FastQCFastQC Report
Thu 26 May 2016
SRR805238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18511017
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT763680.4125543183283771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT567550.040.5199551
AGTGGTA678850.033.7103925
CAACGCA686600.033.53524413
GTGGTAT680800.033.527546
TCAACGC687050.033.482512
GGTATCA689050.033.3102848
GTATCAA690900.033.2891169
ACGCAGA694050.033.1549615
TGGTATC690550.033.0711757
AACGCAG695950.033.06444514
ATCAACG703700.032.82719811
TATCAAC702650.032.789310
AGCAGTG709500.032.5754052
CAGTGGT709850.032.42034
CGCAGAG711100.032.4029716
AGAGTAC701750.032.3926619
GCAGTGG719050.032.1100773
CAGAGTA726250.031.33225618
GCAGAGT743100.030.80520817
GTACATG568100.030.15164422
Produced by FastQC (version 0.11.5)