##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805238_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18511017
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.99092961775142	37.0	37.0	37.0	35.0	37.0
2	36.13629872415978	37.0	37.0	37.0	35.0	37.0
3	37.8527362381008	39.0	38.0	39.0	35.0	39.0
4	37.902789727868544	39.0	38.0	39.0	35.0	39.0
5	37.903907386611984	39.0	38.0	39.0	35.0	39.0
6	37.804778689361044	39.0	38.0	39.0	35.0	39.0
7	37.69267172084602	39.0	38.0	39.0	35.0	39.0
8	38.63711394138961	40.0	39.0	40.0	36.0	40.0
9	38.66960594331473	40.0	39.0	40.0	36.0	40.0
10	38.62015366308615	40.0	39.0	40.0	36.0	40.0
11	38.639410519692134	40.0	39.0	40.0	36.0	40.0
12	38.65674706041273	40.0	39.0	40.0	36.0	40.0
13	38.73555591246013	40.0	39.0	40.0	36.0	40.0
14	38.7459475079084	40.0	39.0	40.0	36.0	40.0
15	38.72126345084119	40.0	39.0	40.0	36.0	40.0
16	38.68148319457543	40.0	39.0	40.0	36.0	40.0
17	38.600721559490765	40.0	39.0	40.0	36.0	40.0
18	38.36859606363065	40.0	39.0	40.0	35.0	40.0
19	38.55278399884782	40.0	39.0	40.0	35.0	40.0
20	38.504521442554996	40.0	39.0	40.0	35.0	40.0
21	38.50618040056902	40.0	39.0	40.0	36.0	40.0
22	38.4951387597991	40.0	39.0	40.0	35.0	40.0
23	38.29314267282019	40.0	38.0	40.0	35.0	40.0
24	38.20211601555982	40.0	38.0	40.0	34.0	40.0
25	38.18933184492241	40.0	38.0	40.0	34.0	40.0
26	38.15482271989702	40.0	38.0	40.0	34.0	40.0
27	37.75696953873469	40.0	38.0	40.0	33.0	40.0
28	37.91748908231244	40.0	38.0	40.0	34.0	40.0
29	37.94407789696266	40.0	38.0	40.0	34.0	40.0
30	37.9457859608686	40.0	38.0	40.0	34.0	40.0
31	37.81710853595997	40.0	38.0	40.0	33.0	40.0
32	37.76683047722337	40.0	38.0	40.0	33.0	40.0
33	37.729350526770084	40.0	38.0	40.0	33.0	40.0
34	37.76489752021728	40.0	38.0	40.0	33.0	40.0
35	37.814338779981675	40.0	38.0	40.0	33.0	40.0
36	37.772049423324496	40.0	38.0	40.0	33.0	40.0
37	37.7219133340972	40.0	38.0	40.0	33.0	40.0
38	37.671242158115895	40.0	38.0	40.0	33.0	40.0
39	37.66129294786991	40.0	38.0	40.0	33.0	40.0
40	37.52127487106732	40.0	37.0	40.0	33.0	40.0
41	37.560867347266765	40.0	37.0	40.0	33.0	40.0
42	37.26606204294448	40.0	37.0	40.0	32.0	40.0
43	37.40874907089113	40.0	37.0	40.0	33.0	40.0
44	37.399209184454854	40.0	37.0	40.0	33.0	40.0
45	37.02056197128445	40.0	37.0	40.0	31.0	40.0
46	37.19605432807933	40.0	37.0	40.0	32.0	40.0
47	37.07696411277673	40.0	36.0	40.0	32.0	40.0
48	37.139549977183854	40.0	36.0	40.0	32.0	40.0
49	37.05709232507323	40.0	36.0	40.0	32.0	40.0
50	36.96806647630435	39.0	36.0	40.0	31.0	40.0
51	36.72430585526446	39.0	35.0	40.0	31.0	40.0
52	36.534659008740576	39.0	35.0	40.0	31.0	40.0
53	35.83832373985719	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	15.0
13	45.0
14	187.0
15	536.0
16	1284.0
17	2165.0
18	3437.0
19	5316.0
20	7863.0
21	12239.0
22	18773.0
23	28103.0
24	40117.0
25	56599.0
26	77432.0
27	102090.0
28	131250.0
29	166234.0
30	208112.0
31	256470.0
32	318210.0
33	396269.0
34	511315.0
35	694660.0
36	1023905.0
37	1696791.0
38	3419563.0
39	9332032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.612357386955022	38.54350628061116	15.634170721144061	19.209965611289753
2	30.905535876283835	29.704294475014525	25.165316416704712	14.224853231996923
3	25.85982715050178	24.231553566181695	25.766742043400427	24.1418772399161
4	22.403863601875575	30.314784973726727	23.287758851931258	23.99359257246644
5	22.871914600910365	30.2713081620529	22.812344670203696	24.044432566833038
