FastQCFastQC Report
Thu 26 May 2016
SRR805240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14755036
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT5823603.9468558395926654No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG1343990.9108686688395744No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT1032320.6996390927138368No Hit
TGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG1028570.6970975875626464No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA980140.6642748957034059No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC979110.6635768289552123No Hit
TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC958250.6494392829675237No Hit
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG939140.6364877727170575No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA912500.6184329201230008No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA896820.6078060399174898No Hit
AGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG856940.5807779798029635No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT850880.5766709074786398No Hit
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC835500.5662473476852242No Hit
TAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG828950.5618081853544783No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT828940.5618014080074085No Hit
TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG811970.5503002500298881No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA793520.5377960446860313No Hit
TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT742210.5030214768706766No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC735950.49877885760495605No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT726360.4922793817649784No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCG705710.4782841600657566No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC704410.47740310494667715No Hit
TCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT686740.4654275326742679No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC659140.4467220547615065No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC618630.41926702178158015No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA555450.3764477429943241No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT549930.3727066474117718No Hit
ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG531300.36008044982065784No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG524600.3555396272838643No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT499790.3387250292035885No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA489210.33155459600369663No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC474850.3218223256114048No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGT414530.28094136808612324No Hit
CTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC383930.2602026860524095No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC364300.2468987537543114No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG336050.2277527482820103No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA327220.2217683508193406No Hit
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT322730.21872532198498196No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA320910.21749184481827086No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA305260.20688529665396954No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACT305060.2067497497125727No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA303400.2056247100989791No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT297810.2018361730869379No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC286260.19400833722127145No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT241060.16337472846558965No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG221890.15038255413270424No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA214240.1451978836242758No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAATAAGCAGT209950.14229040173131396No Hit
AAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGT208850.14154489355363145No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGAAAGCAGT152000.10301567546158479No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG145450.034.70471222
AGTACAT148600.034.22207321
ACATGGG138350.034.1416724
TACATGG141200.033.7687723
CATGGGG85550.033.23787325
GAGTACA158150.033.01738720
CCCCGAT10500.031.55714244
ATGGGGG36000.030.87638926
CCGATCC10450.030.80861346
CGATCCC10800.030.46296347
ATGGGGC17750.030.31831226
TAATTGC11100.030.06306534
AATTGCA11800.028.67796735
GCAATCC12000.028.59166539
ATTGCAA11700.028.5213736
CCCGATC11700.028.5213745
TGGGGGG21700.028.37327427
TCCCCGA11700.028.32051543
ATGGGGA34050.027.88252626
ATAATTG12050.027.69294533
Produced by FastQC (version 0.11.5)