##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805241_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21578159
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.117059940099615	37.0	36.0	37.0	35.0	37.0
2	36.10015530055182	37.0	36.0	37.0	35.0	37.0
3	37.80591068960054	39.0	38.0	39.0	35.0	39.0
4	37.78299900376116	39.0	38.0	39.0	35.0	39.0
5	37.579297288522156	39.0	38.0	39.0	35.0	39.0
6	37.64222137764394	39.0	38.0	39.0	35.0	39.0
7	37.656931529700934	39.0	38.0	39.0	35.0	39.0
8	38.60662663575702	40.0	39.0	40.0	36.0	40.0
9	38.56685799747791	40.0	39.0	40.0	36.0	40.0
10	38.57102420090611	40.0	39.0	40.0	36.0	40.0
11	38.5773013351139	40.0	39.0	40.0	36.0	40.0
12	38.596408942950134	40.0	39.0	40.0	36.0	40.0
13	38.52835823482439	40.0	39.0	40.0	36.0	40.0
14	38.5723869677668	40.0	39.0	40.0	36.0	40.0
15	38.54475481434723	40.0	39.0	40.0	35.0	40.0
16	38.56059569308021	40.0	39.0	40.0	36.0	40.0
17	38.4458876681741	40.0	39.0	40.0	35.0	40.0
18	38.46948365706268	40.0	39.0	40.0	35.0	40.0
19	38.43943911063034	40.0	39.0	40.0	35.0	40.0
20	38.35983852932032	40.0	38.0	40.0	35.0	40.0
21	38.289957312855094	40.0	38.0	40.0	35.0	40.0
22	38.237426418073944	40.0	38.0	40.0	34.0	40.0
23	38.16463930032214	40.0	38.0	40.0	34.0	40.0
24	38.10230492786711	40.0	38.0	40.0	34.0	40.0
25	38.07609532398014	40.0	38.0	40.0	34.0	40.0
26	38.0217420309119	40.0	38.0	40.0	34.0	40.0
27	37.91091417947194	40.0	38.0	40.0	34.0	40.0
28	37.740957836115676	40.0	38.0	40.0	33.0	40.0
29	37.83613222054764	40.0	38.0	40.0	33.0	40.0
30	37.598173412291565	40.0	38.0	40.0	33.0	40.0
31	37.82983617833199	40.0	38.0	40.0	33.0	40.0
32	37.43000735141492	40.0	38.0	40.0	32.0	40.0
33	37.789587378608154	40.0	38.0	40.0	33.0	40.0
34	37.78708067727187	40.0	38.0	40.0	33.0	40.0
35	37.89784133113488	40.0	38.0	40.0	33.0	40.0
36	37.85008197409241	40.0	38.0	40.0	33.0	40.0
37	37.62591396235425	40.0	38.0	40.0	33.0	40.0
38	37.776971056705996	40.0	38.0	40.0	33.0	40.0
39	37.700521300264775	40.0	38.0	40.0	33.0	40.0
40	37.60044371718644	40.0	38.0	40.0	33.0	40.0
41	37.6731247091098	40.0	38.0	40.0	33.0	40.0
42	37.74511055368533	40.0	38.0	40.0	33.0	40.0
43	37.665437769737444	40.0	38.0	40.0	33.0	40.0
44	37.67986096496925	40.0	38.0	40.0	33.0	40.0
45	37.63607298472497	40.0	37.0	40.0	33.0	40.0
46	37.38152318740445	40.0	37.0	40.0	33.0	40.0
47	37.42769686700334	40.0	37.0	40.0	33.0	40.0
48	37.47188905225882	40.0	37.0	40.0	33.0	40.0
49	37.35497411989596	40.0	37.0	40.0	33.0	40.0
50	37.2887334364345	40.0	37.0	40.0	32.0	40.0
51	37.188387155734645	40.0	36.0	40.0	32.0	40.0
52	37.06092827474299	39.0	36.0	40.0	32.0	40.0
53	36.545779785940034	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	5.0
12	17.0
13	69.0
14	163.0
15	465.0
16	974.0
17	1828.0
18	3030.0
19	4946.0
20	7938.0
21	13014.0
22	20404.0
23	30573.0
24	45707.0
25	64624.0
26	89347.0
27	118517.0
28	154844.0
29	197492.0
30	249116.0
31	308485.0
32	382868.0
33	472564.0
34	601198.0
35	801096.0
36	1149582.0
37	1857930.0
38	3771882.0
39	1.1229476E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.007798348320632	26.150099273992744	29.2228544613097	30.619247916376924
2	25.819844037667906	24.42308447166415	29.80974882982371	19.947322660844236
3	16.318685945357988	25.144846694289352	32.86834154850745	25.66812581184521
4	17.848394758792907	28.428871063560152	31.44293727745727	22.279796900189677
5	24.17597812677161	27.44850012459358	28.77884531298523	19.596676435649584
6	22.346799835889612	26.55772904444721	29.6064414021604	21.489029717502778
7	23.989687906183285	27.156742148391803	28.271100421495643	20.582469523929266
