##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805233_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21795957
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.52247341100921	35.0	35.0	37.0	35.0	37.0
2	35.99556036929234	37.0	35.0	37.0	35.0	37.0
3	37.89864083508699	39.0	38.0	39.0	35.0	39.0
4	37.88476037092567	39.0	38.0	39.0	35.0	39.0
5	37.842181143961696	39.0	38.0	39.0	35.0	39.0
6	37.7520670003157	39.0	38.0	39.0	35.0	39.0
7	37.74802253463796	39.0	38.0	39.0	35.0	39.0
8	38.761404787135525	40.0	39.0	40.0	36.0	40.0
9	38.79620559904757	40.0	39.0	40.0	36.0	40.0
10	38.55685593433682	40.0	38.0	40.0	36.0	40.0
11	38.67351119292445	40.0	39.0	40.0	36.0	40.0
12	38.776170186057904	40.0	39.0	40.0	36.0	40.0
13	38.80735395101027	40.0	39.0	40.0	36.0	40.0
14	38.80762803853944	40.0	39.0	40.0	36.0	40.0
15	38.7646591062737	40.0	39.0	40.0	36.0	40.0
16	38.75121115351806	40.0	39.0	40.0	36.0	40.0
17	38.74020879193329	40.0	39.0	40.0	36.0	40.0
18	38.725676739039265	40.0	39.0	40.0	36.0	40.0
19	38.653977203203326	40.0	39.0	40.0	36.0	40.0
20	38.60745242798928	40.0	39.0	40.0	36.0	40.0
21	38.513425586222255	40.0	39.0	40.0	36.0	40.0
22	38.45871755023191	40.0	39.0	40.0	35.0	40.0
23	38.14077642931669	40.0	38.0	40.0	34.0	40.0
24	38.226790546522	40.0	38.0	40.0	35.0	40.0
25	38.03040871295534	40.0	38.0	40.0	34.0	40.0
26	38.0583396728118	40.0	38.0	40.0	34.0	40.0
27	38.09949074500376	40.0	38.0	40.0	34.0	40.0
28	38.084266683036674	40.0	38.0	40.0	34.0	40.0
29	38.019484347487015	40.0	38.0	40.0	34.0	40.0
30	37.95564879303075	40.0	38.0	40.0	34.0	40.0
31	37.72753245016954	40.0	38.0	40.0	33.0	40.0
32	37.78618305220551	40.0	38.0	40.0	33.0	40.0
33	37.7225224384504	40.0	38.0	40.0	33.0	40.0
34	37.62832579454988	40.0	38.0	40.0	33.0	40.0
35	37.69494347047941	40.0	38.0	40.0	33.0	40.0
36	37.691693601707875	40.0	38.0	40.0	33.0	40.0
37	37.834804454789484	40.0	38.0	40.0	33.0	40.0
38	37.86663109126156	40.0	38.0	40.0	33.0	40.0
39	37.94446025012804	40.0	38.0	40.0	34.0	40.0
40	37.90758056643257	40.0	38.0	40.0	34.0	40.0
41	37.87704811493251	40.0	38.0	40.0	34.0	40.0
42	37.867131367528394	40.0	38.0	40.0	34.0	40.0
43	37.89474492907102	40.0	38.0	40.0	34.0	40.0
44	37.863424028593926	40.0	38.0	40.0	34.0	40.0
45	37.66286495243131	40.0	37.0	40.0	33.0	40.0
46	37.712058066548764	40.0	37.0	40.0	33.0	40.0
47	37.64975949438696	40.0	37.0	40.0	33.0	40.0
48	37.67716104413309	40.0	37.0	40.0	33.0	40.0
49	37.490229265913854	40.0	37.0	40.0	33.0	40.0
50	37.413020038532835	40.0	37.0	40.0	33.0	40.0
51	37.28239214272629	40.0	36.0	40.0	33.0	40.0
52	36.80507559268905	40.0	36.0	40.0	31.0	40.0
53	36.42382901562891	39.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	33.0
13	83.0
14	223.0
15	616.0
16	1340.0
17	2362.0
18	3548.0
19	5382.0
20	8110.0
21	12769.0
22	19412.0
23	28988.0
24	42199.0
25	58935.0
26	80185.0
27	106305.0
28	137459.0
29	173720.0
30	218263.0
31	270654.0
32	334726.0
33	420086.0
34	548480.0
35	755052.0
36	1133178.0
37	2006176.0
38	3722062.0
39	1.1705604E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.657414904975267	46.5093732750528	30.50032168810023	13.332890131871705
2	30.73499823843477	45.02334997265777	14.029854252327622	10.211797536579835
3	37.28118935085071	25.81285143845714	20.96528727781946	15.940671932872688
4	23.27398608833739	27.486138828407487	19.893785806239205	29.34608927701592
5	19.325519865909076	34.833556516926514	24.873218459735448	20.967705157428966
