##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805234_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11012202
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.779332961745524	37.0	35.0	37.0	35.0	37.0
2	35.85686595650897	37.0	35.0	37.0	35.0	37.0
3	37.53630454653847	39.0	37.0	39.0	35.0	39.0
4	37.57023999378144	39.0	37.0	39.0	35.0	39.0
5	37.59227373417233	39.0	37.0	39.0	35.0	39.0
6	37.55106263034405	39.0	37.0	39.0	35.0	39.0
7	37.526246975854605	39.0	37.0	39.0	35.0	39.0
8	38.4198451862761	40.0	38.0	40.0	35.0	40.0
9	38.40416394468608	40.0	38.0	40.0	35.0	40.0
10	38.332953754389905	40.0	38.0	40.0	34.0	40.0
11	38.38728748346607	40.0	38.0	40.0	35.0	40.0
12	38.422832508884234	40.0	38.0	40.0	35.0	40.0
13	38.49894998293711	40.0	39.0	40.0	35.0	40.0
14	38.43372551647709	40.0	39.0	40.0	35.0	40.0
15	38.425537508302156	40.0	39.0	40.0	35.0	40.0
16	38.361005183159556	40.0	38.0	40.0	34.0	40.0
17	38.30219242255091	40.0	38.0	40.0	34.0	40.0
18	38.337655720445376	40.0	38.0	40.0	34.0	40.0
19	38.25293860392318	40.0	38.0	40.0	34.0	40.0
20	38.15056271216238	40.0	38.0	40.0	34.0	40.0
21	38.133886210950365	40.0	38.0	40.0	34.0	40.0
22	37.98518080216836	40.0	38.0	40.0	34.0	40.0
23	37.90825595098964	40.0	38.0	40.0	34.0	40.0
24	37.792045859674566	40.0	38.0	40.0	33.0	40.0
25	37.841677077845105	40.0	38.0	40.0	34.0	40.0
26	37.67416798202576	40.0	38.0	40.0	33.0	40.0
27	37.71823355583198	40.0	38.0	40.0	33.0	40.0
28	37.71325280811231	40.0	38.0	40.0	33.0	40.0
29	37.53185103215506	40.0	38.0	40.0	33.0	40.0
30	37.500314015307744	40.0	38.0	40.0	33.0	40.0
31	37.47643250641425	40.0	38.0	40.0	33.0	40.0
32	37.435526881907904	40.0	37.0	40.0	32.0	40.0
33	37.415308491435226	40.0	37.0	40.0	32.0	40.0
34	37.34501174242899	40.0	37.0	40.0	32.0	40.0
35	37.234372289938015	40.0	37.0	40.0	31.0	40.0
36	37.09891591164056	40.0	37.0	40.0	31.0	40.0
37	37.05508044621775	40.0	37.0	40.0	31.0	40.0
38	36.982788183507715	40.0	37.0	40.0	31.0	40.0
39	36.96628567111283	40.0	37.0	40.0	31.0	40.0
40	36.88119079181439	40.0	36.0	40.0	31.0	40.0
41	36.81477428401695	39.0	36.0	40.0	31.0	40.0
42	36.94731108274258	40.0	37.0	40.0	31.0	40.0
43	36.98572501666787	40.0	36.0	40.0	31.0	40.0
44	37.015332900722306	40.0	36.0	40.0	31.0	40.0
45	36.986641636250404	40.0	36.0	40.0	31.0	40.0
46	36.83217434623884	40.0	36.0	40.0	31.0	40.0
47	36.81116120100231	39.0	36.0	40.0	31.0	40.0
48	36.7148581182946	39.0	36.0	40.0	31.0	40.0
49	36.844041091872455	39.0	36.0	40.0	31.0	40.0
50	36.7634229738975	39.0	36.0	40.0	31.0	40.0
51	36.72813993059699	39.0	36.0	40.0	31.0	40.0
52	36.53867509876771	39.0	35.0	40.0	30.0	40.0
53	36.066844669213296	39.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	61.0
11	160.0
12	328.0
13	606.0
14	997.0
15	1441.0
16	2292.0
17	3344.0
18	4601.0
19	6420.0
20	8798.0
21	12868.0
22	18076.0
23	25697.0
24	35203.0
25	47932.0
26	63199.0
27	81153.0
28	102533.0
29	127785.0
30	155273.0
31	188261.0
32	227010.0
33	279016.0
34	347664.0
35	457440.0
36	642101.0
37	1000185.0
38	1907263.0
39	5264484.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	18.17842607681915	32.53697126151518	23.935721484222682	25.348881177442983
2	27.997134451402182	26.298890993826667	25.377958014210055	20.3260165405611
3	23.104271062227156	24.69240938370001	26.049712854885883	26.153606699186955
4	21.031760950262264	30.262775782718116	23.553672553409392	25.15179071361023
