##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805230_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19943313
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.39417613312292	37.0	37.0	37.0	35.0	37.0
2	36.298917938057734	37.0	37.0	37.0	35.0	37.0
3	37.98453857691548	39.0	39.0	39.0	35.0	39.0
4	37.96348906523204	39.0	38.0	39.0	35.0	39.0
5	37.92531511690159	39.0	38.0	39.0	35.0	39.0
6	37.79101711937229	39.0	38.0	39.0	35.0	39.0
7	37.75778076591387	39.0	38.0	39.0	35.0	39.0
8	38.78166390910076	40.0	39.0	40.0	36.0	40.0
9	38.82283334769905	40.0	39.0	40.0	36.0	40.0
10	38.56132067926728	40.0	38.0	40.0	36.0	40.0
11	38.70853639011733	40.0	39.0	40.0	36.0	40.0
12	38.819594517721306	40.0	39.0	40.0	36.0	40.0
13	38.85883744591483	40.0	39.0	40.0	36.0	40.0
14	38.86803235751252	40.0	39.0	40.0	36.0	40.0
15	38.82997253264791	40.0	39.0	40.0	36.0	40.0
16	38.81415780818362	40.0	39.0	40.0	36.0	40.0
17	38.807539248870036	40.0	39.0	40.0	36.0	40.0
18	38.796018695589844	40.0	39.0	40.0	36.0	40.0
19	38.730599474620895	40.0	39.0	40.0	36.0	40.0
20	38.687466019311834	40.0	39.0	40.0	36.0	40.0
21	38.59315922083758	40.0	39.0	40.0	36.0	40.0
22	38.55322924531145	40.0	39.0	40.0	36.0	40.0
23	38.21849880207967	40.0	38.0	40.0	34.0	40.0
24	38.317106139787306	40.0	38.0	40.0	35.0	40.0
25	38.136183842674484	40.0	38.0	40.0	34.0	40.0
26	38.17644159724114	40.0	38.0	40.0	34.0	40.0
27	38.222845572347985	40.0	38.0	40.0	35.0	40.0
28	38.20782289281625	40.0	38.0	40.0	35.0	40.0
29	38.1418185634453	40.0	38.0	40.0	34.0	40.0
30	38.081859869521175	40.0	38.0	40.0	34.0	40.0
31	37.85738377570467	40.0	38.0	40.0	34.0	40.0
32	37.91093305309905	40.0	38.0	40.0	34.0	40.0
33	37.823607542036775	40.0	38.0	40.0	33.0	40.0
34	37.737992428840684	40.0	38.0	40.0	33.0	40.0
35	37.81024251086066	40.0	38.0	40.0	33.0	40.0
36	37.75367858890847	40.0	38.0	40.0	33.0	40.0
37	37.93274597856434	40.0	38.0	40.0	34.0	40.0
38	37.92447398283324	40.0	38.0	40.0	34.0	40.0
39	37.99878525699316	40.0	38.0	40.0	34.0	40.0
40	37.95839813575608	40.0	38.0	40.0	34.0	40.0
41	37.93143095131686	40.0	38.0	40.0	34.0	40.0
42	37.92150015396138	40.0	38.0	40.0	34.0	40.0
43	37.96218025560748	40.0	38.0	40.0	34.0	40.0
44	37.93470969442239	40.0	38.0	40.0	34.0	40.0
45	37.72749507566772	40.0	38.0	40.0	33.0	40.0
46	37.78677258888731	40.0	38.0	40.0	34.0	40.0
47	37.728103048876584	40.0	37.0	40.0	34.0	40.0
48	37.76124528557517	40.0	37.0	40.0	34.0	40.0
49	37.57759049361558	40.0	37.0	40.0	33.0	40.0
50	37.51461966223967	40.0	37.0	40.0	33.0	40.0
51	37.388760483275775	40.0	37.0	40.0	33.0	40.0
52	36.932785741265754	40.0	36.0	40.0	32.0	40.0
53	36.57321298622752	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	13.0
13	46.0
14	131.0
15	425.0
16	929.0
17	1775.0
18	2867.0
19	4184.0
20	6345.0
21	9811.0
22	15276.0
23	23900.0
24	34924.0
25	49812.0
26	68848.0
27	92378.0
28	119717.0
29	152728.0
30	191778.0
31	239395.0
32	295764.0
33	370831.0
34	478796.0
35	660324.0
36	988968.0
37	1735341.0
38	3167718.0
39	1.1230287E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.955148124085502	33.8498122152523	28.768234244731556	22.42680541593064
2	26.24755977103704	36.67332002461176	18.68293898812098	18.39618121623022
3	31.466446923838582	25.40721794819146	21.14428029084235	21.982054837127613
4	22.06831934092395	25.071371040508666	21.98720944709638	30.87310017147101
5	22.568928241762038	31.980173003352053	23.75822913675376	21.69266961813215