6	25.01017637226523	24.704752850694263	27.89983392052419	22.38523685651631
7	22.5429267338472	25.98496884314892	27.08977578055274	24.382328642451142
8	24.790161448179752	24.88315471807951	26.33929837566461	23.98738545807613
9	25.39880439848335	25.516880028795825	26.0976152741905	22.98670029853033
10	22.484345403604785	26.85111250235468	28.368371116508616	22.296170977531922
11	22.646989087633596	28.791173386097586	26.36101517274821	22.200822353520607
12	22.80116214036214	25.344301720429513	28.654082052866137	23.200454086342205
13	22.732343663235792	26.33641360709679	25.641821840474783	25.289420889192638
14	22.751191898316552	28.74207289637301	25.694585013886595	22.81215019142384
15	22.872184710326827	27.919108928482967	25.538240281449692	23.670466079740514
16	23.726616425234766	25.609613993655778	25.109328136860338	25.55444144424912
17	25.94862832225804	25.369864875603536	25.31796065013608	23.363546152002346
18	23.22723273389031	25.755748590150397	25.05610037525221	25.960918300707085
19	23.937047867224152	28.129432326705768	24.886428444207038	23.047091361863046
20	25.94491161668751	25.964824082869136	25.191716911069772	22.898547389373583
21	23.86865076078748	27.829918799167004	25.192246325526035	23.109184114519476
22	26.039730826242558	25.39869095252843	25.60849574067162	22.953082480557388
23	23.35044584530391	25.829558689292973	27.893594393003905	22.926401072399212
24	23.305634693112758	27.73594773317965	25.132606166371087	23.825811407336506
25	23.552466080064644	25.688280660106354	25.01337446775615	25.74587879207285
26	23.419199496170307	27.342581987796784	26.343074505306756	22.895144010726153
27	23.9672136868547	25.046835622267537	27.75493102296865	23.231019667909116
28	26.047066997993678	25.429753535421636	25.536354917722782	22.986824548861904
29	25.901640088170197	25.318106509220968	25.78441260142541	22.995840801183427
30	25.684996129602172	25.0500229133818	25.854900354745503	23.41008060227053
31	24.95566288983474	25.748369200892636	25.714319207853354	23.58164870141927
32	24.34917541267452	26.083380507942916	26.09096518035719	23.476478899025373
33	24.19930790404439	25.583683489675362	26.128667052707044	24.088341553573205
34	24.187990319494602	25.893455772851375	26.12644675330372	23.792107154350298
35	24.02800451212378	25.864343379945037	26.30988346021183	23.797768647719355
36	24.20926953932353	25.591975848760768	26.16889174700666	24.02986286490904
37	24.35040711161359	25.855159659785304	25.99444428147843	23.799988947122678
38	24.311311474674785	25.80055434015322	26.254700106428512	23.633434078743484
39	24.322877559887715	25.52905115910163	26.1466455354668	24.001425745543855
40	24.2879902276574	25.910813003953265	25.91807354506778	23.88312322332155
41	24.085224490907226	26.056256120341743	26.19080842505844	23.667710963692596
42	24.354912536680185	25.65882792933527	26.142388611063343	23.843870922921198
43	24.551941149424692	25.841076154810942	25.823848576228958	23.78313411953541
44	24.341234195830516	25.695843723767307	26.101985644548865	23.860936435853308
45	24.339797213734933	25.46312285273143	26.005399919410156	24.19168001412348
46	24.364425790328	25.822460213828336	25.838780224771014	23.974333771072654
47	24.367305156707488	25.682343655132506	26.10512971815649	23.845221470003512
48	24.24823552374243	25.389031839795727	26.167595221807638	24.1951374146542