8	21.665884471423162	25.642321942293595	29.012257255125423	23.679536331157816
9	21.464472478861612	24.90147560781251	30.73567582850789	22.898376084817986
10	22.575475507433236	26.381064297468566	27.848891093999264	23.19456910109894
11	22.437878041402882	26.786274955152567	27.831688514298186	22.944158489146364
12	22.52753814632657	26.582309454666635	27.604018489251096	23.286133909755694
13	22.936409913375837	26.894083040170386	26.775120157377653	23.394386889076124
14	23.176064278699588	27.101473299923317	26.691688572690563	23.030773848686536
15	22.884385085863908	26.76247310996272	26.756610700662648	23.59653110351073
16	23.410083316190228	26.622688246944513	26.48478955039677	23.482438886468486
17	24.18019535401514	26.191984218857595	26.301868477287616	23.325951949839652
18	23.149838686423617	26.64048865336473	26.477346839459287	23.732325820752365
19	23.826754636482196	26.53314863422779	26.301840671393702	23.33825605789632
20	23.7566142690857	26.47444575786099	26.421966767415146	23.346973205638164
21	23.649682069726154	26.628207716886322	26.521196734160686	23.20091347922684
22	23.617723828988378	26.15682366600413	26.70901627891425	23.516436226093244
23	23.530677478092546	26.588973600574544	26.807940380826743	23.072408540506167
24	23.277741164109507	26.49038316938901	26.59374694569634	23.638128720805142
25	23.38331550898295	26.3746967477624	26.44553226250673	23.796455480747916
26	23.6306628382894	26.21016463916129	26.97837197325314	23.180800549296166
27	23.867684912322687	25.962367781236573	26.88805379550684	23.281893510933905
28	23.902002019727448	26.000202334221377	26.53674949748957	23.56104614856161
29	24.032708258382932	26.091113704371168	26.579028359184857	23.297149678061043
30	24.31914140590029	25.924588840039597	26.371193205129316	23.385076548930797
31	23.66279718302196	26.148560681196205	26.42989144718046	23.758750688601378
32	24.235218583754065	26.15034489272231	26.318510304794767	23.295926218728855
33	23.58064930377054	26.184078076354893	26.473968423348815	23.761304196525757
34	23.710915282439064	26.183526592792276	26.32770942136445	23.777848703404214
35	23.807902240408925	26.233558664573746	26.380118897075512	23.578420197941817
36	23.650233553288768	26.176297060374797	26.434405270625728	23.739064115710708
37	23.999267036636443	26.14096040352655	26.12108845800979	23.738684101827225
38	23.922689604799	26.305450803286785	26.2962238808232	23.475635711091016
39	23.682636688329158	26.191201019512366	26.37033585673365	23.755826435424822
40	24.062302998138072	26.205437637196017	26.115754360694073	23.61650500397184
41	23.870947470541857	26.24716964964435	26.399416187451397	23.4824666923624
42	23.66119370980629	26.154135762925833	26.472244457926188	23.71242606934169
43	23.893080962096906	26.11188470712446	26.23445772181028	23.760576608968357
44	23.916822561183277	26.118604464820194	26.306363763470276	23.658209210526255
45	23.73169555382366	26.03635463062442	26.468388707303532	23.76356110824839
46	23.95602887160114	26.150785152709272	26.144881034568336	23.74830494112125
47	23.876156441334963	26.166986720229467	26.304366373424166	23.652490465011404
48	23.65612376848275	26.083379031547594	26.39674682163571	23.863750378333943
49	23.88223666347069	26.127650648973344	26.153797457883222	23.836315229672746
50	23.959045811090743	26.22281632089188	26.27553166143599	23.542606206581386
51	23.79696062115401	26.076654639536205	26.33917471828806	23.787210021021718
52	23.870632337077506	26.134222108568206	26.08066795689104	23.91447759746325
