FastQCFastQC Report
Thu 26 May 2016
SRR805236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20573099
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1638510.7964332451810007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT973500.042.522091
TCAACGC999850.041.31685312
CAACGCA1004150.041.16800313
ATCAACG1012150.041.0074611
GGTATCA1014300.040.8510328
GTATCAA1017550.040.7505769
GTGGTAT1010750.040.7201586
AACGCAG1018100.040.60853614
TGGTATC1015500.040.5806057
AGTGGTA1017900.040.5172425
CGCAGAG1020000.040.4960316
TATCAAC1026300.040.4260510
AGAGTAC1017700.040.0402919
ACGCAGA1034100.039.94613615
CAGAGTA1032350.039.44021218
CAGTGGT1056300.039.1933634
GCAGTGG1064850.038.9691543
AGCAGTG1070300.038.867332
GCAGAGT1066300.038.36739717
GAGTACA762100.038.15011220
Produced by FastQC (version 0.11.5)