FastQCFastQC Report
Thu 26 May 2016
SRR805232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23901064
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3419511.430693629371479No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG740670.3098899697519742No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG487710.2040536772756225No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC297160.12432919304345613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2339450.044.3993261
TCAACGC2403650.043.25353212
CAACGCA2405150.043.22069513
GTGGTAT2395550.043.2006456
GTATCAA2406250.043.1335539
ATCAACG2410950.043.1176911
AGTGGTA2401500.043.115145
GGTATCA2408450.043.091238
AACGCAG2418600.042.98034314
TATCAAC2415200.042.9318710
TGGTATC2415150.042.9142727
ACGCAGA2423900.042.84467315
CGCAGAG2422300.042.82058716
AGAGTAC2368800.042.6567519
AGCAGTG2451800.042.4491422
CAGAGTA2415550.042.38668418
GCAGTGG2453150.042.3797953
CAGTGGT2458550.042.17874
GTACATG1820350.041.78839522
GCAGAGT2461050.041.78255517