FastQCFastQC Report
Thu 26 May 2016
SRR805230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19943313
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2221741.1140275439692493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG567290.2844512343560972No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG344750.17286495979880573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1487900.044.07811
TCAACGC1556400.042.17437412
CAACGCA1559850.042.08712413
GTGGTAT1552000.042.063796
AGTGGTA1553800.042.0332075
ATCAACG1562000.042.0216711
GTATCAA1560700.041.9708449
GGTATCA1561300.041.9456868
AACGCAG1566350.041.9154714
ACGCAGA1571450.041.75700815
TGGTATC1566950.041.750957
TATCAAC1569100.041.7326810
AGCAGTG1580050.041.552042
CGCAGAG1579650.041.51793316
GCAGTGG1582400.041.439843
AGAGTAC1545000.041.34478819
CAGAGTA1580250.040.95937318
CAGTGGT1606400.040.7285544
GCAGAGT1616700.040.2277817
GTACATG1196050.040.13105422
Produced by FastQC (version 0.11.5)