FastQCFastQC Report
Thu 26 May 2016
SRR805234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11012202
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT419150.3806232395664373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT267150.038.0978851
AGTGGTA332550.030.6266735
TCAACGC335050.030.51738712
CAACGCA335900.030.34221513
GGTATCA337750.030.169068
GTGGTAT338950.030.0691816
ATCAACG341350.029.97480411
GTATCAA339550.029.9676079
AGCAGTG344800.029.818012
TGGTATC341800.029.8115867
AACGCAG342000.029.77353714
GCAGTGG347050.029.5637533
ACGCAGA345300.029.46857815
CGCAGAG347450.029.23888416
TATCAAC348200.029.22989710
AGAGTAC342500.029.0302219
CAGTGGT355700.028.6994674
CAGAGTA356150.028.02976218
GCAGAGT370600.027.15245617
GAGTACT100450.026.76356520