53	23.867684912322687	26.172821323635624	26.209548275179547	23.74994548886214
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4792.0
1	3411.0
2	2030.0
3	1689.0
4	1348.0
5	1434.5
6	1521.0
7	1744.5
8	2473.5
9	2979.0
10	4196.0
11	5413.0
12	8039.0
13	10665.0
14	13732.5
15	16800.0
16	24161.0
17	31522.0
18	44257.5
19	56993.0
20	77254.0
21	97515.0
22	135904.5
23	174294.0
24	194676.0
25	252709.5
26	290361.0
27	333124.0
28	375887.0
29	422531.0
30	469175.0
31	511132.5
32	553090.0
33	608758.5
34	664427.0
35	736755.5
36	809084.0
37	886401.0
38	963718.0
39	1029785.0
40	1095852.0
41	1165521.5
42	1299682.0
43	1364173.0
44	1414509.0
45	1464845.0
46	1499621.5
47	1534398.0
48	1541746.0
49	1549094.0
50	1548095.0
51	1547096.0
52	1499353.0
53	1451610.0
54	1395251.5
55	1338893.0
56	1244862.0
57	1150831.0
58	1040494.0
59	821679.0
60	713201.0
61	627949.5
62	542698.0
63	466171.0
64	389644.0
65	316392.5
66	243141.0
67	193126.5
68	143112.0
69	107328.5
70	71545.0
71	53499.0
72	35453.0
73	25165.5
74	14878.0
75	10941.5
76	5136.5
77	3268.0
78	2404.5
79	1541.0
80	1264.0
81	987.0
82	745.5
83	504.0
84	346.5
85	189.0
86	142.0
87	95.0
88	73.0
89	51.0
90	46.5
91	42.0
92	26.5
93	9.0
94	7.0
95	5.0
96	3.0
97	3.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.1578159E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.077517057907976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19923787273677	36.90591879440918
2	13.013276136641952	12.773185631506243
3	4.455949871018172	6.560608674122315
4	2.118616341230102	4.159057185035317
5	1.203086049633724	2.9522238061515105
6	0.7657750482300526	2.254940279521843
7	0.5398771983068442	1.8547082686355798
8	0.3679934081888735	1.4448162214069704
9	0.2889338249607873	1.276213925080767
>10	1.8282135520733847	17.41633651005326
>50	0.1592161298978377	5.302390305822284
>100	0.05435279155818458	4.377166510930968
>500	0.0034414569678737376	1.1942684505731465
>1k	0.002011386433632454	1.4444962417389708
>5k	1.8932121841379462E-5	0.08366919501177297
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.853726260891858E-5	4.634315652229645E-6	0.0	0.0	4.634315652229645E-6
2	3.244020956560752E-5	4.634315652229645E-6	0.0	4.634315652229645E-6	4.634315652229645E-6
3	4.634315652229646E-5	4.634315652229645E-6	0.0	4.634315652229645E-6	4.634315652229645E-6
4	4.634315652229646E-5	4.634315652229645E-6	0.0	4.634315652229645E-6	1.3902946956688938E-5
5	2.0390988869810442E-4	4.634315652229645E-6	0.0	1.3902946956688938E-5	1.853726260891858E-5
6	2.0390988869810442E-4	9.26863130445929E-6	0.0	1.853726260891858E-5	1.853726260891858E-5
7	2.0854420435033406E-4	9.26863130445929E-6	0.0	1.853726260891858E-5	2.317157826114823E-5
8	2.131785200025637E-4	9.26863130445929E-6	0.0	1.853726260891858E-5	3.244020956560752E-5
9	2.131785200025637E-4	1.3902946956688938E-5	4.634315652229645E-6	1.853726260891858E-5	3.707452521783716E-5
10	2.2708146695925264E-4	1.3902946956688938E-5	4.634315652229645E-6	1.853726260891858E-5	3.707452521783716E-5
11	2.5952167652486014E-4	1.3902946956688938E-5	4.634315652229645E-6	1.853726260891858E-5	3.707452521783716E-5
12	2.87327570438238E-4	1.3902946956688938E-5	4.634315652229645E-6	3.244020956560752E-5	4.634315652229646E-5
13	3.429393582649938E-4	1.3902946956688938E-5	4.634315652229645E-6	3.244020956560752E-5	6.488041913121503E-5
14	3.939168304395199E-4	1.3902946956688938E-5	4.634315652229645E-6	3.244020956560752E-5	6.951473478344469E-5
15	4.3562567130958667E-4	1.3902946956688938E-5	4.634315652229645E-6	3.707452521783716E-5	7.414905043567432E-5