6	27.793237066856026	25.22127842333328	23.223426252859646	23.76205825695105
7	22.456196807508842	24.98393624101938	30.448568970841706	22.111297980630077
8	25.76604918058886	26.623877997190032	26.15388716356891	21.4561856586522
9	27.474008138298313	25.505193463173008	25.894430788242058	21.12636761028662
10	21.526299579321066	26.902383776954597	30.807984251391208	20.76333239233313
11	20.92229765364283	32.42863802676799	25.87863886866725	20.77042545092193
12	21.016067337625966	26.03585609936742	31.417225680891185	21.53085088211543
13	21.030849895693958	26.66713831377076	25.62414212874434	26.677869661790947
14	21.225349270050405	32.614209139795975	25.210831531737742	20.949610058415878
15	21.19310016990766	32.1191540247579	25.08259215229687	21.60515365303758
16	21.831374506749118	26.042921629915128	25.249182680989872	26.87652118234588
17	27.367860011836143	25.716402358474095	25.401431100272404	21.51430652941736
18	21.453850363165976	25.87197249471542	25.217218037271778	27.456959104846828
19	21.718174613759793	31.849342517972485	25.129105365733658	21.303377502534072
20	27.660244512319416	26.329139849193133	25.058890508914107	20.951725129573344
21	22.165124476984424	31.490317218005153	25.043571154044763	21.300987150965657
22	27.249016870422345	25.931570703686006	25.643485165620394	21.17592726027125
23	21.736682633389304	26.068940216756715	31.156617715845194	21.037759434008795
24	21.83490727202297	31.297134601614417	25.07952277571478	21.788435350647827
25	21.834420943296962	26.118692563029004	25.07963288787916	26.967253605794873
26	21.703736156205483	29.34175819855031	27.67885346809961	21.275652177144597
27	22.05024996149515	25.445076809428464	31.05428681108152	21.45038641799486
28	25.44093842725052	25.6874428592422	27.442438063169238	21.429180650338044
29	25.51907677189857	25.723481653042352	27.402526991588395	21.354914583470684
30	25.65590948816792	25.44585218258597	27.33292233967979	21.565315989566322
31	24.34107389732876	26.20714474707396	27.62154467454675	21.830236681050526
32	23.38815405077189	26.797483588355398	28.074036850045168	21.740325510827535
33	23.409057009976667	26.258576303853047	27.991934467479453	22.340432218690832
34	22.787996874833254	26.658136644332707	28.103946066694846	22.44992041413919
35	22.492767810103498	26.65835686866147	28.47392293901112	22.374952382223913
36	23.252661032502495	26.4230150573338	28.10622630609888	22.218097604064827
37	23.09984370037067	26.71838176226903	28.118861676961465	22.062912860398836
38	23.014965573661208	26.55911828051413	28.5117097634208	21.914206382403854
39	22.990933593785307	26.33107598808348	28.38612683994559	22.291863578185627
40	22.669080325309874	26.882407595133355	28.190439171815214	22.25807290774156
41	22.83259688941394	26.841271525723787	28.230524587656326	22.095606997205948
42	22.907840201740168	26.651268398079512	28.16177789302851	22.279113507151806
43	22.64451154863262	26.874874087887036	28.438292477820543	22.042321885659806
44	22.824746809695025	26.645482921442724	28.434250443786436	22.095519825075815
45	23.41718695811338	26.22493703763501	28.03285948857396	22.325016515677657
46	22.797608749182245	26.659026717661444	28.062149324298996	22.481215208857314
47	22.95851932539599	26.54330342090508	28.16423706469966	22.33394018899927
48	22.69504385606927	26.3637058927947	28.22220194323195	22.719048307904078
