##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805232_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23901064
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.38757893790837	37.0	37.0	37.0	35.0	37.0
2	36.294717339780355	37.0	37.0	37.0	35.0	37.0
3	37.98515442659791	39.0	39.0	39.0	35.0	39.0
4	37.95454248396641	39.0	38.0	39.0	35.0	39.0
5	37.91046348396875	39.0	38.0	39.0	35.0	39.0
6	37.782325799387	39.0	38.0	39.0	35.0	39.0
7	37.71170128660381	39.0	38.0	39.0	35.0	39.0
8	38.75663614808111	40.0	39.0	40.0	36.0	40.0
9	38.81479941646113	40.0	39.0	40.0	36.0	40.0
10	38.46553789404522	40.0	38.0	40.0	36.0	40.0
11	38.663919187865446	40.0	39.0	40.0	36.0	40.0
12	38.80484345801509	40.0	39.0	40.0	36.0	40.0
13	38.85093420945611	40.0	39.0	40.0	36.0	40.0
14	38.86986399433933	40.0	39.0	40.0	36.0	40.0
15	38.82765867661791	40.0	39.0	40.0	36.0	40.0
16	38.80975026885832	40.0	39.0	40.0	36.0	40.0
17	38.7971235506503	40.0	39.0	40.0	36.0	40.0
18	38.79028285100613	40.0	39.0	40.0	36.0	40.0
19	38.71992422596751	40.0	39.0	40.0	36.0	40.0
20	38.68540032360065	40.0	39.0	40.0	36.0	40.0
21	38.57116536736607	40.0	39.0	40.0	36.0	40.0
22	38.52898398163362	40.0	39.0	40.0	36.0	40.0
23	38.092629516409815	40.0	38.0	40.0	34.0	40.0
24	38.23806346027106	40.0	38.0	40.0	35.0	40.0
25	38.09349140272584	40.0	38.0	40.0	34.0	40.0
26	38.14356729056079	40.0	38.0	40.0	34.0	40.0
27	38.19901339957083	40.0	38.0	40.0	34.0	40.0
28	38.18571068635271	40.0	38.0	40.0	34.0	40.0
29	38.12146208219015	40.0	38.0	40.0	34.0	40.0
30	38.05460576985192	40.0	38.0	40.0	34.0	40.0
31	37.80302249305721	40.0	38.0	40.0	33.0	40.0
32	37.857458270477	40.0	38.0	40.0	33.0	40.0
33	37.761506768066894	40.0	38.0	40.0	33.0	40.0
34	37.67277937919417	40.0	38.0	40.0	33.0	40.0
35	37.73590209205749	40.0	38.0	40.0	33.0	40.0
36	37.70647105919636	40.0	38.0	40.0	33.0	40.0
37	37.86859572444139	40.0	38.0	40.0	33.0	40.0
38	37.837801781544115	40.0	38.0	40.0	33.0	40.0
39	37.92209422141207	40.0	38.0	40.0	34.0	40.0
40	37.887174186052974	40.0	38.0	40.0	34.0	40.0
41	37.85181513258154	40.0	38.0	40.0	34.0	40.0
42	37.83468263170209	40.0	38.0	40.0	34.0	40.0
43	37.894566534778534	40.0	38.0	40.0	34.0	40.0
44	37.86247089250922	40.0	38.0	40.0	34.0	40.0
45	37.65463311591484	40.0	37.0	40.0	33.0	40.0
46	37.724829446923366	40.0	37.0	40.0	33.0	40.0
47	37.65927558706173	40.0	37.0	40.0	33.0	40.0
48	37.69540611246428	40.0	37.0	40.0	34.0	40.0
49	37.49712347533984	40.0	37.0	40.0	33.0	40.0
50	37.43510339121304	40.0	37.0	40.0	33.0	40.0
51	37.29297771011366	40.0	36.0	40.0	33.0	40.0
52	36.811307312511275	40.0	36.0	40.0	31.0	40.0
53	36.437721977565516	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	4.0
12	23.0
13	71.0
14	205.0
15	656.0
16	1375.0
17	2369.0
18	3735.0
19	5375.0
20	8111.0
21	12333.0
22	19283.0
23	29338.0
24	43594.0
25	61975.0
26	85471.0
27	113338.0
28	147455.0
29	188233.0
30	235073.0
31	291428.0
32	361377.0
33	453508.0
34	588287.0
35	811652.0
36	1223703.0
37	2176809.0
38	3871426.0
39	1.3164854E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.564344415796718	36.838657057275775	31.13215378194042	19.464844744987083
2	28.4986936146441	43.58740263613369	12.508221391315466	15.405682357906745
3	35.74724957851249	26.04203729172894	18.288499624953936	19.92221350480464
4	22.297409019113125	24.547082924843846	19.582019444824716	33.57348861121831
5	20.712672038366158	33.97636188916108	23.30975307208081	22.00121300039195
6	31.290653001891467	23.732152677387084	21.981946075706084	22.995248245015368
7	22.56397037387122	23.12032635869265	32.28426567118518	22.031437596250942
8	30.384145241400134	25.10168584963414	23.375925858363463	21.138243050602266
