FastQCFastQC Report
Thu 26 May 2016
SRR805229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25352397
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT464880.18336727686932325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT445850.035.462151
CAACGCA547400.028.78041513
TCAACGC548650.028.73197712
ATCAACG558700.028.34553311
GGTATCA564000.028.0458328
AACGCAG561450.028.0225314
ACGCAGA562850.027.97370315
AGTGGTA567150.027.956365
GTATCAA567250.027.9390019
GTGGTAT568850.027.8191076
TATCAAC574600.027.589810
TGGTATC582350.027.2185967
CGCAGAG579450.027.17637316
AGAGTAC576050.026.794219
AGCAGTG603000.026.4267852
GCAGTGG613300.026.0442683
CAGTGGT617750.025.7653584
CAGAGTA608250.025.50324618
GCAGAGT636300.024.54887617
GTACATG487250.024.22585922
Produced by FastQC (version 0.11.5)