FastQCFastQC Report
Thu 26 May 2016
SRR805239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14225462
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCG2819401.9819391454562247No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT2336821.6427023600358286No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2232881.5696361917806254No Hit
CTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC1844651.2967241415428195No Hit
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG1728311.214941208939295No Hit
AGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG1674701.1772552624301411No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA1634381.1489117190007607No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC1570131.1037462263088538No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1548111.0882669399419154No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA1410200.9913210551615126No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1382100.9715677424044296No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG1320300.9281245136361829No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT1288230.9055804303579033No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT1265970.889932432422933No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1152440.8101248310951166No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC1122270.7889163810637574No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACT1060210.7452903814301427No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC1035140.7276670522194639No Hit
ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG981290.6898123941422781No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT956010.6720414423095714No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG900290.6328722399314693No Hit
TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG892920.6276913888631526No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA858940.6038046426892849No Hit
TGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG773020.5434059013338196No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA716250.5034985858455775No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT711530.5001805916742809No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA678050.4766453279338133No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC599180.4212024888892888No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC561940.39502407724965277No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA549440.3862370164146514No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA541300.3805148823988985No Hit
TCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT502720.3533944978377504No Hit
TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT464670.32664668465600627No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC460690.3238488844861418No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT449220.3157858774639446No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA412740.2901417191230766No Hit
TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC367280.2581849362783437No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT352200.2475842260869981No Hit
TAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG296440.20838690511422406No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG210980.1483115275974868No Hit
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC202820.14257533428439795No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC174520.12268142855395488No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA159430.11207368871394124No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG157360.11061855143966502No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCG152980.10753956532308054No Hit
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT152010.10685768940228443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA15150.020.78547927
GCGTTGC27450.018.40619323
CGTTGCT30100.016.9418624
ACATGGG90000.016.86777724
CGCAGCG21450.016.7622424
TTGCTAC31000.016.75322526
GTACATG94600.016.74312822
AGTACAT96250.016.60259821
GCGTAAG19000.016.57368328
CAGCGTA20150.016.44416826
GAGTACA99150.016.42511420
GTTGCTA32150.016.37325125
TACATGG93250.016.2042923
CGTAAGC19550.015.98721229
GCAGCGT22400.015.94642925
CATGGGG56900.015.94200425
TGCTACC34650.015.80230927
GCTACCA36400.015.17170328
CGAGTAA9800.015.10714329
CTACCAC36600.014.63934429
Produced by FastQC (version 0.11.5)