9	30.78454164216288	24.371651404305684	23.534830917987584	21.308976035543857
10	21.36584379674478	24.94029554500168	32.4854742868351	21.208386371418445
11	21.11777115863963	33.8825041429118	23.846812844817286	21.152911853631288
12	20.940712095494995	24.51142342449692	32.931019305249336	21.616845174758748
13	21.070936423583486	24.625435085233025	23.950653410241486	30.352975080942002
14	21.455040662624896	33.91757789527696	23.527366815134254	21.100014626963887
15	21.14719244298078	33.55728431169424	23.550382526903405	21.745140718421574
16	21.636032605075656	24.239715855327614	23.746880055214277	30.377371484382454
17	30.718439982420865	24.024348874175644	23.74547007614389	21.5117410672596
18	21.36845874309194	24.297884813830883	23.711664049767826	30.62199239330935
19	21.628790249672566	33.33267924808703	23.68194570752164	21.356584794718763
20	30.85505733133889	24.4921146606695	23.613915263353967	21.038912744637646
21	21.772490965255773	33.21759650532713	23.649097797487176	21.360814731929924
22	30.422599596402904	24.04039418496181	24.1258548155011	21.411151403134188
23	21.67955786403484	24.40255797817202	32.69639376723982	21.221490390553324
24	21.628275628231446	32.962197833535775	23.697422005982663	21.712104532250112
25	21.847993880105086	24.300156679217295	23.645495447399327	30.20635399327829
26	21.728944786725812	30.960291140176853	25.91480027834744	21.39596379474989
27	21.952407641768584	24.03581698287574	32.697648941486456	21.31412643386922
28	28.682702159200947	23.990919400073572	25.90960804088052	21.41677039984496
29	28.852665303937936	24.142900081770417	25.678522094246514	21.325912520045133
30	28.711445649448912	23.979614464025534	25.744372719139196	21.564567167386357
31	26.309556762828635	24.99270325371289	26.341061636419198	22.356678347039278
32	24.788854588230883	26.097992959643975	26.818801874259655	22.294350577865487
33	24.458400680404853	25.572384559950972	27.041034658540724	22.92818010110345
34	23.669866747354845	25.45286268427213	27.4557233100585	23.421547258314526
35	23.302506532763562	25.717110334502262	27.744777387316315	23.23560574541786
36	23.90507803334613	25.67187803856766	27.50804315657244	22.91500077151377
37	24.159079277809557	25.594588592373963	27.507946926546868	22.738385203269612
38	24.08523737687996	25.666024742664177	27.6001101875632	22.648627692892667
39	23.982999250577297	25.50340436727001	27.609356637846748	22.904239744305944
40	23.488795310535128	26.105030303253447	27.39590170546382	23.0102726807476
41	23.823370373804277	26.302766270154333	27.135687348479546	22.73817600756184
42	23.71261798219527	26.267939368724335	27.26646395323656	22.752978695843833
43	23.506413773043743	25.928075837962695	27.902456560092894	22.663053828900672
44	23.852164071022113	25.71587189591225	27.477324858843104	22.95463917422254
45	24.340297151624714	25.300191656739635	27.251975058516226	23.10753613311943
46	23.667770606362964	25.491547154553455	27.199709602886298	23.640972636197283
47	23.67152776127456	25.594450523206834	27.395039819147797	23.338981896370807
48	23.376670595083134	25.463715757591377	27.523435776750354	23.636177870575136
49	23.101025962693544	26.15728739105506	27.295839214522	23.4458474317294
50	23.954833140482783	25.970437968786662	27.0474988059109	23.027230084819656
51	23.71441288136796	25.628164503471478	27.46265187189993	23.194770743260634
52	24.23197979805418	25.512449989674103	27.2493475604266	23.006222651845125
53	24.326741269761047	25.68176462771699	26.8223289138927	23.169165188629258
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2700.0
1	1725.5
