FastQCFastQC Report
Thu 26 May 2016
SRR805233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21795957
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3355231.5393818220507591No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG279280.1281338552833445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1414800.042.9670641
TCAACGC1472700.041.6639512
CAACGCA1475650.041.58702513
GTGGTAT1470350.041.5211646
ATCAACG1478550.041.5022811
GGTATCA1474850.041.4821178
TGGTATC1478600.041.3022087
AGTGGTA1479750.041.2764665
GTATCAA1483400.041.2509429
AACGCAG1491850.041.1559114
CGCAGAG1492800.041.07147616
TATCAAC1490200.041.0500910
ACGCAGA1497850.040.95339615
AGAGTAC1475850.040.43811819
CAGAGTA1504250.040.1808218
GCAGTGG1529050.040.1100653
AGCAGTG1531250.040.0800632
CAGTGGT1526100.040.0551724
GAGTACT572650.039.5599420
GCAGAGT1539650.039.45081717
Produced by FastQC (version 0.11.5)