49	22.50569222539758	26.900443050057405	28.069870939826135	22.523993784718883
50	23.067085331467666	26.809577574409786	27.964232082124223	22.159105011998324
51	23.062336744378786	26.335356598473748	28.08301099144213	22.519295665705343
52	23.336176521177755	26.60390640337564	27.872205840743767	22.18771123470284
53	23.15390418507432	26.774139809506874	27.760487873966717	22.311468131452088
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4010.0
1	3036.5
2	2063.0
3	1969.0
4	1875.0
5	2070.0
6	2265.0
7	2589.5
8	3631.5
9	4349.0
10	5755.0
11	7161.0
12	9939.0
13	12717.0
14	17069.5
15	21422.0
16	29930.0
17	38438.0
18	51738.0
19	65038.0
20	87569.5
21	110101.0
22	335231.0
23	560361.0
24	424907.0
25	308444.5
26	327436.0
27	372374.5
28	417313.0
29	464631.0
30	511949.0
31	556037.5
32	600126.0
33	659109.5
34	718093.0
35	792877.5
36	867662.0
37	940747.5
38	1013833.0
39	1087183.0
40	1160533.0
41	1240768.5
42	1372681.0
43	1424358.0
44	1478466.0
45	1532574.0
46	1552223.0
47	1571872.0
48	1581534.5
49	1591197.0
50	1557435.0
51	1523673.0
52	1453219.5
53	1382766.0
54	1300530.0
55	1218294.0
56	1142087.0
57	1065880.0
58	929769.5
59	685108.0
60	576557.0
61	494153.5
62	411750.0
63	344597.5
64	277445.0
65	224844.0
66	172243.0
67	134636.5
68	97030.0
69	73424.5
70	49819.0
71	36799.5
72	23780.0
73	17872.0
74	11964.0
75	8685.5
76	3996.5
77	2586.0
78	2045.5
79	1505.0
80	1139.5
81	774.0
82	591.5
83	409.0
84	274.5
85	140.0
86	104.5
87	69.0
88	50.5
89	32.0
90	30.0
91	28.0
92	21.0
93	11.5
94	9.0
95	8.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.1795957E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.03612007144008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.90302114334709	36.895181216430636
2	15.851585826368883	16.497100467673377
3	5.63396819289261	8.795095360921025
4	2.6181469598479055	5.449528382692857
5	1.4304390679457217	3.721724954725121
6	0.8680559239357989	2.710215737198886
7	0.5825041979707661	2.1217880871407284
8	0.4013900524930157	1.670942477360656
9	0.2948525210003057	1.3808683067524843
>10	1.3047851454935158	11.592741977342321
>50	0.07306197594786462	2.6067081962066943
>100	0.0356928430694109	3.28731932782673
>500	0.0017992022687412916	0.6303299092306244
>1k	6.088680371110041E-4	0.5825726539370042
>5k	3.5231752568861065E-5	0.12801182784129903
>10k+	5.2847628853264645E-5	1.9298711167194529
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	335523	1.5393818220507591	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	27928	0.1281338552833445	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4775236985464784E-4	0.0	0.0	0.0	0.0
2	0.004055798054657568	0.0	0.0	1.3764020547480434E-5	0.0
3	0.004078738088903368	0.0	0.0	1.3764020547480434E-5	4.588006849160145E-6
4	0.004606358876556785	0.0	0.0	1.3764020547480434E-5	4.588006849160145E-6
5	0.004620122897104266	0.0	0.0	1.835202739664058E-5	1.835202739664058E-5
6	0.004620122897104266	0.0	0.0	1.835202739664058E-5	1.835202739664058E-5
7	0.004624710903953426	0.0	0.0	1.835202739664058E-5	1.835202739664058E-5
8	0.004624710903953426	0.0	0.0	2.2940034245800725E-5	2.2940034245800725E-5
9	0.004647650938199226	0.0	0.0	2.2940034245800725E-5	2.2940034245800725E-5
10	0.004661414958746707	0.0	0.0	3.211604794412101E-5	3.211604794412101E-5
11	0.004666002965595867	4.588006849160145E-6	0.0	3.670405479328116E-5	3.211604794412101E-5
12	0.004675178979294188	9.17601369832029E-6	0.0	3.670405479328116E-5	3.211604794412101E-5