2	751.0
3	648.5
4	546.0
5	578.0
6	610.0
7	750.5
8	1126.0
9	1361.0
10	2037.0
11	2713.0
12	4072.0
13	5431.0
14	7233.5
15	9036.0
16	13651.5
17	18267.0
18	26526.5
19	34786.0
20	50397.5
21	66009.0
22	281258.5
23	496508.0
24	359929.5
25	235523.5
26	247696.0
27	281316.0
28	314936.0
29	358224.0
30	401512.0
31	442600.5
32	483689.0
33	545409.5
34	607130.0
35	689843.0
36	772556.0
37	857326.0
38	942096.0
39	1032063.5
40	1122031.0
41	1218239.0
42	1397415.5
43	1480384.0
44	1567507.0
45	1654630.0
46	1705441.5
47	1756253.0
48	1814320.5
49	1872388.0
50	1857088.5
51	1841789.0
52	1783495.0
53	1725201.0
54	1656371.5
55	1587542.0
56	1540354.0
57	1493166.0
58	1291774.0
59	939373.0
60	788364.0
61	683785.5
62	579207.0
63	492694.0
64	406181.0
65	330684.5
66	255188.0
67	202886.5
68	150585.0
69	114856.5
70	79128.0
71	58095.0
72	37062.0
73	27379.0
74	17696.0
75	13058.5
76	6207.0
77	3993.0
78	2984.5
79	1976.0
80	1626.5
81	1277.0
82	977.5
83	678.0
84	477.5
85	277.0
86	192.0
87	107.0
88	84.0
89	61.0
90	52.0
91	43.0
92	29.0
93	11.5
94	8.0
95	6.0
96	4.0
97	4.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.3901064E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.92448276170846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19819011730876	36.790325561059475
2	12.775304067842757	12.500502872855138
3	4.458667484948434	6.544140015226489
4	2.1708358600422137	4.248280864525354
5	1.2178328812903947	2.9790921903666825
6	0.8015620500391458	2.352960521974793
7	0.5723669831554992	1.960193102053361
8	0.41206406399996287	1.6128016956708573
9	0.3527184750127325	1.5530912055446877
>10	1.8608822907628613	15.955345721609662
>50	0.11195492458946242	3.7875625768764243
>100	0.06392374019758729	5.589139768528641
>500	0.002762300946326807	0.9146345162606061
>1k	7.979267862777995E-4	0.7088521268419882
>5k	6.841653877150707E-5	0.20765463548934748
>10k+	6.841653877114027E-5	2.2954226251165903
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	341951	1.430693629371479	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	74067	0.3098899697519742	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	48771	0.2040536772756225	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	29716	0.12432919304345613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0583967307898926E-4	4.183914155453498E-6	0.0	0.0	8.367828310906995E-6
2	0.016526460914041315	4.183914155453498E-6	0.0	0.0	8.367828310906995E-6
3	0.01658085179806221	4.183914155453498E-6	0.0	0.0	1.2551742466360493E-5
4	0.017162415865670248	4.183914155453498E-6	0.0	0.0	1.673565662181399E-5
5	0.017162415865670248	4.183914155453498E-6	0.0	0.0	2.5103484932720986E-5
6	0.017162415865670248	4.183914155453498E-6	0.0	0.0	2.5103484932720986E-5
7	0.017170783693981156	4.183914155453498E-6	0.0	0.0	2.5103484932720986E-5
8	0.01717496760813661	4.183914155453498E-6	4.183914155453498E-6	4.183914155453498E-6	3.347131324362798E-5
9	0.01717496760813661	1.673565662181399E-5	4.183914155453498E-6	4.183914155453498E-6	4.602305570998848E-5
10	0.017195887178913877	1.673565662181399E-5	4.183914155453498E-6	4.183914155453498E-6	5.020696986544197E-5
11	0.01720425500722478	1.673565662181399E-5	4.183914155453498E-6	4.183914155453498E-6	5.4390884020895474E-5
12	0.017580807281215596	2.091957077726749E-5	4.183914155453498E-6	8.367828310906995E-6	5.4390884020895474E-5
13	0.017597542937837413	2.5103484932720986E-5	4.183914155453498E-6	1.2551742466360493E-5	6.275871233180247E-5
14	0.017635198165236492	2.9287399088174484E-5	4.183914155453498E-6	1.2551742466360493E-5	6.694262648725596E-5