6	30.120351618610208	24.400649982277265	23.061213550627222	22.417784848485304
7	22.943319397333834	24.131527194102606	31.198011082712284	21.727142325851275
8	28.882543236422155	25.32599272748715	24.222329559787784	21.56913447630291
9	28.740987016550363	24.83907763970811	24.747823994940056	21.672111348801476
10	21.68147288266498	25.26321479284811	31.315047805748225	21.740264518738687
11	21.62279657346801	32.10322678082623	24.558181481682606	21.71579516402315
12	21.4667643234602	25.024473115374562	31.48958249815364	22.019180063011596
13	21.53330793133518	25.162368960463088	24.557434364089858	28.746888744111875
14	21.887832778836696	32.16728333953341	24.336778949415276	21.608104932214623
15	21.63846097185558	31.844242729380017	24.352468418863005	22.1648278799014
16	21.993321771563227	24.814442815995516	24.466657069464837	28.725578342976416
17	29.088361597694423	24.598831698625	24.435874821801175	21.876931881879404
18	21.857110701717414	24.885293632005876	24.42496389641982	28.832631769856892
19	22.144670747533272	31.74925851086026	24.415597348344278	21.690473393262195
20	29.104417104620484	24.875736543873128	24.44020208678468	21.579644264721715
21	22.10400047374275	31.659338646492685	24.446454809188424	21.790206070576136
22	28.760076121755695	24.544577924440137	24.80716218012524	21.888183773678925
23	22.023492285359005	24.905731560247787	31.360611950481847	21.710164203911354
24	21.993712880101715	31.447618557658902	24.482105856735036	22.076562705504347
25	22.38259510844562	24.729492035751534	24.37206395948356	28.515848896319284
26	22.24150019608076	29.808036407992994	26.234206924396165	21.71625647153008
27	22.375966320139486	24.5424167990544	31.327879174337784	21.75373770646833
28	27.51128160100581	24.589264582068186	26.096767372602535	21.80268644432347
29	27.573833896103423	24.66128370948197	25.987277038674566	21.777605355740043
30	27.440310443906686	24.562112623915596	26.00367852623082	21.993898405946897
31	25.792770739746203	25.274291187226517	26.3994151824223	22.53352289060499
32	24.654705063296152	26.030855555443573	26.743741122650988	22.57069825860929
33	24.397806924055196	25.69025517475457	26.910323274773855	23.001614626416384
34	23.731152391781645	25.58026843383544	27.266738480211387	23.421840694171525
35	23.386289930865548	25.750882012431937	27.57398432246438	23.28884373423814
36	23.955337811726668	25.78602662456333	27.299586583232184	22.959048980477817
37	24.163768577467547	25.729401128087392	27.254839755059752	22.851990539385305
38	24.02915202704786	25.833385857204366	27.363071521767722	22.774390593980048
39	23.98678694959057	25.743165140114883	27.357641130137207	22.912406780157337
40	23.5699855886532	26.259709206790266	27.15229911900796	23.018006085548574
41	23.864826270339336	26.31847075759178	27.00228893765043	22.814414034418455
42	23.7493790525175	26.337800544974648	27.06336705441067	22.849453348097178
43	23.564379699601563	26.03239993274939	27.600118395574498	22.80310197207455
44	23.843636210292644	25.768080759701263	27.333392400751066	23.054890629255027
45	24.31402445521464	25.450560796994964	27.089606425973457	23.14580832181694
46	23.697868052314078	25.63025511358118	27.049838710348677	23.622038123756067
47	23.690717785956625	25.677278394016078	27.27111588731521	23.36088793271208
48	23.37523359333527	25.628139116103725	27.32788679594007	23.66874049462093