49	24.27434429993771	25.953209377961244	25.8648727944013	23.907573527699746
50	24.214061280371578	25.843836673047193	26.213837953906044	23.72826409267519
51	24.370362795301848	25.49523886234884	26.030930661454203	24.103467680895115
52	24.332720347023614	25.77784894260537	25.793963670391527	24.09546703997949
53	24.244923982296594	25.78822654638586	25.937424183663165	24.029425287654373
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2766.0
1	1992.0
2	1218.0
3	1114.0
4	1010.0
5	1061.5
6	1113.0
7	1301.0
8	1731.0
9	1973.0
10	2841.5
11	3710.0
12	5540.5
13	7371.0
14	9144.5
15	10918.0
16	15418.0
17	19918.0
18	28110.5
19	36303.0
20	47843.0
21	59383.0
22	125365.0
23	191347.0
24	172096.5
25	173687.0
26	194528.0
27	223757.0
28	252986.0
29	288453.5
30	323921.0
31	354077.0
32	384233.0
33	426526.5
34	468820.0
35	525607.5
36	582395.0
37	647707.0
38	713019.0
39	772327.0
40	831635.0
41	899082.5
42	1029678.5
43	1092827.0
44	1153968.0
45	1215109.0
46	1271739.0
47	1328369.0
48	1361968.5
49	1395568.0
50	1406702.5
51	1417837.0
52	1386959.0
53	1356081.0
54	1315735.0
55	1275389.0
56	1198800.0
57	1122211.0
58	1019041.0
59	807577.5
60	699284.0
61	617099.0
62	534914.0
63	463147.0
64	391380.0
65	325235.5
66	259091.0
67	205489.0
68	151887.0
69	115201.5
70	78516.0
71	56852.5
72	35189.0
73	25761.0
74	16333.0
75	12240.0
76	5877.5
77	3608.0
78	2706.5
79	1805.0
80	1451.0
81	1097.0
82	837.5
83	578.0
84	425.5
85	273.0
86	183.0
87	93.0
88	76.0
89	59.0
90	47.0
91	35.0
92	27.0
93	14.5
94	10.0
95	7.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8511017E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.39739679005467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.80354262338501	33.778948548188524
2	13.878081021039106	12.87813663635357
3	5.021220338707059	6.989146572758526
4	2.3943388942452857	4.443643669046371
5	1.356877992766013	3.1477803313028816
6	0.8545066033364267	2.378812916083323
7	0.5756393238394749	1.869571628129723
8	0.441942020732272	1.6403967435291034
9	0.3170995299939034	1.3241333443560281
>10	2.140265856759604	19.239668909814736
>50	0.16387806889545478	5.089722563438174
>100	0.04633474321805133	3.705493804965781
>500	0.004023934601831664	1.2976306449004895
>1k	0.002190520209851472	1.57494458480748
>5k	2.341130821214706E-5	0.07315173888103756
>10k+	3.511696231821947E-5	0.568817363444021
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76368	0.4125543183283771	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1068534484085884E-4	0.0	0.0	1.080437665850558E-5	0.0
2	2.214897214993644E-4	0.0	0.0	1.620656498775837E-5	0.0
3	2.214897214993644E-4	0.0	0.0	2.160875331701116E-5	0.0
4	2.2689190982861718E-4	0.0	0.0	2.160875331701116E-5	5.40218832925279E-6
5	2.3229409815786998E-4	0.0	0.0	2.701094164626395E-5	1.080437665850558E-5
6	2.3769628648712278E-4	0.0	0.0	3.241312997551674E-5	1.620656498775837E-5
7	2.3769628648712278E-4	0.0	0.0	5.9424071621780696E-5	1.620656498775837E-5
8	2.3769628648712278E-4	0.0	0.0	7.022844828028627E-5	1.620656498775837E-5
9	2.4309847481637556E-4	1.080437665850558E-5	0.0	7.022844828028627E-5	2.701094164626395E-5
10	2.6470722813338673E-4	1.080437665850558E-5	0.0	7.563063660953907E-5	3.7815318304769535E-5
11	2.809137931211451E-4	1.080437665850558E-5	0.0	8.103282493879186E-5	4.8619694963275116E-5
12	3.025225464381563E-4	1.080437665850558E-5	0.0	9.183720159729743E-5	5.9424071621780696E-5