5	25.71339501400356	29.395991827974093	23.401032781636225	21.489580376386122
6	26.113097089937142	25.895175188395562	25.882434775533543	22.109292946133753
7	24.09815039716852	26.83244459191722	27.37842985444691	21.690975156467342
8	24.082667571844397	26.025539669541114	26.582676198638566	23.309116559975926
9	23.680450104347887	25.72174030225744	27.70424116811515	22.893568425279522
10	22.091358295098473	26.956588700425222	28.130023404946623	22.822029599529685
11	22.091004142495752	28.491413433934465	26.630214374927014	22.78736804864277
12	22.17524705776374	26.354456629110146	28.11217048143505	23.358125831691066
13	22.30335949158942	26.986164983170486	25.97493217069574	24.73554335454435
14	22.736370073850807	28.640829508939262	25.816289966348236	22.80651045086169
15	22.559693329272385	28.150364477513218	25.83002019033069	23.459922002883708
16	23.125374925014995	26.45589864770007	25.746585469463785	24.67214095782115
17	25.05639653177448	26.0515925879311	25.70635736612895	23.18565351416547
18	22.817625394085578	26.46386254084333	25.739529659917242	24.978982405153847
19	23.469011919686906	28.12923337221747	25.627199719002615	22.77455498909301
20	24.919884324679114	26.413545628748906	25.85366668718936	22.81290335938262
21	23.334297718113053	27.957514764077157	25.868922491614306	22.839265026195488
22	24.68076775199002	26.15285298980168	26.27511736526446	22.891261892943845
23	22.973407135103407	26.451240178848877	27.814073879138796	22.76127880690892
24	22.98516681768097	27.835559137037265	25.886675525930237	23.292598519351536
25	22.863011412249794	26.421963563690532	25.961864847738898	24.753160176320776
26	23.15290801966764	27.244387634734636	26.93247000009626	22.670234345501473
27	23.410994458692276	25.820666929284442	27.96821198884655	22.800126623176727
28	24.72036927764311	26.040695584770425	26.43081737875858	22.80811775882789
29	24.804566788731265	25.953791984564035	26.495373041649618	22.746268185055087
30	24.593982202651205	25.90105957010233	26.501048564129135	23.00390966311733
31	23.951740078868877	26.36084045679511	26.541730709262325	23.14568875507369
32	23.815509377688496	26.41125725808517	26.70391443963705	23.06931892458929
33	23.643373051093686	26.237776967767207	26.70197114074006	23.41687884039904
34	23.5092491038577	26.430426902811988	26.69906527323055	23.36125872009976
35	23.53794454551415	26.328721539979018	26.846211139243543	23.287122775263295
36	23.691437915868235	26.2682885766171	26.710053084750896	23.330220422763766
37	23.6301241114175	26.514551767212406	26.62924272547852	23.226081395891576
38	23.696713881565195	26.412047290814318	26.774109301663735	23.117129525956752
39	23.6290979769532	26.31484602262109	26.7503992389533	23.30565676147241
40	23.530743442592136	26.57881684335249	26.64484360167022	23.245596112385154
41	23.596788362581798	26.455135857478822	26.79090884820311	23.157166931736267
42	23.509167376334	26.37908385625327	26.812276055234	23.299472712178726
43	23.49463803878643	26.474341825549512	26.770349835573302	23.260670300090755
44	23.638623773882824	26.246975854602013	26.83136397243712	23.283036399078043
45	23.582168216674557	26.16188842158907	26.84244259231714	23.413500769419233
46	23.496917328614206	26.38581275570499	26.69542385800769	23.421846057673115
47	23.575230457995595	26.298927317170534	26.786540966102873	23.339301258730995