16	4.727001965274239E-4	1.3902946956688938E-5	4.634315652229645E-6	3.707452521783716E-5	7.878336608790398E-5
17	5.329463000064092E-4	1.3902946956688938E-5	4.634315652229645E-6	3.707452521783716E-5	8.805199739236327E-5
18	5.561178782675575E-4	1.3902946956688938E-5	4.634315652229645E-6	3.707452521783716E-5	9.268631304459292E-5
19	5.74655140876476E-4	1.853726260891858E-5	4.634315652229645E-6	4.634315652229646E-5	1.0658926000128185E-4
20	5.978267191376243E-4	1.853726260891858E-5	9.26863130445929E-6	5.561178782675575E-5	1.2049220695797079E-4
21	6.395355600076911E-4	1.853726260891858E-5	9.26863130445929E-6	6.488041913121503E-5	1.2512652261020043E-4
22	6.905130321822172E-4	1.853726260891858E-5	9.26863130445929E-6	6.488041913121503E-5	1.3902946956688937E-4
23	7.229532417478247E-4	1.853726260891858E-5	9.26863130445929E-6	6.488041913121503E-5	1.5756673217580795E-4
24	7.46124820008973E-4	1.853726260891858E-5	9.26863130445929E-6	6.951473478344469E-5	1.6683536348026726E-4
25	7.692963982701212E-4	1.853726260891858E-5	9.26863130445929E-6	7.414905043567432E-5	1.7610399478472654E-4
26	8.11005239140188E-4	1.853726260891858E-5	1.3902946956688938E-5	9.268631304459292E-5	1.7610399478472654E-4
27	8.758856582714031E-4	1.853726260891858E-5	2.317157826114823E-5	9.732062869682256E-5	1.8537262608918584E-4
28	8.944229208803216E-4	1.853726260891858E-5	2.317157826114823E-5	9.732062869682256E-5	1.9000694174141548E-4
29	9.82474918272685E-4	1.853726260891858E-5	2.317157826114823E-5	1.0195494434905221E-4	1.9000694174141548E-4
30	0.0010427210217516703	1.853726260891858E-5	2.317157826114823E-5	1.0658926000128185E-4	1.9000694174141548E-4
31	0.0011354073347962631	2.317157826114823E-5	2.317157826114823E-5	1.1585789130574114E-4	1.9927557304587476E-4
32	0.0012327279634930857	2.7805893913377875E-5	2.317157826114823E-5	1.1585789130574114E-4	2.2708146695925264E-4
33	0.0013161456452332194	2.7805893913377875E-5	2.317157826114823E-5	1.2049220695797079E-4	2.5025304522040086E-4
34	0.0013810260643644344	2.7805893913377875E-5	2.317157826114823E-5	1.2049220695797079E-4	2.5025304522040086E-4
35	0.0014320035365389604	2.7805893913377875E-5	2.317157826114823E-5	1.2049220695797079E-4	3.0123051739492697E-4
36	0.001608107531323687	2.7805893913377875E-5	2.317157826114823E-5	1.2049220695797079E-4	3.0123051739492697E-4
37	0.0017749428948039543	2.7805893913377875E-5	2.317157826114823E-5	1.2976083826243007E-4	3.0123051739492697E-4
38	0.0019510468895886808	2.7805893913377875E-5	2.317157826114823E-5	1.2976083826243007E-4	3.0123051739492697E-4
39	0.0021781283565479336	2.7805893913377875E-5	2.317157826114823E-5	1.2976083826243007E-4	3.0123051739492697E-4
40	0.002368135298289349	2.7805893913377875E-5	2.317157826114823E-5	1.3439515391465973E-4	3.0586483304715663E-4
41	0.0025535079243785347	2.7805893913377875E-5	2.317157826114823E-5	1.43663785219119E-4	3.1049914869938624E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	15005	0.0	19.498499	1
CAACGCA	27310	0.0	14.938117	12
TCAACGC	27480	0.0	14.888464	11
AGTGGTA	28275	0.0	14.785675	4
AGCAGTG	28795	0.0	14.665566	1
ACGCAGA	28295	0.0	14.567591	14
GTGGTAT	28775	0.0	14.422589	5
GGTATCA	28605	0.0	14.376857	7
AACGCAG	28560	0.0	14.358369	13
GTATCAA	29005	0.0	14.2839155	8
GCAGTGG	29760	0.0	14.063675	2
ATCAACG	29915	0.0	13.880829	10
TGGTATC	30070	0.0	13.738941	6
TATCAAC	30440	0.0	13.587384	9
CGCAGAG	30395	0.0	13.537918	15
AGAGTAC	30370	0.0	13.417518	18
CAGTGGT	31845	0.0	13.216673	3
CAGAGTA	32995	0.0	12.371419	17
ACATGGG	26785	0.0	12.098748	23
GCAGAGT	34600	0.0	11.872253	16
>>END_MODULE