49	23.20463505737487	26.235580818492892	27.142225567035926	23.417558557096303
50	23.95084006353408	26.025685902838713	26.929999042786925	23.09347499084029
51	23.76375981262491	25.758182705150347	27.270158172817123	23.20789930940762
52	24.280494419357506	25.646581387956957	27.025168787151866	23.047755405533675
53	24.31660677441105	25.785449990179664	26.75917486728509	23.138768368124193
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1802.0
1	1192.0
2	582.0
3	524.0
4	466.0
5	478.5
6	491.0
7	615.0
8	1006.0
9	1273.0
10	1851.5
11	2430.0
12	3750.5
13	5071.0
14	6966.5
15	8862.0
16	13408.5
17	17955.0
18	25846.0
19	33737.0
20	48187.5
21	62638.0
22	207693.5
23	352749.0
24	268649.5
25	199140.0
26	213730.0
27	244126.0
28	274522.0
29	311579.0
30	348636.0
31	385331.0
32	422026.0
33	473366.0
34	524706.0
35	591052.5
36	657399.0
37	725811.5
38	794224.0
39	864760.5
40	935297.0
41	1014401.0
42	1162010.5
43	1230516.0
44	1299747.5
45	1368979.0
46	1416904.0
47	1464829.0
48	1506479.0
49	1548129.0
50	1543841.5
51	1539554.0
52	1496070.0
53	1452586.0
54	1396515.0
55	1340444.0
56	1288635.0
57	1236826.0
58	1074875.5
59	786403.0
60	659881.0
61	570688.0
62	481495.0
63	406940.0
64	332385.0
65	269066.5
66	205748.0
67	162801.0
68	119854.0
69	90529.5
70	61205.0
71	44398.0
72	27591.0
73	20008.5
74	12426.0
75	9152.5
76	4191.0
77	2503.0
78	1827.0
79	1151.0
80	845.0
81	539.0
82	409.5
83	280.0
84	197.5
85	115.0
86	81.5
87	48.0
88	33.0
89	18.0
90	14.0
91	10.0
92	7.5
93	2.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.9943313E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.73803417904617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0855468374397	40.886877164817534
2	12.846925816026806	13.807370771946381
3	4.237714423475318	6.831793275892608
4	2.0305389438156607	4.364686846586011
5	1.1399786995003804	3.0630107158568025
6	0.7639026564695215	2.4630376237693987
7	0.529393394343745	1.9913992223583805
8	0.3963688886074489	1.7040067906798104
9	0.30436244318047795	1.472025543700547
>10	1.552240718436167	14.402468364783019
>50	0.07875981176683451	2.8818513899165357
>100	0.03210190756583573	3.1491589350644755
>500	0.0016141747496078568	0.5498722178454639
>1k	4.3922433705092433E-4	0.4812152387917083
>5k	6.536849990698274E-5	0.22731164968736603
>10k+	4.669178564782217E-5	1.7239142483038872
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	222174	1.1140275439692493	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	56729	0.2844512343560972	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	34475	0.17286495979880573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2090957003984245E-4	0.0	0.0	0.0	2.0056848127490153E-5
2	4.5629329490040095E-4	0.0	0.0	0.0	2.0056848127490153E-5
3	4.5629329490040095E-4	0.0	0.0	0.0	3.0085272191235226E-5
4	4.613075069322735E-4	0.0	0.0	0.0	4.0113696254980306E-5
5	4.713359309960186E-4	0.0	0.0	0.0	6.518475641434299E-5
6	4.964069911553812E-4	5.014212031872538E-6	0.0	0.0	7.521318047808807E-5
7	5.064354152191263E-4	5.014212031872538E-6	0.0	0.0	8.022739250996061E-5
8	5.064354152191263E-4	5.014212031872538E-6	0.0	0.0	8.524160454183314E-5
9	5.264922633466165E-4	5.014212031872538E-6	0.0	0.0	9.025581657370569E-5
10	5.31506475378489E-4	5.014212031872538E-6	0.0	0.0	9.527002860557822E-5
11	5.31506475378489E-4	5.014212031872538E-6	0.0	0.0	1.2535530079681344E-4
12	5.415348994422341E-4	5.014212031872538E-6	0.0	0.0	1.3036951282868598E-4