48	23.496009245017483	26.19322638651198	26.76774363565071	23.54302073281983
49	23.477711360543513	26.51703083543146	26.665220997580686	23.340036806444342
50	23.6967956090889	26.36310158495095	26.732827821356707	23.207274984603444
51	23.574939871244645	26.254749050189965	26.789937198754615	23.38037387981078
52	23.644045032955262	26.400868781738655	26.627680821692156	23.327405363613927
53	23.740637885138685	26.31898688382214	26.59628837175344	23.344086859285724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2039.0
1	1300.5
2	562.0
3	489.0
4	416.0
5	420.0
6	424.0
7	510.5
8	749.5
9	902.0
10	1220.5
11	1539.0
12	2251.5
13	2964.0
14	4142.0
15	5320.0
16	7970.5
17	10621.0
18	15148.5
19	19676.0
20	27613.0
21	35550.0
22	73925.5
23	112301.0
24	101950.0
25	107719.5
26	123840.0
27	144645.5
28	165451.0
29	190357.5
30	215264.0
31	237966.0
32	260668.0
33	292541.0
34	324414.0
35	365015.0
36	405616.0
37	448910.5
38	492205.0
39	531827.5
40	571450.0
41	612485.5
42	686265.5
43	719010.0
44	747087.0
45	775164.0
46	790239.0
47	805314.0
48	814398.0
49	823482.0
50	818969.5
51	814457.0
52	787017.0
53	759577.0
54	724769.5
55	689962.0
56	642699.5
57	595437.0
58	533560.0
59	412673.5
60	353664.0
61	308448.5
62	263233.0
63	224775.5
64	186318.0
65	152100.0
66	117882.0
67	93924.5
68	69967.0
69	53300.5
70	36634.0
71	27183.5
72	17733.0
73	12890.0
74	8047.0
75	5941.5
76	2786.5
77	1737.0
78	1310.5
79	884.0
80	748.0
81	612.0
82	479.0
83	346.0
84	237.0
85	128.0
86	97.5
87	67.0
88	52.0
89	37.0
90	33.5
91	30.0
92	19.5
93	8.5
94	8.0
95	5.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.1012202E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.43837928480572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.2063928471474	52.97246721920224
2	10.587703827169898	13.64508949940726
3	3.2205618828461255	6.2258336435108
4	1.3170497930239171	3.3947421639940014
5	0.7258765264418203	2.338715346239766
6	0.44028945905350947	1.7022923494555187
7	0.27954321697604406	1.2609318289397944
8	0.20961041598630642	1.080556438989721
9	0.15713854752141926	0.9113177992903813
>10	0.7434514505527645	8.878096382872476
>50	0.08319200556605388	3.6935746944082344
>100	0.02868262792498265	3.095338896577694
>500	3.105086743749253E-4	0.12666961642729793
>1k	1.5470044348036112E-4	0.17491295183159283
>5k	2.812711469862265E-5	0.1196136728906216
>10k+	1.4063557349311325E-5	0.37984749596260353
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41915	0.3806232395664373	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.543742114429067E-4	0.0	0.0	0.0	0.0
2	1.543742114429067E-4	0.0	0.0	0.0	0.0
3	1.543742114429067E-4	0.0	0.0	9.080835967229806E-6	9.080835967229806E-6
4	1.543742114429067E-4	0.0	0.0	1.8161671934459612E-5	9.080835967229806E-6
5	1.543742114429067E-4	0.0	0.0	2.7242507901689416E-5	9.080835967229806E-6
6	1.543742114429067E-4	0.0	0.0	2.7242507901689416E-5	9.080835967229806E-6
7	1.634550474101365E-4	0.0	0.0	3.6323343868919224E-5	1.8161671934459612E-5
8	1.634550474101365E-4	0.0	0.0	4.5404179836149025E-5	2.7242507901689416E-5
9	1.634550474101365E-4	0.0	0.0	4.5404179836149025E-5	2.7242507901689416E-5
10	1.7253588337736632E-4	0.0	0.0	4.5404179836149025E-5	2.7242507901689416E-5
11	1.7253588337736632E-4	0.0	0.0	4.5404179836149025E-5	5.448501580337883E-5
12	1.7253588337736632E-4	0.0	0.0	5.448501580337883E-5	5.448501580337883E-5
