2166733
CAGAGTA329950.012.37141917
ACATGGG267850.012.09874823
GCAGAGT346000.011.87225316
Produced by FastQC (version 0.11.5)