15	0.017660301650169213	2.9287399088174484E-5	4.183914155453498E-6	1.2551742466360493E-5	6.694262648725596E-5
16	0.01766866947848012	3.347131324362798E-5	4.183914155453498E-6	2.091957077726749E-5	7.949436895361646E-5
17	0.01768958904925739	3.347131324362798E-5	4.183914155453498E-6	2.5103484932720986E-5	8.367828310906995E-5
18	0.017723060362501018	3.347131324362798E-5	1.2551742466360493E-5	2.5103484932720986E-5	8.367828310906995E-5
19	0.017769083418211006	3.765522739908148E-5	1.2551742466360493E-5	2.5103484932720986E-5	9.623002557543045E-5
20	0.017806738645610085	3.765522739908148E-5	1.2551742466360493E-5	2.5103484932720986E-5	1.2551742466360494E-4
21	0.01786949735794189	3.765522739908148E-5	1.2551742466360493E-5	2.5103484932720986E-5	1.2551742466360494E-4
22	0.017944807812740052	3.765522739908148E-5	1.2551742466360493E-5	2.5103484932720986E-5	1.2551742466360494E-4
23	0.01799919869676095	4.183914155453498E-5	1.2551742466360493E-5	2.5103484932720986E-5	1.3388525297451193E-4
24	0.01809542872233638	4.183914155453498E-5	1.2551742466360493E-5	2.5103484932720986E-5	1.3806916712996543E-4
25	0.01819165874791181	4.183914155453498E-5	1.2551742466360493E-5	2.5103484932720986E-5	1.3806916712996543E-4
26	0.01827533703102088	4.183914155453498E-5	1.2551742466360493E-5	2.5103484932720986E-5	1.4225308128541892E-4
27	0.018388302713218122	4.183914155453498E-5	1.2551742466360493E-5	3.347131324362798E-5	1.4643699544087242E-4
28	0.018497084481259914	4.602305570998848E-5	1.2551742466360493E-5	3.347131324362798E-5	1.6317265206268643E-4
29	0.018643521476700786	4.602305570998848E-5	1.2551742466360493E-5	3.347131324362798E-5	1.6317265206268643E-4
30	0.018789958472141658	5.020696986544197E-5	1.2551742466360493E-5	3.765522739908148E-5	1.757243945290469E-4
31	0.018915475896805263	5.4390884020895474E-5	1.673565662181399E-5	3.765522739908148E-5	1.757243945290469E-4
32	0.01905772897809068	5.4390884020895474E-5	1.673565662181399E-5	3.765522739908148E-5	1.840922228399539E-4
33	0.019241821200930636	5.4390884020895474E-5	1.673565662181399E-5	3.765522739908148E-5	1.882761369954074E-4
34	0.019404993852993324	5.4390884020895474E-5	1.673565662181399E-5	3.765522739908148E-5	1.882761369954074E-4
35	0.01961837347492145	5.857479817634897E-5	1.673565662181399E-5	4.183914155453498E-5	1.966439653063144E-4
36	0.019856856581782302	5.857479817634897E-5	1.673565662181399E-5	4.183914155453498E-5	2.1337962192812839E-4
37	0.020040948804622254	5.857479817634897E-5	2.091957077726749E-5	4.183914155453498E-5	2.2174745023903538E-4
38	0.02032127105303764	6.275871233180247E-5	2.091957077726749E-5	4.602305570998848E-5	2.2174745023903538E-4
39	0.020568121988209394	6.275871233180247E-5	2.091957077726749E-5	4.602305570998848E-5	2.2174745023903538E-4
40	0.020894467292334767	6.275871233180247E-5	2.091957077726749E-5	4.602305570998848E-5	2.2593136439448888E-4
41	0.021300306965413757	6.275871233180247E-5	2.091957077726749E-5	4.602305570998848E-5	2.2593136439448888E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	233945	0.0	44.399326	1
TCAACGC	240365	0.0	43.253532	12
CAACGCA	240515	0.0	43.220695	13
GTGGTAT	239555	0.0	43.200645	6
GTATCAA	240625	0.0	43.133553	9
ATCAACG	241095	0.0	43.11769	11
AGTGGTA	240150	0.0	43.11514	5
GGTATCA	240845	0.0	43.09123	8
AACGCAG	241860	0.0	42.980343	14
TATCAAC	241520	0.0	42.93187	10
TGGTATC	241515	0.0	42.914272	7
ACGCAGA	242390	0.0	42.844673	15
CGCAGAG	242230	0.0	42.820587	16
AGAGTAC	236880	0.0	42.65675	19
AGCAGTG	245180	0.0	42.449142	2
CAGAGTA	241555	0.0	42.386684	18
GCAGTGG	245315	0.0	42.379795	3
CAGTGGT	245855	0.0	42.1787	4
GTACATG	182035	0.0	41.788395	22
GCAGAGT	246105	0.0	41.782555	17
>>END_MODULE