FastQCFastQC Report
Thu 26 May 2016
SRR805237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21768233
Sequences flagged as poor quality0
Sequence length53
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5003312.2984456294638154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC763230.350616423482788No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG530690.24379103255647805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG361070.1658701466490183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3017850.045.5827671
AGCAGTG3150200.043.9392282
GTGGTAT3120850.043.9164666
AGTGGTA3131950.043.816345
GTATCAA3158150.043.689479
GGTATCA3154850.043.685268
TCAACGC3157500.043.64487512
TGGTATC3141050.043.634047
CAACGCA3159650.043.6174113
GCAGTGG3168900.043.6109663
TATCAAC3170500.043.5452310
ACGCAGA3163900.043.54247715
AACGCAG3165950.043.53729614
ATCAACG3185350.043.4728111
AGAGTAC3138000.043.2990119
CGCAGAG3180950.043.29800816
CAGTGGT3187000.043.1818964
CAGAGTA3152000.043.0403418
GCAGAGT3185050.042.77744317
GAGTACT815000.042.76711720