13	0.0046889429998416676	9.17601369832029E-6	0.0	3.670405479328116E-5	3.211604794412101E-5
14	0.004702707020389148	9.17601369832029E-6	0.0	3.670405479328116E-5	3.211604794412101E-5
15	0.004721059047785789	9.17601369832029E-6	0.0	4.1292061642441304E-5	3.211604794412101E-5
16	0.0047394110751824295	9.17601369832029E-6	0.0	5.046807534076159E-5	3.670405479328116E-5
17	0.00476693911627739	9.17601369832029E-6	0.0	5.046807534076159E-5	4.588006849160145E-5
18	0.004812819184768992	9.17601369832029E-6	0.0	5.5056082189921735E-5	5.5056082189921735E-5
19	0.0048449352327131125	9.17601369832029E-6	0.0	5.5056082189921735E-5	9.17601369832029E-5
20	0.004904579321752194	9.17601369832029E-6	0.0	5.964408903908188E-5	9.634814383236303E-5
21	0.004945871383394636	9.17601369832029E-6	0.0	5.964408903908188E-5	9.634814383236303E-5
22	0.004977987431338757	9.17601369832029E-6	0.0	5.964408903908188E-5	9.634814383236303E-5
23	0.005037631520377839	9.17601369832029E-6	0.0	6.423209588824203E-5	1.0093615068152318E-4
24	0.005106451623115241	9.17601369832029E-6	0.0	6.882010273740217E-5	1.0093615068152318E-4
25	0.005189035746400124	9.17601369832029E-6	0.0	7.799611643572246E-5	1.1011216437984347E-4
26	0.005299147910779967	9.17601369832029E-6	0.0	8.717213013404275E-5	1.1011216437984347E-4
27	0.00539090804776317	9.17601369832029E-6	0.0	9.17601369832029E-5	1.1011216437984347E-4
28	0.0054689041641988925	9.17601369832029E-6	0.0	9.17601369832029E-5	1.2846419177648405E-4
29	0.005556076294332935	9.17601369832029E-6	0.0	1.0093615068152318E-4	1.2846419177648405E-4
30	0.005647836431316138	9.17601369832029E-6	0.0	1.0093615068152318E-4	1.2846419177648405E-4
31	0.005767124609394302	9.17601369832029E-6	0.0	1.0093615068152318E-4	1.3305219862564418E-4
32	0.005909352821718266	9.17601369832029E-6	0.0	1.1011216437984347E-4	1.3764020547480434E-4
33	0.006079109075137192	9.17601369832029E-6	0.0	1.1011216437984347E-4	1.3764020547480434E-4
34	0.006225925294310317	9.17601369832029E-6	0.0	1.1928817807816376E-4	1.3764020547480434E-4
35	0.006441561616220843	9.17601369832029E-6	0.0	1.2387618492732392E-4	1.4681621917312463E-4
36	0.0066067298627906085	9.17601369832029E-6	0.0	1.2846419177648405E-4	1.5599223287144493E-4
37	0.006771898109360373	9.17601369832029E-6	0.0	1.3764020547480434E-4	1.6516824656976522E-4
38	0.006996710444969221	9.17601369832029E-6	0.0	1.3764020547480434E-4	1.6516824656976522E-4
39	0.007207758760030587	9.17601369832029E-6	0.0	1.5140422602228477E-4	1.6516824656976522E-4
40	0.007418807075091954	9.17601369832029E-6	0.0	1.743442602680855E-4	1.743442602680855E-4
41	0.007657383431248281	9.17601369832029E-6	0.0	1.7893226711724564E-4	1.7893226711724564E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	141480	0.0	42.967064	1
TCAACGC	147270	0.0	41.66395	12
CAACGCA	147565	0.0	41.587025	13
GTGGTAT	147035	0.0	41.521164	6
ATCAACG	147855	0.0	41.50228	11
GGTATCA	147485	0.0	41.482117	8
TGGTATC	147860	0.0	41.302208	7
AGTGGTA	147975	0.0	41.276466	5
GTATCAA	148340	0.0	41.250942	9
AACGCAG	149185	0.0	41.15591	14
CGCAGAG	149280	0.0	41.071476	16
TATCAAC	149020	0.0	41.05009	10
ACGCAGA	149785	0.0	40.953396	15
AGAGTAC	147585	0.0	40.438118	19
CAGAGTA	150425	0.0	40.18082	18
GCAGTGG	152905	0.0	40.110065	3
AGCAGTG	153125	0.0	40.080063	2
CAGTGGT	152610	0.0	40.055172	4
GAGTACT	57265	0.0	39.55994	20
GCAGAGT	153965	0.0	39.450817	17
>>END_MODULE