##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805235_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18128587
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.30995179050634	37.0	37.0	37.0	35.0	37.0
2	36.223495852158806	37.0	37.0	37.0	35.0	37.0
3	37.924956037665815	39.0	38.0	39.0	35.0	39.0
4	37.931435086474195	39.0	38.0	39.0	35.0	39.0
5	37.910076278973094	39.0	38.0	39.0	35.0	39.0
6	37.84868892429399	39.0	38.0	39.0	35.0	39.0
7	37.843306541210296	39.0	38.0	39.0	35.0	39.0
8	38.77803421744894	40.0	39.0	40.0	36.0	40.0
9	38.75587931922107	40.0	39.0	40.0	36.0	40.0
10	38.72652926562892	40.0	39.0	40.0	36.0	40.0
11	38.75886780365177	40.0	39.0	40.0	36.0	40.0
12	38.75836820597215	40.0	39.0	40.0	36.0	40.0
13	38.827756239358315	40.0	39.0	40.0	36.0	40.0
14	38.784610681461274	40.0	39.0	40.0	36.0	40.0
15	38.77176434103772	40.0	39.0	40.0	36.0	40.0
16	38.73540160631383	40.0	39.0	40.0	36.0	40.0
17	38.69833032215914	40.0	39.0	40.0	36.0	40.0
18	38.71577029141874	40.0	39.0	40.0	36.0	40.0
19	38.58771348257865	40.0	39.0	40.0	36.0	40.0
20	38.55980303373892	40.0	39.0	40.0	36.0	40.0
21	38.566234809144255	40.0	39.0	40.0	36.0	40.0
22	38.453167751022185	40.0	39.0	40.0	35.0	40.0
23	38.43079766779397	40.0	39.0	40.0	35.0	40.0
24	38.30619523738943	40.0	38.0	40.0	35.0	40.0
25	38.31070595849528	40.0	38.0	40.0	35.0	40.0
26	38.18379104780753	40.0	38.0	40.0	34.0	40.0
27	38.18828593756369	40.0	38.0	40.0	34.0	40.0
28	38.16963942087709	40.0	38.0	40.0	34.0	40.0
29	38.00174492363911	40.0	38.0	40.0	34.0	40.0
30	37.99071565809293	40.0	38.0	40.0	34.0	40.0
31	37.965096562683016	40.0	38.0	40.0	34.0	40.0
32	37.91339948336845	40.0	38.0	40.0	34.0	40.0
33	37.91349717437989	40.0	38.0	40.0	34.0	40.0
34	37.845991692568205	40.0	38.0	40.0	33.0	40.0
35	37.72741499378854	40.0	38.0	40.0	33.0	40.0
36	37.588229132253936	40.0	38.0	40.0	33.0	40.0
37	37.568491355669366	40.0	38.0	40.0	33.0	40.0
38	37.507424268642666	40.0	37.0	40.0	33.0	40.0
39	37.50390987449822	40.0	37.0	40.0	33.0	40.0
40	37.43671859257426	40.0	37.0	40.0	33.0	40.0
41	37.370446411515694	40.0	37.0	40.0	33.0	40.0
42	37.536786843894674	40.0	37.0	40.0	33.0	40.0
43	37.62499653172087	40.0	37.0	40.0	33.0	40.0
44	37.65147421583381	40.0	37.0	40.0	33.0	40.0
45	37.594369654954356	40.0	37.0	40.0	33.0	40.0
46	37.528439806147055	40.0	37.0	40.0	33.0	40.0
47	37.498183283672354	40.0	37.0	40.0	33.0	40.0
48	37.408064842560535	40.0	37.0	40.0	33.0	40.0
49	37.51104159414079	40.0	37.0	40.0	33.0	40.0
50	37.533802165607284	40.0	37.0	40.0	33.0	40.0
51	37.48752674436237	40.0	37.0	40.0	33.0	40.0
52	37.328972191820576	40.0	37.0	40.0	33.0	40.0
53	36.860342121534345	39.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	16.0
13	49.0
14	177.0
15	480.0
16	1043.0
17	1999.0
18	3133.0
19	4831.0
20	7222.0
21	10911.0
22	16323.0
23	24306.0
24	35426.0
25	49719.0
26	67856.0
27	90165.0
28	117194.0
29	149421.0
30	186349.0
31	232531.0
32	287744.0
33	359091.0
34	459525.0
35	620569.0
36	901279.0
37	1439216.0
38	3040270.0
39	1.0021736E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.227866573384897	29.19727279351667	22.745032472745947	24.829828160352484
2	25.398184646161337	23.54577331371717	29.15907897289513	21.896963067226366
3	20.91965579005137	25.694655628703988	27.526557916510537	25.859130664734103
4	21.940408262375882	29.37078328277874	26.293919101361844	22.394889353483535
