FastQCFastQC Report
Thu 26 May 2016
SRR805231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18601327
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG175650.010.43552521
ACATGGG179200.010.30747723
AGTACAT183850.010.08512420
TACATGG187000.09.86497422
CATGGGG126300.08.87529724
AGAGTAC320450.07.96411318
GAGTACT65950.07.482941619
GAGTACA307500.06.68699219
ATGGGGG83750.06.622089425
GTACTTT82000.06.30487821
GGTATCA547000.05.64945137
CAACGCA551650.05.62313112
AGTGGTA553050.05.6046474
AGTACTT96400.05.58246920
GTGGTAT552800.05.5816755
ACGCAGA559200.05.57242514
TCAACGC557150.05.550749311
TGGGGGG66050.05.550340727
GTATCAA564700.05.54728178
AACGCAG562200.05.546869313
Produced by FastQC (version 0.11.5)