13	3.3493567641367303E-4	1.080437665850558E-5	0.0	9.183720159729743E-5	1.0264157825580302E-4
14	3.673488063891897E-4	1.080437665850558E-5	0.0	9.183720159729743E-5	1.1344595491430859E-4
15	3.889575597062009E-4	1.080437665850558E-5	0.0	9.723938992655023E-5	1.1884814324356139E-4
16	4.1596850135246487E-4	1.080437665850558E-5	0.0	1.1344595491430859E-4	1.2425033157281417E-4
17	4.6999038464499277E-4	1.080437665850558E-5	0.0	1.1344595491430859E-4	1.3505470823131977E-4
18	5.456210212545318E-4	1.080437665850558E-5	0.0	1.1344595491430859E-4	1.3505470823131977E-4
19	5.996429045470597E-4	1.620656498775837E-5	0.0	1.1344595491430859E-4	1.8907659152384766E-4
20	6.536647878395876E-4	1.620656498775837E-5	0.0	1.1344595491430859E-4	2.1068534484085884E-4
21	7.72512931083149E-4	2.701094164626395E-5	0.0	1.1884814324356139E-4	2.160875331701116E-4
22	9.02165450985216E-4	2.701094164626395E-5	0.0	1.2425033157281417E-4	2.214897214993644E-4
23	0.0010480245358750414	3.241312997551674E-5	0.0	1.2425033157281417E-4	2.2689190982861718E-4
24	0.001237101127398889	3.241312997551674E-5	0.0	1.2965251990206697E-4	2.3229409815786998E-4
25	0.001366753647300956	3.241312997551674E-5	0.0	1.4585908488982534E-4	2.4309847481637556E-4
26	0.00149100397887377	3.241312997551674E-5	0.0	1.5126127321907814E-4	2.4309847481637556E-4
27	0.0016476674404221011	3.241312997551674E-5	0.0	1.5126127321907814E-4	2.5930503980413393E-4
28	0.0018475484086044544	3.7815318304769535E-5	0.0	1.6746783820683651E-4	2.6470722813338673E-4
29	0.0020744403184330715	4.321750663402232E-5	0.0	1.6746783820683651E-4	2.6470722813338673E-4
30	0.0022527125332984137	4.321750663402232E-5	0.0	1.6746783820683651E-4	2.6470722813338673E-4
31	0.0025390285147488113	4.321750663402232E-5	0.0	1.6746783820683651E-4	2.6470722813338673E-4
32	0.002836148872857715	4.321750663402232E-5	0.0	1.8367440319459486E-4	2.7551160479189233E-4
33	0.003144073607625124	4.321750663402232E-5	0.0	1.9447877985310046E-4	2.863159814503979E-4
34	0.0034898136606973025	4.321750663402232E-5	0.0	1.9988096818235324E-4	2.917181697796507E-4
35	0.003765325265489195	4.321750663402232E-5	0.0	2.1068534484085884E-4	3.1872911142591463E-4
36	0.0041272718835491315	4.321750663402232E-5	0.0	2.160875331701116E-4	3.2953348808442023E-4
37	0.004548642573230849	4.8619694963275116E-5	0.0	2.2689190982861718E-4	3.2953348808442023E-4
38	0.004948404509595556	4.8619694963275116E-5	0.0	2.2689190982861718E-4	3.3493567641367303E-4
39	0.005434601459228307	4.8619694963275116E-5	0.0	2.3769628648712278E-4	3.565444297306842E-4
40	0.0059694181038243335	4.8619694963275116E-5	0.0	2.3769628648712278E-4	3.673488063891897E-4
41	0.006612278515005415	4.8619694963275116E-5	0.0	2.3769628648712278E-4	3.673488063891897E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	56755	0.0	40.519955	1
AGTGGTA	67885	0.0	33.710392	5
CAACGCA	68660	0.0	33.535244	13
GTGGTAT	68080	0.0	33.52754	6
TCAACGC	68705	0.0	33.4825	12
GGTATCA	68905	0.0	33.310284	8
GTATCAA	69090	0.0	33.289116	9
ACGCAGA	69405	0.0	33.15496	15
TGGTATC	69055	0.0	33.071175	7
AACGCAG	69595	0.0	33.064445	14
ATCAACG	70370	0.0	32.827198	11
TATCAAC	70265	0.0	32.7893	10
AGCAGTG	70950	0.0	32.575405	2
CAGTGGT	70985	0.0	32.4203	4
CGCAGAG	71110	0.0	32.40297	16
AGAGTAC	70175	0.0	32.39266	19
GCAGTGG	71905	0.0	32.110077	3
CAGAGTA	72625	0.0	31.332256	18
GCAGAGT	74310	0.0	30.805208	17
GTACATG	56810	0.0	30.151644	22
>>END_MODULE