5	25.907507297728166	27.780604191600812	24.86309054312948	21.44879796754154
6	24.475338315115238	26.17651337084352	27.454323936002297	21.89382437803895
7	24.03441592000524	27.055638699254388	27.048269123236135	21.86167625750424
8	23.2122779342924	26.163009836343008	27.39418135566771	23.230530873696885
9	22.656178333148635	26.23384271482383	28.34706863805767	22.762910313969865
10	22.428725415830808	27.055511827810957	27.729805968882186	22.785956787476046
11	22.4753313647666	27.276676334454525	27.40450207178309	22.843490228995783
12	22.5531587210851	26.692560208912035	27.467220694034232	23.28706037596863
13	22.657623564373772	27.17149990785272	26.700382109206856	23.470494418566652
14	22.971884129744915	27.424867696528143	26.674163849614974	22.92908432411197
15	23.015549970883004	26.887015518639153	26.59511742420962	23.502317086268224
16	23.35153864997862	26.763332409746	26.489416963384954	23.39571197689042
17	23.87129234065512	26.49339410732894	26.471042668686756	23.164270883329184
18	23.097006953713493	26.855049431045014	26.516280612493404	23.53166300274809
19	23.921649271396607	27.016098938102566	26.314952180222317	22.747299610278507
20	23.737961485911725	26.656633525823054	26.63936797721742	22.9660370110478
21	23.46867408916095	26.912560807965896	26.678824996123524	22.93994010674963
22	23.574440743782183	26.437476897675477	26.87437801964378	23.113704338898557
23	23.196601036804466	26.777177945528795	27.02252525251968	23.003695765147057
24	23.260334630603037	26.81997223501203	26.56924116590002	23.35045196848491
25	23.362455110263145	26.5806816604074	26.628275000142043	23.428588229187415
26	23.6177314867397	26.498915773192916	27.088553564599383	22.794799175468007
27	23.804210443979997	26.141491336307677	27.082927091890834	22.97137112782149
28	24.181509568285716	26.340414727303347	26.562511463248622	22.915564241162315
29	24.08483352839358	26.284789873584742	26.668713893697287	22.96166270432439
30	23.916546832910914	26.268020778453387	26.67768866928239	23.137743719353306
31	23.849602839978647	26.48905841365353	26.54579752961442	23.115541216753407
32	23.715146690693544	26.474462681509596	26.651282860600222	23.159107767196637
33	23.613539212956862	26.33934459425878	26.635230864931724	23.411885327852634
34	23.615138896373995	26.488650218574673	26.61597398627924	23.28023689877209
35	23.599754354820924	26.41311206438759	26.78557904154361	23.201554539247873
36	23.8944932663533	26.330380850973107	26.516175805648835	23.258950077024757
37	23.69736262401477	26.555263242524084	26.490089933650097	23.25728419981105
38	23.69299934959079	26.58007488393883	26.600076442802738	23.126849323667642
39	23.682849634116547	26.50266675499861	26.536662785687597	23.277820825197242
40	23.68801826639881	26.60788179464842	26.498689611054626	23.205410327898143
41	23.637159366033327	26.50369827499518	26.652711543376217	23.20643081559528
42	23.592677134737528	26.398075040266516	26.702847828129133	23.306399996866826
43	23.688310622333667	26.451984371423983	26.51008597636429	23.34961902987806
44	23.69613252262849	26.32278511281657	26.680507421786377	23.300574942768566
45	23.61463692675	26.271479404324232	26.714674453116505	23.399209215809265
46	23.62417986575567	26.471197120878752	26.553911785844093	23.350711227521483
47	23.615795318189996	26.368878059828933	26.668840765140718	23.34648585684036
48	23.532727619642944	26.337127102073648	26.643163088220827	23.48698219006258