13	1.906975553118259E-4	0.0	0.0	6.356585177060864E-5	7.264668773783845E-5
14	1.906975553118259E-4	9.080835967229806E-6	0.0	6.356585177060864E-5	8.172752370506826E-5
15	2.1794006321351533E-4	1.8161671934459612E-5	0.0	6.356585177060864E-5	8.172752370506826E-5
16	2.1794006321351533E-4	1.8161671934459612E-5	0.0	7.264668773783845E-5	9.988919563952786E-5
17	2.2702089918074514E-4	1.8161671934459612E-5	0.0	8.172752370506826E-5	1.0897003160675767E-4
18	2.4518257111520475E-4	1.8161671934459612E-5	0.0	8.172752370506826E-5	1.0897003160675767E-4
19	2.5426340708243456E-4	1.8161671934459612E-5	0.0	9.080835967229805E-5	1.0897003160675767E-4
20	2.6334424304966437E-4	1.8161671934459612E-5	0.0	9.988919563952786E-5	1.1805086757398747E-4
21	2.81505914984124E-4	1.8161671934459612E-5	0.0	9.988919563952786E-5	1.362125395084471E-4
22	3.3599093078750283E-4	1.8161671934459612E-5	0.0	1.1805086757398747E-4	1.452933754756769E-4
23	3.813951106236518E-4	1.8161671934459612E-5	0.0	1.1805086757398747E-4	1.816167193445961E-4
24	4.1771845449257105E-4	1.8161671934459612E-5	0.0	1.1805086757398747E-4	1.816167193445961E-4
25	4.631226343287201E-4	1.8161671934459612E-5	0.0	1.362125395084471E-4	1.816167193445961E-4
26	5.085268141648691E-4	1.8161671934459612E-5	0.0	1.362125395084471E-4	1.816167193445961E-4
27	5.811735019027076E-4	1.8161671934459612E-5	0.0	1.362125395084471E-4	1.816167193445961E-4
28	6.901435335094653E-4	1.8161671934459612E-5	0.0	1.452933754756769E-4	1.816167193445961E-4
29	7.537093852800739E-4	2.7242507901689416E-5	0.0	1.634550474101365E-4	1.9977839127905572E-4
30	8.081944010834527E-4	2.7242507901689416E-5	0.0	1.634550474101365E-4	2.0885922724628552E-4
31	8.808410888212912E-4	2.7242507901689416E-5	0.0	1.7253588337736632E-4	2.0885922724628552E-4
32	0.0010624578081658872	3.6323343868919224E-5	0.0	1.816167193445961E-4	2.2702089918074514E-4
33	0.0011351044959037257	4.5404179836149025E-5	0.0	1.816167193445961E-4	2.4518257111520475E-4
34	0.0012803978713794026	7.264668773783845E-5	0.0	1.906975553118259E-4	2.4518257111520475E-4
35	0.0014166104108878496	8.172752370506826E-5	0.0	2.0885922724628552E-4	2.724250790168942E-4
36	0.001589146294265216	9.080835967229805E-5	0.0	2.1794006321351533E-4	2.996675869185836E-4
37	0.0016981163258719737	9.988919563952786E-5	0.0	2.1794006321351533E-4	3.087484228858134E-4
38	0.0018978947171510293	9.988919563952786E-5	0.0	2.4518257111520475E-4	3.178292588530432E-4
39	0.0020795114364956257	9.988919563952786E-5	0.0	2.5426340708243456E-4	3.178292588530432E-4
40	0.00233377484357806	9.988919563952786E-5	0.0	2.724250790168942E-4	3.26910094820273E-4
41	0.002479068219053737	9.988919563952786E-5	0.0	2.724250790168942E-4	3.3599093078750283E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	26715	0.0	38.097885	1
AGTGGTA	33255	0.0	30.626673	5
TCAACGC	33505	0.0	30.517387	12
CAACGCA	33590	0.0	30.342215	13
GGTATCA	33775	0.0	30.16906	8
GTGGTAT	33895	0.0	30.069181	6
ATCAACG	34135	0.0	29.974804	11
GTATCAA	33955	0.0	29.967607	9
AGCAGTG	34480	0.0	29.81801	2
TGGTATC	34180	0.0	29.811586	7
AACGCAG	34200	0.0	29.773537	14
GCAGTGG	34705	0.0	29.563753	3
ACGCAGA	34530	0.0	29.468578	15
CGCAGAG	34745	0.0	29.238884	16
TATCAAC	34820	0.0	29.229897	10
AGAGTAC	34250	0.0	29.03022	19
CAGTGGT	35570	0.0	28.699467	4
CAGAGTA	35615	0.0	28.029762	18
GCAGAGT	37060	0.0	27.152456	17
GAGTACT	10045	0.0	26.763565	20
>>END_MODULE