13	5.515633235059792E-4	5.014212031872538E-6	0.0	0.0	1.5042636095617614E-4
14	5.615917475697243E-4	5.014212031872538E-6	0.0	0.0	1.5544057298804868E-4
15	5.916770197609595E-4	5.014212031872538E-6	0.0	0.0	1.6045478501992123E-4
16	6.067196558565771E-4	5.014212031872538E-6	0.0	1.0028424063745077E-5	1.9555426924302899E-4
17	6.217622919521947E-4	5.014212031872538E-6	0.0	1.0028424063745077E-5	2.005684812749015E-4
18	6.468333521115574E-4	5.014212031872538E-6	0.0	1.0028424063745077E-5	2.105969053386466E-4
19	6.819328363346652E-4	5.014212031872538E-6	0.0	1.5042636095617613E-5	2.3065375346613675E-4
20	7.37089168685263E-4	5.014212031872538E-6	0.0	1.5042636095617613E-5	2.3065375346613675E-4
21	7.772028649402434E-4	5.014212031872538E-6	0.0	1.5042636095617613E-5	2.456963895617544E-4
22	8.323591972908413E-4	5.014212031872538E-6	0.0	2.0056848127490153E-5	2.6073902565737196E-4
23	8.975439537051843E-4	5.014212031872538E-6	0.0	2.0056848127490153E-5	2.757816617529896E-4
24	9.677429221513998E-4	5.014212031872538E-6	0.0	2.0056848127490153E-5	2.8581008581673463E-4
25	0.0010981124349800859	1.0028424063745077E-5	0.0	2.0056848127490153E-5	3.0085272191235227E-4
26	0.0011983966756175365	1.5042636095617613E-5	0.0	2.0056848127490153E-5	3.058669339442248E-4
27	0.0013036951282868598	1.5042636095617613E-5	0.0	2.0056848127490153E-5	3.2592378207171494E-4
28	0.0014089935809561832	1.5042636095617613E-5	0.0	2.0056848127490153E-5	3.459806301992051E-4
29	0.001509277821593634	1.5042636095617613E-5	0.0	2.0056848127490153E-5	3.5099484223107767E-4
30	0.0017098463028685355	1.5042636095617613E-5	0.0	2.0056848127490153E-5	3.710516903585678E-4
31	0.001900386360079692	1.5042636095617613E-5	0.0	2.0056848127490153E-5	3.810801144223129E-4
32	0.002095940629322721	1.5042636095617613E-5	0.0	2.0056848127490153E-5	3.8609432645418543E-4
33	0.0022714380504382597	2.0056848127490153E-5	0.0	2.5071060159362688E-5	4.0615117458167555E-4
34	0.0025221486520318865	3.0085272191235226E-5	0.0	2.5071060159362688E-5	4.111653866135481E-4
35	0.002757816617529896	3.0085272191235226E-5	0.0	2.5071060159362688E-5	4.412506588047833E-4
36	0.0030135414311553955	3.0085272191235226E-5	0.0	2.5071060159362688E-5	4.5629329490040095E-4
37	0.003254223608685277	3.0085272191235226E-5	0.0	2.5071060159362688E-5	4.613075069322735E-4
38	0.003524991058406394	3.509948422310777E-5	0.0	3.509948422310777E-5	4.613075069322735E-4
39	0.0038057869321912563	4.0113696254980306E-5	0.0	3.509948422310777E-5	4.613075069322735E-4
40	0.004262080227091658	4.0113696254980306E-5	0.0	3.509948422310777E-5	4.6632171896414604E-4
41	0.004678259825737078	4.0113696254980306E-5	0.0	3.509948422310777E-5	4.8637856709163617E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	148790	0.0	44.0781	1
TCAACGC	155640	0.0	42.174374	12
CAACGCA	155985	0.0	42.087124	13
GTGGTAT	155200	0.0	42.06379	6
AGTGGTA	155380	0.0	42.033207	5
ATCAACG	156200	0.0	42.02167	11
GTATCAA	156070	0.0	41.970844	9
GGTATCA	156130	0.0	41.945686	8
AACGCAG	156635	0.0	41.91547	14
ACGCAGA	157145	0.0	41.757008	15
TGGTATC	156695	0.0	41.75095	7
TATCAAC	156910	0.0	41.73268	10
AGCAGTG	158005	0.0	41.55204	2
CGCAGAG	157965	0.0	41.517933	16
GCAGTGG	158240	0.0	41.43984	3
AGAGTAC	154500	0.0	41.344788	19
CAGAGTA	158025	0.0	40.959373	18
CAGTGGT	160640	0.0	40.728554	4
GCAGAGT	161670	0.0	40.22778	17
GTACATG	119605	0.0	40.131054	22
>>END_MODULE