49	23.71019870439985	26.50445950365575	26.498700643354056	23.286641148590345
50	23.735473702390593	26.402995445811634	26.636229288030005	23.22530156376777
51	23.668375257266327	26.31765509358231	26.66504013798759	23.348929511163778
52	23.768046566453304	26.427762958028666	26.468152206236482	23.336038269281552
53	23.720905550995234	26.432567524429786	26.535918105476174	23.310608819098807
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5234.0
1	3426.5
2	1619.0
3	1526.5
4	1434.0
5	1554.5
6	1675.0
7	1878.5
8	2459.0
9	2836.0
10	3546.0
11	4256.0
12	5666.5
13	7077.0
14	9439.0
15	11801.0
16	16709.5
17	21618.0
18	30207.5
19	38797.0
20	52792.0
21	66787.0
22	101147.5
23	135508.0
24	142615.0
25	178869.0
26	208016.0
27	245525.0
28	283034.0
29	325171.5
30	367309.0
31	406338.0
32	445367.0
33	496541.5
34	547716.0
35	613820.5
36	679925.0
37	746590.0
38	813255.0
39	876739.5
40	940224.0
41	1008100.0
42	1131478.5
43	1186981.0
44	1237630.0
45	1288279.0
46	1315849.0
47	1343419.0
48	1357116.5
49	1370814.0
50	1364461.5
51	1358109.0
52	1307361.5
53	1256614.0
54	1193600.0
55	1130586.0
56	1040862.0
57	951138.0
58	851928.5
59	656480.5
60	560242.0
61	487541.0
62	414840.0
63	354301.5
64	293763.0
65	240419.5
66	187076.0
67	149363.0
68	111650.0
69	85792.0
70	59934.0
71	43912.0
72	27890.0
73	19941.0
74	11992.0
75	8868.5
76	4254.0
77	2763.0
78	2027.0
79	1291.0
80	1036.5
81	782.0
82	587.5
83	393.0
84	271.0
85	149.0
86	108.0
87	67.0
88	52.0
89	37.0
90	31.5
91	26.0
92	18.5
93	8.5
94	6.0
95	3.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8128587E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.573100387721986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.41111572359732	46.51355253179273
2	11.582071107008247	13.567956272970566
3	3.804159384121592	6.68464228491146
4	1.6879088738491552	3.9546422365317335
5	0.9299362768206251	2.7234625448199448
6	0.5800630411762787	2.038565444521529
7	0.36231594188005317	1.4855377625068678
8	0.25833774100945067	1.2105313950467103
9	0.21733467008099622	1.1456968903547966
>10	1.0288438363768408	10.687914973885652
>50	0.08720142760426415	3.6338303433099504
>100	0.04847105508732281	5.190602636871279
>500	0.0020342147314489347	0.7631273139846799
>1k	1.879219658293226E-4	0.24512281266968225
>5k	0.0	0.0
>10k+	1.87846906703315E-5	0.15481455582252027
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.69582030855466E-4	0.0	0.0	0.0	5.516149714260686E-6
2	3.750981805697267E-4	0.0	0.0	0.0	5.516149714260686E-6
3	3.750981805697267E-4	0.0	0.0	0.0	1.654844914278206E-5
4	3.806143302839874E-4	0.0	0.0	0.0	2.7580748571303434E-5
5	3.806143302839874E-4	0.0	0.0	0.0	4.412919771408549E-5
6	3.806143302839874E-4	0.0	0.0	5.516149714260686E-6	4.964534742834618E-5
7	3.806143302839874E-4	0.0	0.0	5.516149714260686E-6	5.516149714260687E-5
8	3.8613047999824806E-4	0.0	0.0	5.516149714260686E-6	6.067764685686755E-5
9	3.8613047999824806E-4	0.0	0.0	5.516149714260686E-6	8.274224571391031E-5
10	3.8613047999824806E-4	0.0	0.0	5.516149714260686E-6	8.274224571391031E-5
11	3.9716277942676944E-4	0.0	0.0	5.516149714260686E-6	8.825839542817098E-5
12	4.0267892914103013E-4	0.0	0.0	5.516149714260686E-6	9.929069485669236E-5
13	4.137112285695515E-4	0.0	0.0	5.516149714260686E-6	1.0480684457095305E-4
14	4.137112285695515E-4	0.0	0.0	5.516149714260686E-6	1.213552937137351E-4
15	4.3577582742659426E-4	0.0	0.0	1.1032299428521373E-5	1.213552937137351E-4
16	4.57840426283637E-4	0.0	0.0	1.1032299428521373E-5	1.5445219199929924E-4
17	4.909373245692012E-4	0.0	0.0	1.1032299428521373E-5	1.6548449142782061E-4
18	5.074857737119832E-4	0.0	0.0	1.1032299428521373E-5	1.6548449142782061E-4
19	5.571311211403293E-4	0.0	0.0	1.1032299428521373E-5	1.7100064114208128E-4
20	6.122926182829362E-4	0.0	0.0	1.1032299428521373E-5	1.8203294057060265E-4
21	6.729702651398038E-4	0.0	0.0	1.654844914278206E-5	1.8754909028486334E-4
22	7.281317622824106E-4	5.516149714260686E-6	0.0	1.654844914278206E-5	2.096136891419061E-4
23	8.053578582820603E-4	1.1032299428521373E-5	5.516149714260686E-6	1.654844914278206E-5	2.2064598857042748E-4
24	8.991324034244919E-4	1.1032299428521373E-5	5.516149714260686E-6	1.654844914278206E-5	2.2616213828468816E-4
25	9.929069485669236E-4	1.1032299428521373E-5	5.516149714260686E-6	1.654844914278206E-5	2.2616213828468816E-4
26	0.001108746092566398	1.1032299428521373E-5	5.516149714260686E-6	1.654844914278206E-5	2.427105874274702E-4
27	0.0012135529371373511	1.1032299428521373E-5	5.516149714260686E-6	2.7580748571303434E-5	2.482267371417309E-4
28	0.0013955858777079538	1.1032299428521373E-5	5.516149714260686E-6	3.309689828556412E-5	2.537428868559916E-4
29	0.001566586518850035	1.1032299428521373E-5	5.516149714260686E-6	3.309689828556412E-5	2.6477518628451296E-4
30	0.001743103309706377	1.1032299428521373E-5	5.516149714260686E-6	3.309689828556412E-5	2.7029133599877365E-4
31	0.001936168549705501	1.1032299428521373E-5	5.516149714260686E-6	3.861304799982481E-5	2.7029133599877365E-4
32	0.0021733629874187105	1.1032299428521373E-5	5.516149714260686E-6	3.861304799982481E-5	2.978720845700771E-4
33	0.0024381381737032235	1.1032299428521373E-5	5.516149714260686E-6	3.861304799982481E-5	3.1442053371285916E-4
34	0.002697397210273476	1.1032299428521373E-5	5.516149714260686E-6	3.861304799982481E-5	3.1442053371285916E-4
35	0.0029732046959865103	1.1032299428521373E-5	5.516149714260686E-6	3.861304799982481E-5	3.4751743199842324E-4
36	0.003326238277699194	1.1032299428521373E-5	5.516149714260686E-6	3.861304799982481E-5	3.4751743199842324E-4
37	0.00369582030855466	1.1032299428521373E-5	5.516149714260686E-6	3.861304799982481E-5	3.4751743199842324E-4
38	0.004153660734838297	1.654844914278206E-5	5.516149714260686E-6	3.861304799982481E-5	3.585497314269446E-4
39	0.00457840426283637	1.654844914278206E-5	5.516149714260686E-6	3.861304799982481E-5	3.585497314269446E-4
40	0.00514105153369096	1.654844914278206E-5	5.516149714260686E-6	3.861304799982481E-5	3.750981805697267E-4
41	0.00570369880454555	1.654844914278206E-5	5.516149714260686E-6	3.861304799982481E-5	3.8613047999824806E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	17495	0.0	26.1126	1
AGCAGTG	29120	0.0	15.712396	2
GCAGTGG	31870	0.0	14.445088	3
AGTGGTA	32115	0.0	14.203176	5
TCAACGC	32600	0.0	13.919784	12
CAACGCA	32670	0.0	13.86838	13
GGTATCA	33430	0.0	13.616362	8
ATCAACG	33515	0.0	13.581828	11
GTGGTAT	33695	0.0	13.558093	6
GTATCAA	33845	0.0	13.470231	9
AACGCAG	33755	0.0	13.436527	14
TGGTATC	34310	0.0	13.280821	7
ACGCAGA	34355	0.0	13.201862	15
TATCAAC	34680	0.0	13.125576	10
CGCAGAG	34535	0.0	13.099029	16
CAGTGGT	35740	0.0	12.933547	4
AGAGTAC	34765	0.0	12.7622595	19
CAGAGTA	36505	0.0	12.263388	18
GAGTACT	9420	0.0	11.974522	20
GCAGAGT	38870	0.0	11.632106	17
>>END_MODULE